Abstract
Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human mixed lymphocyte reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
| Original language | English |
|---|---|
| Pages (from-to) | 345-353 |
| Number of pages | 9 |
| Journal | Clinical Immunology |
| Volume | 134 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Mar 2010 |
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Keywords
- Advanced glycation end products
- Mixed lymphocyte reaction
- Posttransplant diabetes mellitus
ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Advanced glycation end products enhance monocyte activation during human mixed lymphocyte reaction. / Ohashi, Katsuhisa; Takahashi, Hideo Kohka; Mori, Shuji; Liu, Keyue; Wake, Hidenori; Sadamori, Hiroshi; Matsuda, Hiroaki; Yagi, Takahito; Yoshino, Tadashi; Nishibori, Masahiro; Tanaka, Noriaki.
In: Clinical Immunology, Vol. 134, No. 3, 03.2010, p. 345-353.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Advanced glycation end products enhance monocyte activation during human mixed lymphocyte reaction
AU - Ohashi, Katsuhisa
AU - Takahashi, Hideo Kohka
AU - Mori, Shuji
AU - Liu, Keyue
AU - Wake, Hidenori
AU - Sadamori, Hiroshi
AU - Matsuda, Hiroaki
AU - Yagi, Takahito
AU - Yoshino, Tadashi
AU - Nishibori, Masahiro
AU - Tanaka, Noriaki
PY - 2010/3
Y1 - 2010/3
N2 - Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human mixed lymphocyte reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
AB - Posttransplant diabetes mellitus (PTDM) is a frequent complication among transplant recipients. Ligation of advanced glycation end products (AGEs) with their receptor (RAGE) on monocytes/macrophages plays roles in the diabetes complications. The enhancement of adhesion molecule expression on monocytes/macrophages activates T-cells, leading to reduced allograft survival. We investigated the effect of four distinct AGE subtypes (AGE-2/AGE-3/AGE-4/AGE-5) on the expressions of intracellular adhesion molecule (ICAM)-1, B7.1, B7.2 and CD40 on monocytes, the production of interferon (IFN)-γ and tumor necrosis factor (TNF)-α and the proliferation of T-cells during human mixed lymphocyte reaction (MLR). AGE-2 and AGE-3 selectively induced the adhesion molecule expression, cytokine production and T-cell proliferation. The AGE-induced up-regulation of adhesion molecule expression was involved in the cytokine production and T-cell proliferation. AGE-2 and AGE-3 up-regulated the expression of RAGE on monocytes; therefore, the AGEs may activate monocytes, leading to the up-regulation of adhesion molecule expression, cytokine production and T-cell proliferation.
KW - Advanced glycation end products
KW - Mixed lymphocyte reaction
KW - Posttransplant diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=76049128362&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=76049128362&partnerID=8YFLogxK
U2 - 10.1016/j.clim.2009.10.008
DO - 10.1016/j.clim.2009.10.008
M3 - Article
C2 - 19914138
AN - SCOPUS:76049128362
VL - 134
SP - 345
EP - 353
JO - Clinical Immunology
JF - Clinical Immunology
SN - 1521-6616
IS - 3
ER -