Aeromonas sobria serine protease decreases epithelial Barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro

Hidetomo Kobayashi, Soshi Seike, Masafumi Yamaguchi, Mitsunobu Ueda, Eizo Takahashi, Keinosuke Okamoto, Hiroyasu Yamanaka

Research output: Contribution to journalArticle

Abstract

Aeromonas sobria is a pathogen causing food-borne illness. In immunocompromised patients and the elderly, A. sobria can leave the intestinal tract, and this opportunistically leads to severe extraintestinal diseases including sepsis, peritonitis, and meningitis. To cause such extraintestinal diseases, A. sobria must pass through the intestinal epithelial Barrier. The mechanism of such bacterial translocation has not been established. Herein we used intestinal (T84) cultured cells to investigate the effect of A. sobria serine protease (ASP) on Junctional complexes that maintain the intercellular adhesion of the intestinal epithelium. When several A. sobria strains were inoculated into T84 monolayer grown on Transwell inserts, the strain with higher ASP production largely decreased the value of transepithelial electrical resistance exhibited by the T84 monolayer and markedly caused bacterial translocation from the apical surface into the basolateral side of T84 monolayer. Further experiments revealed that ASP acts on adherens Junctions (AJs) and causes the destruction of both nectin-2 and afadin, which are protein components constituting AJs. Other studies have not revealed the bacterial pathogenic factors that cause the destruction of both nectin-2 and afadin, and our present results thus provide the first report that the bacterial extracellular protease ASP affects these molecules. We speculate that the destruction of nectin-2 and afadin by the action of ASP increases the ability of A. sobria to pass through intestinal epithelial tissue and contributes to the severity of pathological conditions.

Original languageEnglish
Article numbere0221344
JournalPloS one
Volume14
Issue number8
DOIs
Publication statusPublished - Jan 1 2019
Externally publishedYes

Fingerprint

Aeromonas sobria
Bacterial Translocation
Aeromonas
Serine Proteases
serine proteinases
Monolayers
Adherens Junctions
Intestinal Mucosa
cells
Foodborne Diseases
Acoustic impedance
Immunocompromised Host
Pathogens
intestinal mucosa
Peritonitis
Electric Impedance
Meningitis
Cultured Cells
Sepsis
Peptide Hydrolases

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Aeromonas sobria serine protease decreases epithelial Barrier function in T84 cells and accelerates bacterial translocation across the T84 monolayer in vitro. / Kobayashi, Hidetomo; Seike, Soshi; Yamaguchi, Masafumi; Ueda, Mitsunobu; Takahashi, Eizo; Okamoto, Keinosuke; Yamanaka, Hiroyasu.

In: PloS one, Vol. 14, No. 8, e0221344, 01.01.2019.

Research output: Contribution to journalArticle

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