Adsorption of proteins derived from fetal bovine serum onto hydroxyapatite nanocrystals with quartz crystal microbalance technique

M. Tagaya, T. Ikoma, T. Takemura, M. Okuda, N. Hanagata, T. Yoshioka, D. Chakarov, B. Kasemo, J. Tanaka

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The adsorption of multiple proteins derived from fetal bovine serum (FBS) in phosphate buffer saline (PBS) and alpha minimum essential (αMEM) was in situ analyzed with a quartz crystal microbalance with dissipation technique on gold, titanium and HAp sensors. The adsorption behaviors of FBS proteins were varied depending on the sensors. The ΔD/Δf value of the HAp sensor were clearly different in PBS and αMEM, and others were not changed. The viscoelastic properties of the protein films adsorbed on the HAp sensor in PBS were flexible in comparison with those on the gold and titanium sensors. The D-f plots incidated that the proteins adsorbed on HAp in PBS would lead to competitive adsorption and conformational change and those in αMEM could form a monolayer. The adsorption behavior on the HAp in carbonate buffer saline was found to be similar to that in αMEM. These differential adsorption behaviors on the HAp surface were attributed to the pre-adsorptive ion, such PO43- or CO32- in the solvent.

Original languageEnglish
Pages (from-to)47-50
Number of pages4
JournalKey Engineering Materials
Volume396-398
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Biosensor
  • Fetal bovine serum (FBS)
  • Hydroxyapatite (HAp)
  • Protein adsorption
  • QCM-D

ASJC Scopus subject areas

  • Materials Science(all)
  • Mechanics of Materials
  • Mechanical Engineering

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    Tagaya, M., Ikoma, T., Takemura, T., Okuda, M., Hanagata, N., Yoshioka, T., Chakarov, D., Kasemo, B., & Tanaka, J. (2009). Adsorption of proteins derived from fetal bovine serum onto hydroxyapatite nanocrystals with quartz crystal microbalance technique. Key Engineering Materials, 396-398, 47-50.