Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-

Akira Takahashi, Masayuki Nakano, Keinosuke Okamoto, Yoshio Fujii, Kazuaki Mawatari, Nagakatsu Harada, Yutaka Nakaya

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

Original languageEnglish
Pages (from-to)92-95
Number of pages4
JournalFEMS Microbiology Letters
Volume258
Issue number1
DOIs
Publication statusPublished - May 2006

Fingerprint

Aeromonas
Hemolysin Proteins
Diarrhea
Carbonic Anhydrase Inhibitors
Anions
Virulence Factors
Intestinal Mucosa
Epithelial Cells

Keywords

  • Aeromonas sobria hemolysin (ASH)
  • Cystic fibrosis transmembrane conductance regulator (CFTR)
  • HCO secretion
  • Short circuit currents (I)

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-. / Takahashi, Akira; Nakano, Masayuki; Okamoto, Keinosuke; Fujii, Yoshio; Mawatari, Kazuaki; Harada, Nagakatsu; Nakaya, Yutaka.

In: FEMS Microbiology Letters, Vol. 258, No. 1, 05.2006, p. 92-95.

Research output: Contribution to journalArticle

Takahashi, A, Nakano, M, Okamoto, K, Fujii, Y, Mawatari, K, Harada, N & Nakaya, Y 2006, 'Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-', FEMS Microbiology Letters, vol. 258, no. 1, pp. 92-95. https://doi.org/10.1111/j.1574-6968.2006.00204.x
Takahashi, Akira ; Nakano, Masayuki ; Okamoto, Keinosuke ; Fujii, Yoshio ; Mawatari, Kazuaki ; Harada, Nagakatsu ; Nakaya, Yutaka. / Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-. In: FEMS Microbiology Letters. 2006 ; Vol. 258, No. 1. pp. 92-95.
@article{f0aa91b3b0234c499da1ed7cb17a0275,
title = "Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-",
abstract = "Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.",
keywords = "Aeromonas sobria hemolysin (ASH), Cystic fibrosis transmembrane conductance regulator (CFTR), HCO secretion, Short circuit currents (I)",
author = "Akira Takahashi and Masayuki Nakano and Keinosuke Okamoto and Yoshio Fujii and Kazuaki Mawatari and Nagakatsu Harada and Yutaka Nakaya",
year = "2006",
month = "5",
doi = "10.1111/j.1574-6968.2006.00204.x",
language = "English",
volume = "258",
pages = "92--95",
journal = "FEMS Microbiology Letters",
issn = "0378-1097",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Aeromonas sobria hemolysin causes diarrhea by increasing secretion of HCO3-

AU - Takahashi, Akira

AU - Nakano, Masayuki

AU - Okamoto, Keinosuke

AU - Fujii, Yoshio

AU - Mawatari, Kazuaki

AU - Harada, Nagakatsu

AU - Nakaya, Yutaka

PY - 2006/5

Y1 - 2006/5

N2 - Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

AB - Aeromonas sobria hemolysin (ASH) is one of the major virulence factors produced by A. sobria, a causative agent of diarrhea in humans. We investigated the effects of ASH on anion transport in human colonic epithelial cells. ASH increased short circuit currents across the intestinal epithelia, which were suppressed by anion channel antagonists, such as carbonic anhydrase inhibitors, and by the removal of external HCO3-. Iliac fluid accumulation was also inhibited by carbonic anhydrase inhibitors. The results suggest that ASH activates HCO3- secretion, whose level correlates with the severity of diarrhea.

KW - Aeromonas sobria hemolysin (ASH)

KW - Cystic fibrosis transmembrane conductance regulator (CFTR)

KW - HCO secretion

KW - Short circuit currents (I)

UR - http://www.scopus.com/inward/record.url?scp=33645472799&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33645472799&partnerID=8YFLogxK

U2 - 10.1111/j.1574-6968.2006.00204.x

DO - 10.1111/j.1574-6968.2006.00204.x

M3 - Article

C2 - 16630261

AN - SCOPUS:33645472799

VL - 258

SP - 92

EP - 95

JO - FEMS Microbiology Letters

JF - FEMS Microbiology Letters

SN - 0378-1097

IS - 1

ER -