ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells

Tei-ichi Nishiki, Shuh Narumiya, Narito Morii, Masamitsu Yamamoto, Motohatsu Fujiwara, Yoichi Kamata, Genji Sakaguchi, Shunji Kozaki

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Botulinum ADP-ribosyltransferase C3 (C3 exoenzyme) was purified to homogeneity and added to cultured rat pheochromocytoma PC-12 cells. Incubation with this exoenzyme caused inhibition of cell growth and induced neurites as well as acetylcholine esterase in these cells. These changes were dependent on the amount of the enzyme added to the culture, which correlated with the in situ ADP-ribosylation of the rho rac proteins in the cells. Preincubation with a specific anti-C3 exoenzyme monoclonal antibody inhibited both the ADP-ribosyl-transferase activity and the neurite-inducing activity of the enzyme preparation. These results suggest that C3 exoenzyme affected the cellular function of the rho rac proteins by ADP-ribosylation to induce these changes in the cells.

Original languageEnglish
Pages (from-to)265-272
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume167
Issue number1
DOIs
Publication statusPublished - Feb 28 1990
Externally publishedYes

Fingerprint

rac GTP-Binding Proteins
Esterases
Adenosine Diphosphate
Acetylcholine
Growth
ADP Ribose Transferases
Neurites
Cell growth
Enzymes
Transferases
Rats
Monoclonal Antibodies
Pheochromocytoma
Neuronal Outgrowth

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells. / Nishiki, Tei-ichi; Narumiya, Shuh; Morii, Narito; Yamamoto, Masamitsu; Fujiwara, Motohatsu; Kamata, Yoichi; Sakaguchi, Genji; Kozaki, Shunji.

In: Biochemical and Biophysical Research Communications, Vol. 167, No. 1, 28.02.1990, p. 265-272.

Research output: Contribution to journalArticle

Nishiki, Tei-ichi ; Narumiya, Shuh ; Morii, Narito ; Yamamoto, Masamitsu ; Fujiwara, Motohatsu ; Kamata, Yoichi ; Sakaguchi, Genji ; Kozaki, Shunji. / ADP-ribosylation of the rho rac proteins induces growth inhibition, neurite outgrowth and acetylcholine esterase in cultured PC-12 cells. In: Biochemical and Biophysical Research Communications. 1990 ; Vol. 167, No. 1. pp. 265-272.
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