TY - JOUR
T1 - Advanced glycation end products enhance monocyte activation during human mixed lymphocyte reaction
AU - Ohashi, Katsuhisa
AU - Takahashi, Hideo Kohka
AU - Mori, Shuji
AU - Liu, Keyue
AU - Wake, Hidenori
AU - Sadamori, Hiroshi
AU - Matsuda, Hiroaki
AU - Yagi, Takahito
AU - Yoshino, Tadashi
AU - Nishibori, Masahiro
AU - Tanaka, Noriaki
N1 - Funding Information:
This work was supported in part by grants from the Japan Society for the Promotion of Science [Grants 18590509 , 20590539 , 17659159 , 19659061 , 21659141 , 21390071 , 215905694 ], from the Scientific Research from Ministry of Health, Labour and Welfare of Japan, and from the Takeda Science Foundation.
PY - 2010/3
Y1 - 2010/3
N2 - Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human mixed lymphocyte reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
AB - Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human mixed lymphocyte reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
KW - Advanced glycation end products
KW - Mixed lymphocyte reaction
KW - Posttransplant diabetes mellitus
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U2 - 10.1016/j.clim.2009.10.008
DO - 10.1016/j.clim.2009.10.008
M3 - Article
C2 - 19914138
AN - SCOPUS:76049128362
VL - 134
SP - 345
EP - 353
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 3
ER -
