Adsorption characteristics of various proteins to a titanium surface

Koreyoshi Imamura, Miyuki Shimomura, Shungo Nagai, Megumi Akamatsu, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

50 Citations (Scopus)

Abstract

Adsorption characteristics of 18 proteins, with different sizes and isoelectric points, to a titanium oxide surface were studied. The adsorption isotherms were categorized based on protein type and pH: type 1, irreversible adsorption; type 2, Langmuir-type reversible adsorption; and type 3, reversible and irreversible adsorption. Most of the proteins tested were irreversibly adsorbed in the pH range of 3-8, whereas most adsorbed reversibly at pH 8.5-9.4. Protamine, with a pI value of 12, adsorbed reversibly in the pH range of 3-9. pH values that gave maximal sums of irreversibly and reversibly adsorbed proteins were in the pH range of 3-8 and tended to increase slightly with the pI value of the corresponding protein. pH values that gave maximal quantities of irreversibly adsorbed protein ranged between 4-6 and were nearly independent of pI.

Original languageEnglish
Pages (from-to)273-278
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume106
Issue number3
DOIs
Publication statusPublished - Sep 1 2008

Keywords

  • adsorption
  • irreversible adsorption
  • isoelectric point
  • isotherms
  • titanium surface

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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