Aegilops section Sitopsis species contains the introgressive polA1 gene with a closer relationship to that of Hordeum than Triticum-Aegilops species

Ikuo Nakamura, Bhuwan Rai, Hiroko Takahashi, Kenji Kato, Yo ichiro Sato, Takao Komatsuda

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

The section Sitopsis in the genus Aegilops includes five species, Ae. speltoides, Ae. longissima, Ae. sharonensis, Ae. searsii, and Ae. bicornis, which share the SS genome. Although extensive molecular studies have indi- cated Ae. speltoides as a donor of BB or GG genome to polyploid wheat species, the precise relationships among SS, BB, and GG genomes remain unclear. PolA1 is a single-copy nuclear gene encoding the largest subunit of RNA polymerase I. Highly polymorphic PolA1 exon 20 sequences were analyzed for 11 Triticum- Aegilops, 13 Hordeum and three related species. Phylogenetic analyses of the PolA1 gene showed that Triticum-Aegilops and Hordeum species were distinctly separated into two clades. Two related species, Secale cereale and Dasypyrum villosum, were grouped into Triticum and Hordeum clades, respectively. Interestingly, seven accessions of the Sitopsis species were clustered into the Hordeum clade whereas two accessions belonged to the Triticum clade. In contrast, all accessions of Sitopsis species shared the same haplotype of plastid PSID sequences with Triticum-Aegilops species. This inconsistency in phylogeny between nuclear and cytoplasmic sequences suggested that the Sitopsis species probably originated through introgres- sive hybridization between ancestral species of Triticum-Aegilops and Hordeum.

Original languageEnglish
Pages (from-to)602-610
Number of pages9
JournalBreeding Science
Volume59
Issue number5
DOIs
Publication statusPublished - 2009

Fingerprint

Aegilops
Hordeum
Triticum
Genes
genes
Genome
genome
RNA Polymerase I
Plastids
Polyploidy
Dasypyrum villosum
Phylogeny
Haplotypes
phylogeny
Secale cereale
Exons
DNA-directed RNA polymerase
polyploidy
introgression
plastids

Keywords

  • Aegilops
  • Hybrid speciation
  • Introgression
  • Pola1 gene
  • PSID
  • Sitopsis

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Genetics
  • Plant Science

Cite this

Aegilops section Sitopsis species contains the introgressive polA1 gene with a closer relationship to that of Hordeum than Triticum-Aegilops species. / Nakamura, Ikuo; Rai, Bhuwan; Takahashi, Hiroko; Kato, Kenji; Sato, Yo ichiro; Komatsuda, Takao.

In: Breeding Science, Vol. 59, No. 5, 2009, p. 602-610.

Research output: Contribution to journalArticle

Nakamura, Ikuo ; Rai, Bhuwan ; Takahashi, Hiroko ; Kato, Kenji ; Sato, Yo ichiro ; Komatsuda, Takao. / Aegilops section Sitopsis species contains the introgressive polA1 gene with a closer relationship to that of Hordeum than Triticum-Aegilops species. In: Breeding Science. 2009 ; Vol. 59, No. 5. pp. 602-610.
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abstract = "The section Sitopsis in the genus Aegilops includes five species, Ae. speltoides, Ae. longissima, Ae. sharonensis, Ae. searsii, and Ae. bicornis, which share the SS genome. Although extensive molecular studies have indi- cated Ae. speltoides as a donor of BB or GG genome to polyploid wheat species, the precise relationships among SS, BB, and GG genomes remain unclear. PolA1 is a single-copy nuclear gene encoding the largest subunit of RNA polymerase I. Highly polymorphic PolA1 exon 20 sequences were analyzed for 11 Triticum- Aegilops, 13 Hordeum and three related species. Phylogenetic analyses of the PolA1 gene showed that Triticum-Aegilops and Hordeum species were distinctly separated into two clades. Two related species, Secale cereale and Dasypyrum villosum, were grouped into Triticum and Hordeum clades, respectively. Interestingly, seven accessions of the Sitopsis species were clustered into the Hordeum clade whereas two accessions belonged to the Triticum clade. In contrast, all accessions of Sitopsis species shared the same haplotype of plastid PSID sequences with Triticum-Aegilops species. This inconsistency in phylogeny between nuclear and cytoplasmic sequences suggested that the Sitopsis species probably originated through introgres- sive hybridization between ancestral species of Triticum-Aegilops and Hordeum.",
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