Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials

Yoshiki Nakashima, Chika Miyagi-Shiohira, Naoya Kobayashi, Issei Saitoh, Masami Watanabe, Hirofumi Noguchi

Research output: Contribution to journalArticle

Abstract

Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95%) and exocrine tissue (purity ≥99%) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5% FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.

Original languageEnglish
Article numbere1460294
JournalIslets
Volume10
Issue number3
DOIs
Publication statusPublished - May 4 2018

Fingerprint

Islets of Langerhans
Swine
Fibronectins
Adhesives
Laminin
Bovine Serum Albumin
Collagen Type I
Fibrinogen
Tissue Adhesions
Islets of Langerhans Transplantation
Heterologous Transplantation
Collagen Type IV
Extracellular Matrix
Serum

Keywords

  • cell adhesion
  • collagen
  • exocrine tissue
  • fibronectin
  • integrin
  • islet
  • pancreas
  • porcine

ASJC Scopus subject areas

  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

Cite this

Nakashima, Y., Miyagi-Shiohira, C., Kobayashi, N., Saitoh, I., Watanabe, M., & Noguchi, H. (2018). Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials. Islets, 10(3), [e1460294]. https://doi.org/10.1080/19382014.2018.1460294

Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials. / Nakashima, Yoshiki; Miyagi-Shiohira, Chika; Kobayashi, Naoya; Saitoh, Issei; Watanabe, Masami; Noguchi, Hirofumi.

In: Islets, Vol. 10, No. 3, e1460294, 04.05.2018.

Research output: Contribution to journalArticle

Nakashima, Y, Miyagi-Shiohira, C, Kobayashi, N, Saitoh, I, Watanabe, M & Noguchi, H 2018, 'Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials', Islets, vol. 10, no. 3, e1460294. https://doi.org/10.1080/19382014.2018.1460294
Nakashima, Yoshiki ; Miyagi-Shiohira, Chika ; Kobayashi, Naoya ; Saitoh, Issei ; Watanabe, Masami ; Noguchi, Hirofumi. / Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials. In: Islets. 2018 ; Vol. 10, No. 3.
@article{bbaa5715ea454940af08e04ca979521e,
title = "Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials",
abstract = "Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95{\%}) and exocrine tissue (purity ≥99{\%}) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5{\%} FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.",
keywords = "cell adhesion, collagen, exocrine tissue, fibronectin, integrin, islet, pancreas, porcine",
author = "Yoshiki Nakashima and Chika Miyagi-Shiohira and Naoya Kobayashi and Issei Saitoh and Masami Watanabe and Hirofumi Noguchi",
year = "2018",
month = "5",
day = "4",
doi = "10.1080/19382014.2018.1460294",
language = "English",
volume = "10",
journal = "Islets",
issn = "1938-2014",
publisher = "Landes Bioscience",
number = "3",

}

TY - JOUR

T1 - Adhesion characteristics of porcine pancreatic islets and exocrine tissue to coating materials

AU - Nakashima, Yoshiki

AU - Miyagi-Shiohira, Chika

AU - Kobayashi, Naoya

AU - Saitoh, Issei

AU - Watanabe, Masami

AU - Noguchi, Hirofumi

PY - 2018/5/4

Y1 - 2018/5/4

N2 - Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95%) and exocrine tissue (purity ≥99%) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5% FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.

AB - Since the report of the Edmonton protocol in 2000, islet transplantation has been implemented worldwide, and xenotransplantation using porcine islets has also been reported. In addition, many basic experiments using pancreatic islets and exocrine tissue after isolation have been reported. Recently, exocrine cells have been found to be essential for inducing the differentiation of pancreatic islets. Therefore, the importance of the culture conditions for pancreatic tissue when conducting experiments using pancreatic tissue is also increasing. In this study, we focused on the coat material and examined the adhesive properties of porcine pancreatic islets and exocrine tissue after isolation. Porcine islet isolation was performed, and isolated islets (purity ≥95%) and exocrine tissue (purity ≥99%) were used to achieve adhesion to several extracellular matrixes, fibronectin, collagen type I, collagen type IV, laminin I, fibrinogen, and bovine serum albumin (BSA). DMEM with 0.5% FBS was used as the assay medium. For exocrine tissue, the adhesion was promoted in fibronectin, collagen type I, laminin I, and fibrinogen. The adhesive ability to fibronectin was more than twice that to BSA, while the adhesive ability to collagen type I, laminin I, and fibrinogen was less than twice that to BSA. For islets, the adhesive ability to fibronectin was weaker than that of exocrine tissue. Furthermore, the adhesion effect in fibronectin was obtained within 30 minutes and in medium containing little serum for both islets and exocrine tissues. These data suggest that fibronectin may be useful for the adhesion of pancreatic tissue.

KW - cell adhesion

KW - collagen

KW - exocrine tissue

KW - fibronectin

KW - integrin

KW - islet

KW - pancreas

KW - porcine

UR - http://www.scopus.com/inward/record.url?scp=85047987783&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047987783&partnerID=8YFLogxK

U2 - 10.1080/19382014.2018.1460294

DO - 10.1080/19382014.2018.1460294

M3 - Article

VL - 10

JO - Islets

JF - Islets

SN - 1938-2014

IS - 3

M1 - e1460294

ER -