Adult human retinal pigment epithelial cells capable of differentiating into neurons

Kaori Amemiya, Masatoshi Haruta, Masayo Takahashi, Mitsuko Kosaka, Goro Eguchi

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

We investigated the ability of adult human RPE cells to differentiate into neurons. Two cell lines were evaluated. The cells were cultured in medium with 8% serum, transferred to a neural stem cell maintenance culture, and induced to differentiate with retinoic acid. The cells were immunocytochemically examined at each step. The cells showed epithelial-like morphology with 8% serum and all were immunoreactive for β-III tubulin. After transfer to the stem cell maintenance culture, they changed morphologically and became immunoreactive for MAP5 and neurofilament200 after inducement with retinoic acid. The ratio of MAP5 positive cells was higher in the young adult RPE cell line. No GFAP or rod-opsin immunoreactive cells were observed. Adult human RPE cells even from old person are capable of differentiating into neurons, although the ratio of mature neurons was greater in the young than in the old cell line in this condition.

Original languageEnglish
Pages (from-to)1-5
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume316
Issue number1
DOIs
Publication statusPublished - Mar 26 2004

Keywords

  • Adult human retinal pigment epithelium cell
  • Differentiation
  • Glia
  • Neuron

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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