Advances in Structural Biology and the Application to Biological Filament Systems

David Popp, Fujiet Koh, Clement P.M. Scipion, Umesh Ghoshdastider, Akihiro Narita, Kenneth C. Holmes, Robert C. Robinson

Research output: Contribution to journalArticlepeer-review

8 Citations (Scopus)


Structural biology has experienced several transformative technological advances in recent years. These include: development of extremely bright X-ray sources (microfocus synchrotron beamlines and free electron lasers) and the use of electrons to extend protein crystallography to ever decreasing crystal sizes; and an increase in the resolution attainable by cryo-electron microscopy. Here we discuss the use of these techniques in general terms and highlight their application for biological filament systems, an area that is severely underrepresented in atomic resolution structures. We assemble a model of a capped tropomyosin-actin minifilament to demonstrate the utility of combining structures determined by different techniques. Finally, we survey the methods that attempt to transform high resolution structural biology into more physiological environments, such as the cell. Together these techniques promise a compelling decade for structural biology and, more importantly, they will provide exciting discoveries in understanding the designs and purposes of biological machines.

Original languageEnglish
Article number1700213
Issue number4
Publication statusPublished - Apr 2018


  • ParM
  • actin
  • capping protein
  • cryo-electron microscopy
  • filaments
  • tropomodulin
  • tropomyosin

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)


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