Adenoviral targeting of malignant melanoma for fluorescenceguided surgery prevents recurrence in orthotopic nude-mouse models

Shuya Yano, Kiyoto Takehara, Hiroyuki Kishimoto, Yasuo Urata, Shunsuke Kagawa, Michael Bouvet, Toshiyoshi Fujiwara, Robert M. Hoffman

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Malignant melanoma requires precise resection in order to avoid metastatic recurrence. We report here that the telomerase-dependent, green fluorescent protein (GFP)-containing adenovirus OBP-401 could label malignant melanoma with GFP in situ in orthotopic mouse models. OBP-401-based fluorescence-guided surgery (FGS) resulted in the complete resection of malignant melanoma in the orthotopic models, where conventional bright-light surgery (BLS) could not. High-dose administration of OBP-401 enabled FGS without residual cancer cells or recurrence, due to its dual effect of cancer-cell labeling with GFP and killing.

Original languageEnglish
Pages (from-to)18558-18572
Number of pages15
JournalOncotarget
Volume7
Issue number14
DOIs
Publication statusPublished - Jan 1 2016

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Keywords

  • Adenovirus
  • Fluorescence-guided surgery (FGS)
  • Melanoma
  • Nude mice
  • OBP-401

ASJC Scopus subject areas

  • Oncology

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