Adsorption characteristics of tryptic fragments of bovine β- lactoglobulin on a stainless steel surface

Takaharu Sakiyama, Kei Tanino, Masako Urakawa, Koreyoshi Imamura, Tokio Takahashi, Tadashi Nagai, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

40 Citations (Scopus)

Abstract

As a strategy for the analysis of the mode of protein adsorption onto stainless steel surfaces, peptides obtained by tryptic digestion of bovine β-lactoglobulin were subjected to adsorption experiments after identification of their primary structures. In the presence of 1 mM KOH, the peptides were scarcely adsorbed onto the surfaces of stainless steel particles from the peptide mixture. The adsorption experiments on isolated peptides showed that the affinities of the peptides for stainless steel surfaces in the presence of 1 mM HNO3 were significantly different from each other. Peptides without any acidic amino acid residues were scarcely adsorbed onto the surface, whereas some peptides with acidic amino acid residues were found to be irreversibly adsorbed onto the surfaces in the acidic pH region. As for the latter peptides, the amount adsorbed on the surface increased with increasing ionic strength. These results indicated that the carboxyl groups on the side chains of the peptides play an important role in the adsorption. Furthermore, the adsorption behavior of β-lactoglobulin itself was found to be very similar to that of one of the latter peptides.

Original languageEnglish
Pages (from-to)536-541
Number of pages6
JournalJournal of Bioscience and Bioengineering
Volume88
Issue number5
DOIs
Publication statusPublished - 1999

Fingerprint

Lactoglobulins
Stainless Steel
Peptides
Adsorption
Stainless steel
Acidic Amino Acids
Amino acids
Ionic strength
Osmolar Concentration
Digestion
Experiments
Proteins

Keywords

  • β-lactoglobulin
  • Adsorption
  • Peptide
  • Stainless steel surface
  • Tryptic digestion

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering

Cite this

Adsorption characteristics of tryptic fragments of bovine β- lactoglobulin on a stainless steel surface. / Sakiyama, Takaharu; Tanino, Kei; Urakawa, Masako; Imamura, Koreyoshi; Takahashi, Tokio; Nagai, Tadashi; Nakanishi, Kazuhiro.

In: Journal of Bioscience and Bioengineering, Vol. 88, No. 5, 1999, p. 536-541.

Research output: Contribution to journalArticle

Sakiyama, Takaharu ; Tanino, Kei ; Urakawa, Masako ; Imamura, Koreyoshi ; Takahashi, Tokio ; Nagai, Tadashi ; Nakanishi, Kazuhiro. / Adsorption characteristics of tryptic fragments of bovine β- lactoglobulin on a stainless steel surface. In: Journal of Bioscience and Bioengineering. 1999 ; Vol. 88, No. 5. pp. 536-541.
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AU - Takahashi, Tokio

AU - Nagai, Tadashi

AU - Nakanishi, Kazuhiro

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