Affinity purification of macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using a peptide ligand derived from a novel serpin

Masahiro Nishibori; Naoki Nakaya; Shuji Mori; Masahiro Kawabata; Akihito Tahara; Kiyomi Saeki

doi:10.1254/jjp.71.259

Affinity purification of macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using a peptide ligand derived from a novel serpin

Masahiro Nishibori, Naoki Nakaya, Shuji Mori, Masahiro Kawabata, Akihito Tahara, Kiyomi Saeki

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

We purified macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using an affinity column with the C-terminal region peptide of a novel serpin as a ligand. The affinity purified preparation showing a single band on SDS-PAGE contained four peptides on RP-HPLC, which were converged into two peptides time-dependently. Sequence analysis and Western blotting revealed that one was identical to bovine MIF/GIF and the other was an N-terminally modified form of MIF/GIF. These results indicated that there exist at least two forms of MIF/GIF in the bovine brain and that they have an affinity for the C-terminal portion of the serpin.

Original language	English
Pages (from-to)	259-262
Number of pages	4
Journal	Japanese Journal of Pharmacology
Volume	71
Issue number	3
DOIs	https://doi.org/10.1254/jjp.71.259
Publication status	Published - 1996

Keywords

Brain
Macrophage migration inhibition factor/glycosylation inhibiting factor (MIF/GIF)
Serpin

ASJC Scopus subject areas

Pharmacology

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10.1254/jjp.71.259

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Affinity purification of macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using a peptide ligand derived from a novel serpin. / Nishibori, Masahiro; Nakaya, Naoki; Mori, Shuji et al.

In: Japanese Journal of Pharmacology, Vol. 71, No. 3, 1996, p. 259-262.

Research output: Contribution to journal › Article › peer-review

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AU - Mori, Shuji

AU - Kawabata, Masahiro

AU - Tahara, Akihito

AU - Saeki, Kiyomi

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Affinity purification of macrophage migration inhibitory factor/glycosylation inhibiting factor (MIF/GIF) from bovine brain by using a peptide ligand derived from a novel serpin

Abstract

Keywords

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