Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-

Akira Takahashi, Masayuki Nakano, Keinosuke Okamoto, Yoshio Fujii, Kazuaki Mawatari, Nagakatsu Harada, Yutaka Nakaya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalFEMS Microbiology Letters
Volume258
Issue number1
DOIs
Publication statusPublished - May 2006

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Keywords

  • Aeromonas sobria hemolysin (ASH)
  • Cystic fibrosis transmembrane conductance regulator (CFTR)
  • HCO secretion
  • Short circuit currents (I)

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

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