Affinity purification of macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using a peptide ligand derived from a novel serpin

Masahiro Nishibori, Naoki Nakaya, Shuji Mori, Masahiro Kawabata, Akihito Tahara, Kiyomi Saeki

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

We purified macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using an affinity column with the C-terminal region peptide of a novel serpin as a ligand. The affinity purified preparation showing a single band on SDS-PAGE contained four peptides on RP-HPLC, which were converged into two peptides time-dependently. Sequence analysis and Western blotting revealed that one was identical to bovine MIF/GIF and the other was an N-terminally modified form of MIF/GIF. These results indicated that there exist at least two forms of MIF/GIF in the bovine brain and that they have an affinity for the C-terminal portion of the serpin.

Original languageEnglish
Pages (from-to)259-262
Number of pages4
JournalJapanese Journal of Pharmacology
Volume71
Issue number3
Publication statusPublished - 1996

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Keywords

  • Brain
  • Macrophage migration inhibition factor/glycosylation inhibiting factor (MIF/GIF)
  • Serpin

ASJC Scopus subject areas

  • Pharmacology
  • Molecular Medicine

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