Adenovirus mediated gene delivery to the joints of guinea pigs

Takumi Ikeda, Toshikazu Kubo, Yuji Arai, Tohru Nakanishi, Kappei Kobayashi, Kenji Takahashi, Jiro Imanishi, Masaharu Takigawa, Yasusuke Hirasawa

Research output: Contribution to journalArticle

68 Citations (Scopus)

Abstract

Objective. To clarify in vivo applicability of adenovirus mediated gene delivery to examine a gene therapy for human joint diseases. Methods. We directly injected vectors harbouring β-galactosidase gene and transforming growth factor (TGF)-β1 gene into the joints of Hartley guinea pigs. Expressions of delivered LacZ were examined by 5-bromo-4-chloro-3-indolyl- β-D-galactoside staining and reverse transcription-polymerase chain reaction. The levels of TGF-β1 that were delivered to the joint and then transferred to the joint fluid were assessed by ELISA. Results. LacZ expression was observed in almost all synovial tissue samples and in chondrocytes on the surface of degenerated cartilage. In the other organs, expression of delivered genes was not observed. For 2 weeks following gene delivery TGF-β1 levels in joint fluid were significantly higher than the levels in the controls for 2 weeks. Conclusion. Direct gene delivery into the joint cavity is feasible with the in vivo gene delivery method using adenovirus vector and would be clinically applicable.

Original languageEnglish
Pages (from-to)1666-1673
Number of pages8
JournalJournal of Rheumatology
Volume25
Issue number9
Publication statusPublished - Sep 1998

Keywords

  • Adenovirus vector
  • Gene therapy
  • Osteoarthritis

ASJC Scopus subject areas

  • Rheumatology
  • Immunology and Allergy
  • Immunology

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  • Cite this

    Ikeda, T., Kubo, T., Arai, Y., Nakanishi, T., Kobayashi, K., Takahashi, K., Imanishi, J., Takigawa, M., & Hirasawa, Y. (1998). Adenovirus mediated gene delivery to the joints of guinea pigs. Journal of Rheumatology, 25(9), 1666-1673.