Abstract
A gene for FK506 binding protein 6 (Fkbp6) expresses during a specific stage of male and female meiosis. Disruption of the gene influences male reproduction, i.e. arrests spermatogenesis, but not female reproduction. Using the mouse model (targeted disruption), the role of the gene in homologous chromosome pairing has been demonstrated in a previous study. For further understanding the function of Fkbp6 in chromosome synapsis, we evaluated chromosome pairings during male meiosis in the as/as rat, a spontaneous null mutation, and compared them with those of the mouse model. Electron microscopy of the pachytene nuclei unveiled several types of abnormal chromosome pairing in the rat model, as shown in the mouse previously. The frequencies of aberrant pairings in the knockout mice and mutant rats were 42 of 67 nuclei (62.7%) and 20 out of 74 nuclei (27.0%), respectively. In order to clarify the mechanism of male specific infertility in Fkbp6 deficiency, the localization of γH2AX, a marker protein of XY chromosome inactivation during male meiosis, was examined. Immunostaining of γH2AX unveiled normal localization of the molecule to XY chromosomes (XY body) in both models, showing the independency of FKBP6 in sex chromosome inactivation. Besides the XY body, focal localization of γH2AX was observed in accordance with the unsynapsed chromosomes in both types of null animal. These results indicate the fundamental role of Fkbp6 in homologous chromosome synapsis during male meiosis. In conclusion, male specific infertility under Fkbp6 deficiency remains unsolved.
| Original language | English |
|---|---|
| Pages (from-to) | 203-207 |
| Number of pages | 5 |
| Journal | Journal of Reproduction and Development |
| Volume | 54 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jun 2008 |
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Keywords
- Chromosome pairing
- FK506 binding protein 6 (FKBP6)
- Meiosis
- Mouse
- Phosphorylated histone H2AX (γH2AX)
- Rat
ASJC Scopus subject areas
- Animal Science and Zoology
- Reproductive Medicine
Cite this
Affected homologous chromosome pairing and phosphorylation of testis specific histone, H2AX, in male meiosis under FKBP6 deficiency. / Noguchi, Junko; Ozawa, Manabu; Nakai, Michiko; Somfai, Tamás; Kikuchi, Kazuhiro; Kaneko, Hiroyuki; Kunieda, Tetsuo.
In: Journal of Reproduction and Development, Vol. 54, No. 3, 06.2008, p. 203-207.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Affected homologous chromosome pairing and phosphorylation of testis specific histone, H2AX, in male meiosis under FKBP6 deficiency
AU - Noguchi, Junko
AU - Ozawa, Manabu
AU - Nakai, Michiko
AU - Somfai, Tamás
AU - Kikuchi, Kazuhiro
AU - Kaneko, Hiroyuki
AU - Kunieda, Tetsuo
PY - 2008/6
Y1 - 2008/6
N2 - A gene for FK506 binding protein 6 (Fkbp6) expresses during a specific stage of male and female meiosis. Disruption of the gene influences male reproduction, i.e. arrests spermatogenesis, but not female reproduction. Using the mouse model (targeted disruption), the role of the gene in homologous chromosome pairing has been demonstrated in a previous study. For further understanding the function of Fkbp6 in chromosome synapsis, we evaluated chromosome pairings during male meiosis in the as/as rat, a spontaneous null mutation, and compared them with those of the mouse model. Electron microscopy of the pachytene nuclei unveiled several types of abnormal chromosome pairing in the rat model, as shown in the mouse previously. The frequencies of aberrant pairings in the knockout mice and mutant rats were 42 of 67 nuclei (62.7%) and 20 out of 74 nuclei (27.0%), respectively. In order to clarify the mechanism of male specific infertility in Fkbp6 deficiency, the localization of γH2AX, a marker protein of XY chromosome inactivation during male meiosis, was examined. Immunostaining of γH2AX unveiled normal localization of the molecule to XY chromosomes (XY body) in both models, showing the independency of FKBP6 in sex chromosome inactivation. Besides the XY body, focal localization of γH2AX was observed in accordance with the unsynapsed chromosomes in both types of null animal. These results indicate the fundamental role of Fkbp6 in homologous chromosome synapsis during male meiosis. In conclusion, male specific infertility under Fkbp6 deficiency remains unsolved.
AB - A gene for FK506 binding protein 6 (Fkbp6) expresses during a specific stage of male and female meiosis. Disruption of the gene influences male reproduction, i.e. arrests spermatogenesis, but not female reproduction. Using the mouse model (targeted disruption), the role of the gene in homologous chromosome pairing has been demonstrated in a previous study. For further understanding the function of Fkbp6 in chromosome synapsis, we evaluated chromosome pairings during male meiosis in the as/as rat, a spontaneous null mutation, and compared them with those of the mouse model. Electron microscopy of the pachytene nuclei unveiled several types of abnormal chromosome pairing in the rat model, as shown in the mouse previously. The frequencies of aberrant pairings in the knockout mice and mutant rats were 42 of 67 nuclei (62.7%) and 20 out of 74 nuclei (27.0%), respectively. In order to clarify the mechanism of male specific infertility in Fkbp6 deficiency, the localization of γH2AX, a marker protein of XY chromosome inactivation during male meiosis, was examined. Immunostaining of γH2AX unveiled normal localization of the molecule to XY chromosomes (XY body) in both models, showing the independency of FKBP6 in sex chromosome inactivation. Besides the XY body, focal localization of γH2AX was observed in accordance with the unsynapsed chromosomes in both types of null animal. These results indicate the fundamental role of Fkbp6 in homologous chromosome synapsis during male meiosis. In conclusion, male specific infertility under Fkbp6 deficiency remains unsolved.
KW - Chromosome pairing
KW - FK506 binding protein 6 (FKBP6)
KW - Meiosis
KW - Mouse
KW - Phosphorylated histone H2AX (γH2AX)
KW - Rat
UR - http://www.scopus.com/inward/record.url?scp=46349094871&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=46349094871&partnerID=8YFLogxK
U2 - 10.1262/jrd.19158
DO - 10.1262/jrd.19158
M3 - Article
C2 - 18408354
AN - SCOPUS:46349094871
VL - 54
SP - 203
EP - 207
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 3
ER -