Abstract
Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.
| Original language | English |
|---|---|
| Pages (from-to) | 265-272 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 167 |
| Issue number | 1 |
| DOIs | |
| Publication status | Published - Feb 28 1990 |
| Externally published | Yes |
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ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells. / Nishiki, Tei-ichi; Narumiya, Shuh; Morii, Narito; Yamamoto, Masamitsu; Fujiwara, Motohatsu; Kamata, Yoichi; Sakaguchi, Genji; Kozaki, Shunji.
In: Biochemical and Biophysical Research Communications, Vol. 167, No. 1, 28.02.1990, p. 265-272.Research output: Contribution to journal › Article
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TY - JOUR
T1 - ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells
AU - Nishiki, Tei-ichi
AU - Narumiya, Shuh
AU - Morii, Narito
AU - Yamamoto, Masamitsu
AU - Fujiwara, Motohatsu
AU - Kamata, Yoichi
AU - Sakaguchi, Genji
AU - Kozaki, Shunji
PY - 1990/2/28
Y1 - 1990/2/28
N2 - Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.
AB - Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.
UR - http://www.scopus.com/inward/record.url?scp=0025233512&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0025233512&partnerID=8YFLogxK
U2 - 10.1016/0006-291X(90)91760-P
DO - 10.1016/0006-291X(90)91760-P
M3 - Article
VL - 167
SP - 265
EP - 272
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 1
ER -