Adenoviral-mediated overexpression of monocyte chemoattractant protein-1 differentially alters the development of Th1 and Th2 type responses in vivo

Akihiro Matsukawa, Nicholas W. Lukacs, Theodore J. Standiford, Stephen W. Chensue, Steven L. Kunkel

Research output: Contribution to journalArticle

45 Citations (Scopus)

Abstract

The expression of chemokines during an immune response may participate in determining the intensity and type of the developing immune response. In the present study, we have examined the effect of overexpressing monocyte chemoattractant Protein (MCP)-1 at the site of immunization during different stages of Th1- and Th2-type granulomatous responses. The overexpression of MCP-1 by MCP-1 adenovirus during the sensitization phase of the purified protein derivative Th1-type model significantly reduced the elicitation of the granulomatous response. In contrast, the overexpression of MCP-1 during the sensitization phase of the schistosome egg Ag Th2 response led to an enhanced granulomatous reaction. When cytokines were examined upon restimulation of splenocytes ex vivo, an altered cytokine profile was observed, as compared with control mice. IFN-γ and IL-12 were significantly reduced in the purified Protein derivative Th1-type response, whereas IL-10 and IL-13 were up-regulated in the schistosome egg Ag Th2-type response. The regulation of the immune response was further examined by using the MCP-1 adenovirus at later time points during the elicitation phase. When MCP-1 was overexpressed during the elicitation phase of the responses, neither the Th1- type nor the Th2-type granuloma was altered. Likewise, the cytokine profiles after restimulation of splenocytes ex vivo were unchanged. Thus, the function of MCP-1 may depend on the stage and type of immune response.

Original languageEnglish
Pages (from-to)1699-1704
Number of pages6
JournalJournal of Immunology
Volume164
Issue number4
Publication statusPublished - Feb 15 2000
Externally publishedYes

Fingerprint

Chemokine CCL2
Cytokines
Adenoviridae
Ovum
Interleukin-13
Interleukin-12
Granuloma
Chemokines
Interleukin-10
Immunization
Proteins

ASJC Scopus subject areas

  • Immunology

Cite this

Adenoviral-mediated overexpression of monocyte chemoattractant protein-1 differentially alters the development of Th1 and Th2 type responses in vivo. / Matsukawa, Akihiro; Lukacs, Nicholas W.; Standiford, Theodore J.; Chensue, Stephen W.; Kunkel, Steven L.

In: Journal of Immunology, Vol. 164, No. 4, 15.02.2000, p. 1699-1704.

Research output: Contribution to journalArticle

Matsukawa, Akihiro ; Lukacs, Nicholas W. ; Standiford, Theodore J. ; Chensue, Stephen W. ; Kunkel, Steven L. / Adenoviral-mediated overexpression of monocyte chemoattractant protein-1 differentially alters the development of Th1 and Th2 type responses in vivo. In: Journal of Immunology. 2000 ; Vol. 164, No. 4. pp. 1699-1704.
@article{0a23e8cb5e924174b70437e54f13e51e,
title = "Adenoviral-mediated overexpression of monocyte chemoattractant protein-1 differentially alters the development of Th1 and Th2 type responses in vivo",
abstract = "The expression of chemokines during an immune response may participate in determining the intensity and type of the developing immune response. In the present study, we have examined the effect of overexpressing monocyte chemoattractant Protein (MCP)-1 at the site of immunization during different stages of Th1- and Th2-type granulomatous responses. The overexpression of MCP-1 by MCP-1 adenovirus during the sensitization phase of the purified protein derivative Th1-type model significantly reduced the elicitation of the granulomatous response. In contrast, the overexpression of MCP-1 during the sensitization phase of the schistosome egg Ag Th2 response led to an enhanced granulomatous reaction. When cytokines were examined upon restimulation of splenocytes ex vivo, an altered cytokine profile was observed, as compared with control mice. IFN-γ and IL-12 were significantly reduced in the purified Protein derivative Th1-type response, whereas IL-10 and IL-13 were up-regulated in the schistosome egg Ag Th2-type response. The regulation of the immune response was further examined by using the MCP-1 adenovirus at later time points during the elicitation phase. When MCP-1 was overexpressed during the elicitation phase of the responses, neither the Th1- type nor the Th2-type granuloma was altered. Likewise, the cytokine profiles after restimulation of splenocytes ex vivo were unchanged. Thus, the function of MCP-1 may depend on the stage and type of immune response.",
author = "Akihiro Matsukawa and Lukacs, {Nicholas W.} and Standiford, {Theodore J.} and Chensue, {Stephen W.} and Kunkel, {Steven L.}",
year = "2000",
month = "2",
day = "15",
language = "English",
volume = "164",
pages = "1699--1704",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Adenoviral-mediated overexpression of monocyte chemoattractant protein-1 differentially alters the development of Th1 and Th2 type responses in vivo

AU - Matsukawa, Akihiro

AU - Lukacs, Nicholas W.

AU - Standiford, Theodore J.

AU - Chensue, Stephen W.

AU - Kunkel, Steven L.

PY - 2000/2/15

Y1 - 2000/2/15

N2 - The expression of chemokines during an immune response may participate in determining the intensity and type of the developing immune response. In the present study, we have examined the effect of overexpressing monocyte chemoattractant Protein (MCP)-1 at the site of immunization during different stages of Th1- and Th2-type granulomatous responses. The overexpression of MCP-1 by MCP-1 adenovirus during the sensitization phase of the purified protein derivative Th1-type model significantly reduced the elicitation of the granulomatous response. In contrast, the overexpression of MCP-1 during the sensitization phase of the schistosome egg Ag Th2 response led to an enhanced granulomatous reaction. When cytokines were examined upon restimulation of splenocytes ex vivo, an altered cytokine profile was observed, as compared with control mice. IFN-γ and IL-12 were significantly reduced in the purified Protein derivative Th1-type response, whereas IL-10 and IL-13 were up-regulated in the schistosome egg Ag Th2-type response. The regulation of the immune response was further examined by using the MCP-1 adenovirus at later time points during the elicitation phase. When MCP-1 was overexpressed during the elicitation phase of the responses, neither the Th1- type nor the Th2-type granuloma was altered. Likewise, the cytokine profiles after restimulation of splenocytes ex vivo were unchanged. Thus, the function of MCP-1 may depend on the stage and type of immune response.

AB - The expression of chemokines during an immune response may participate in determining the intensity and type of the developing immune response. In the present study, we have examined the effect of overexpressing monocyte chemoattractant Protein (MCP)-1 at the site of immunization during different stages of Th1- and Th2-type granulomatous responses. The overexpression of MCP-1 by MCP-1 adenovirus during the sensitization phase of the purified protein derivative Th1-type model significantly reduced the elicitation of the granulomatous response. In contrast, the overexpression of MCP-1 during the sensitization phase of the schistosome egg Ag Th2 response led to an enhanced granulomatous reaction. When cytokines were examined upon restimulation of splenocytes ex vivo, an altered cytokine profile was observed, as compared with control mice. IFN-γ and IL-12 were significantly reduced in the purified Protein derivative Th1-type response, whereas IL-10 and IL-13 were up-regulated in the schistosome egg Ag Th2-type response. The regulation of the immune response was further examined by using the MCP-1 adenovirus at later time points during the elicitation phase. When MCP-1 was overexpressed during the elicitation phase of the responses, neither the Th1- type nor the Th2-type granuloma was altered. Likewise, the cytokine profiles after restimulation of splenocytes ex vivo were unchanged. Thus, the function of MCP-1 may depend on the stage and type of immune response.

UR - http://www.scopus.com/inward/record.url?scp=0034651652&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0034651652&partnerID=8YFLogxK

M3 - Article

C2 - 10657613

AN - SCOPUS:0034651652

VL - 164

SP - 1699

EP - 1704

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -