@article{30240af6c55d4270968f9fce2bd9333d,
title = "Advances in Structural Biology and the Application to Biological Filament Systems",
abstract = "Structural biology has experienced several transformative technological advances in recent years. These include: development of extremely bright X-ray sources (microfocus synchrotron beamlines and free electron lasers) and the use of electrons to extend protein crystallography to ever decreasing crystal sizes; and an increase in the resolution attainable by cryo-electron microscopy. Here we discuss the use of these techniques in general terms and highlight their application for biological filament systems, an area that is severely underrepresented in atomic resolution structures. We assemble a model of a capped tropomyosin-actin minifilament to demonstrate the utility of combining structures determined by different techniques. Finally, we survey the methods that attempt to transform high resolution structural biology into more physiological environments, such as the cell. Together these techniques promise a compelling decade for structural biology and, more importantly, they will provide exciting discoveries in understanding the designs and purposes of biological machines.",
keywords = "ParM, actin, capping protein, cryo-electron microscopy, filaments, tropomodulin, tropomyosin",
author = "David Popp and Fujiet Koh and Scipion, {Clement P.M.} and Umesh Ghoshdastider and Akihiro Narita and Holmes, {Kenneth C.} and Robinson, {Robert C.}",
note = "Funding Information: Dr. D. Popp, Dr. F. Koh, C. P. M. Scipion, Dr. U. Ghoshdastider, Prof. R. C. Robinson Institute of Molecular and Cell Biology A*STAR (Agency for Science, Technology and Research) Biopolis, Singapore 138673, Singapore E-mail: rrobinson@imcb.a-star.edu.sg C. P. M. Scipion, Prof. R. C. Robinson Department of Biochemistry Yong Loo Lin School of Medicine National University of Singapore Singapore 117597, Singapore Prof A. Narita Nagoya University Graduate School of Science Structural Biology Research Center and Division of Biological Sciences Furo-cho, Chikusa-ku, Nagoya 464-8601, Japan Prof. K. C. Holmes Max Planck Institute for Medical Research D69120 Heidelberg, Germany Prof. R. C. Robinson Research Institute for Interdisciplinary Science Okayama University Okayama 700-8530, Japan {\textcopyright} 2018 The Authors. BioEssays Published by WILEY Periodicals, Inc. This is an open access article under the terms of the Creative Commons Attribution-NonCommercial License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. Publisher Copyright: {\textcopyright} 2018 The Authors. BioEssays Published by WILEY Periodicals, Inc.",
year = "2018",
month = apr,
doi = "10.1002/bies.201700213",
language = "English",
volume = "40",
journal = "BioEssays",
issn = "0265-9247",
publisher = "John Wiley and Sons Inc.",
number = "4",
}