Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery

Hinako Shiraki, Hiromu Kawasaki, Satoko Tezuka, Akira Nakatsuma, Hideki Nawa, Hiroaki Araki, Yutaka Gomita, Yuji Kurosaki

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Mechanisms underlying acetylcholine-induced endothelium-independent vasodilation were studied in the rat mesenteric vascular bed isolated from Wistar rats. In preparations without endothelium, and contracted by perfusion with Krebs solution containing methoxamine (2-7 μM), perfusion of acetylcholine (1-100 μM) for 1 min produced a concentration-dependent vasodilation. Denervation of denuded preparations by cold storage (4°C for 72 h) abolished the acetylcholine-induced vasodilation; 10 and 100 nM atropine abolished 1 and 10 μM acetylcholine-induced vasodilation, but it inhibited only 20% of vasodilation by 100 μM acetylcholine. The acetylcholine-induced atropine-resistant vasodilation was inhibited by 10 and 100 μM hexamethonium, 5 μM guanethidine, 50 μM bretylium, in vitro 6-hydroxydopamine (2 mM for 20 min, twice), 1 μM capsaicin and 0.5 μM calcitonin gene-related peptide (CGRP)-(8-37) (CGRP receptor antagonist). These findings suggest that the acetylcholine-induced endothelium-independent nicotinic vasodilation requires the presence of intact adrenergic nerves, and is mediated by endogenous CGRP released from CGRP-containing nerves.

Original languageEnglish
Pages (from-to)231-242
Number of pages12
JournalEuropean Journal of Pharmacology
Volume419
Issue number2-3
DOIs
Publication statusPublished - May 11 2001

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Mesenteric Arteries
Vasodilation
Adrenergic Agents
Acetylcholine
Endothelium
Calcitonin Gene-Related Peptide
Atropine
Perfusion
Calcitonin Gene-Related Peptide Receptors
Methoxamine
Guanethidine
Hexamethonium
Oxidopamine
Capsaicin
Denervation
Blood Vessels
Wistar Rats

Keywords

  • Acetylcholine-induced
  • Adrenergic nerves
  • CGRP-containing nerve
  • Endothelium-independent
  • Nicotinic acetylcholine receptor
  • Vasodilation

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Pharmacology

Cite this

Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery. / Shiraki, Hinako; Kawasaki, Hiromu; Tezuka, Satoko; Nakatsuma, Akira; Nawa, Hideki; Araki, Hiroaki; Gomita, Yutaka; Kurosaki, Yuji.

In: European Journal of Pharmacology, Vol. 419, No. 2-3, 11.05.2001, p. 231-242.

Research output: Contribution to journalArticle

Shiraki, Hinako ; Kawasaki, Hiromu ; Tezuka, Satoko ; Nakatsuma, Akira ; Nawa, Hideki ; Araki, Hiroaki ; Gomita, Yutaka ; Kurosaki, Yuji. / Adrenergic nerves mediate acetylcholine-induced endothelium-independent vasodilation in the rat mesenteric resistance artery. In: European Journal of Pharmacology. 2001 ; Vol. 419, No. 2-3. pp. 231-242.
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