Administration of granulocyte colony-stimulating factor induces hyporesponsiveness to lipopolysaccharide and impairs antigen-presenting function of peripheral blood monocytes

Kazutaka Sunami, Takanori Teshima, Yuichiro Nawa, Yasushi Hiramatsu, Yoshinobu Maeda, Katsuto Takenaka, Katsuji Shinagawa, Fumihiko Ishimaru, Kazuma Ikeda, Kenji Niiya, Mine Harada

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Objective. The incidence and severity of acute graft-vs-host disease after allogeneic transplantation of granulocyte colony-stimulating factor (G-CSF)-mobilized peripheral blood stem cells (PBSC) are not greater than those after conventional bone marrow transplantation despite infusion of more than one log greater number of donor T cells in PBSC. It has been postulated that monocytes from G-CSF-mobilized donors suppress alloreactivity of donor T cells. Materials and Methods. We investigated the phenotype and function of monocytes in normal individuals receiving 10 μg/kg of G-CSF for 4 days. Results. Monocytes were phenotypically and functionally different after G-CSF administration from steady-state monocytes. They were characterized by an increased CD14+CD16+ subpopulation, reduced expression of HLA-DR, and diminished ability to produce tumor necrosis factor-α and interleukin-10 to lipopolysaccharide, compared with steady-state monocytes. These alterations were not replicated by culturing monocytes with G-CSF in vitro, suggesting an indirect effect of G-CSF. In addition, the antigen-presenting function of G-CSF-mobilized monocytes was impaired. Conclusion. Hyporesponsiveness of G-CSF-treated monocytes to lipopolysaccharide with regard to tumor necrosis factor-α production, together with impaired antigen-presenting function, may be responsible for the unexpectedly low incidence of graft-vs-host disease after G-CSF-mobilized PBSC transplantation.

Original languageEnglish
Pages (from-to)1117-1124
Number of pages8
JournalExperimental Hematology
Volume29
Issue number9
DOIs
Publication statusPublished - Jan 1 2001

ASJC Scopus subject areas

  • Molecular Biology
  • Hematology
  • Genetics
  • Cell Biology
  • Cancer Research

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