Adrenomedullin regulates the speed of oviductal fluid flow in cattle

Yuka Yoshimoto, Takumi Nishie, Sayaka Ito, Yoshihiko Kobayashi, Yuki Yamamoto, Kiyoshi Okuda, Koji Kimura

Research output: Contribution to journalArticle

Abstract

Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.

Original languageEnglish
Pages (from-to)712-718
Number of pages7
JournalMolecular Reproduction and Development
Volume84
Issue number8
DOIs
Publication statusPublished - Aug 1 2017

Fingerprint

Adrenomedullin
Calcitonin Receptor-Like Protein
Adrenomedullin Receptors
Receptor Activity-Modifying Protein 3
Receptor Activity-Modifying Protein 2
Epithelial Cells
Follicular Phase
Estrous Cycle
Oviducts
Peptide Hormones
Microspheres
Proteins
Calcium
Pregnancy
Messenger RNA

Keywords

  • ciliary beating
  • epithelial cell
  • intracellular calcium
  • RAMPs

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology
  • Cell Biology

Cite this

Adrenomedullin regulates the speed of oviductal fluid flow in cattle. / Yoshimoto, Yuka; Nishie, Takumi; Ito, Sayaka; Kobayashi, Yoshihiko; Yamamoto, Yuki; Okuda, Kiyoshi; Kimura, Koji.

In: Molecular Reproduction and Development, Vol. 84, No. 8, 01.08.2017, p. 712-718.

Research output: Contribution to journalArticle

Yoshimoto, Yuka ; Nishie, Takumi ; Ito, Sayaka ; Kobayashi, Yoshihiko ; Yamamoto, Yuki ; Okuda, Kiyoshi ; Kimura, Koji. / Adrenomedullin regulates the speed of oviductal fluid flow in cattle. In: Molecular Reproduction and Development. 2017 ; Vol. 84, No. 8. pp. 712-718.
@article{a0c567cf5634488d9b3ff2b38f37ccb1,
title = "Adrenomedullin regulates the speed of oviductal fluid flow in cattle",
abstract = "Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.",
keywords = "ciliary beating, epithelial cell, intracellular calcium, RAMPs",
author = "Yuka Yoshimoto and Takumi Nishie and Sayaka Ito and Yoshihiko Kobayashi and Yuki Yamamoto and Kiyoshi Okuda and Koji Kimura",
year = "2017",
month = "8",
day = "1",
doi = "10.1002/mrd.22852",
language = "English",
volume = "84",
pages = "712--718",
journal = "Molecular Reproduction and Development",
issn = "1040-452X",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Adrenomedullin regulates the speed of oviductal fluid flow in cattle

AU - Yoshimoto, Yuka

AU - Nishie, Takumi

AU - Ito, Sayaka

AU - Kobayashi, Yoshihiko

AU - Yamamoto, Yuki

AU - Okuda, Kiyoshi

AU - Kimura, Koji

PY - 2017/8/1

Y1 - 2017/8/1

N2 - Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.

AB - Unidirectional flow of oviductal fluid from the ovarian to uterine side of the ampulla plays a significant role in successful pregnancy, and is produced by ciliary beating. Various systems regulate ciliary beating, such as paracrine, autocrine, and endocrine. We hypothesized that Adrenomedullin (ADM)—a peptide hormone that acts via its receptors, which are complexes of Calcitonin receptor-like receptor (CRLR) and Receptor activity-modifying protein (RAMP) 2 or 3 — promotes oviductal fluid flow in the ampulla of bovine oviducts. First, we examined the expression of ADM, CRLR, RAMP2, and RAMP3 mRNAs in isolated epithelial cells throughout the estrous cycle, and the localization of ADM receptor protein constituents in the ampulla. RAMP2 expression was significantly higher in the follicular phase. Furthermore, RAMP2 protein was detected only in ciliated cells, whereas CRLR and RAMP3 were detected in all epithelial cells. The effects of ADM and an ADM antagonist on fluid-flow speed were examined using microbeads in ampullary tissue. ADM antagonist decreased bead transport speed, and this decrease was reversed by ADM. In addition, ADM recovered the bead transport speed that decreased in the absence of calcium. Overall, our results suggest that ADM contributes to the regulation of oviductal fluid flow in ampulla.

KW - ciliary beating

KW - epithelial cell

KW - intracellular calcium

KW - RAMPs

UR - http://www.scopus.com/inward/record.url?scp=85026362083&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85026362083&partnerID=8YFLogxK

U2 - 10.1002/mrd.22852

DO - 10.1002/mrd.22852

M3 - Article

C2 - 28621496

AN - SCOPUS:85026362083

VL - 84

SP - 712

EP - 718

JO - Molecular Reproduction and Development

JF - Molecular Reproduction and Development

SN - 1040-452X

IS - 8

ER -