Adenoviral Bak overexpression mediates caspase-dependent tumor killing

Abujiang Pataer, Bingliang Fang, Robert Yu, Shunsuke Kagawa, Kelly K. Hunt, Timothy J. McDonnell, Jack A. Roth, Stephen G. Swisher

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

One of the most promising strategies in cancer gene therapy is adenoviral transfer of proapoptotic genes. We therefore evaluated the novel strategy of adenovirally overexpressing the proapoptotic Bak gene. Our results showed marked apoptosis in cancer cells in vivo and in vitro after Bak gene transfer via a binary adenoviral vector system. This effect was not seen in a caspase 3-defective cell line (MCF-7) and was abrogated in Bak- sensitive tumors after administration of the caspase inhibitor zDEVD-fmk. Our results suggest that adenoviral-mediated overexpression of Bak provides a novel therapeutic strategy for cancer therapy, but this process appears to be caspase dependent.

Original languageEnglish
Pages (from-to)788-792
Number of pages5
JournalCancer Research
Volume60
Issue number4
Publication statusPublished - Feb 15 2000
Externally publishedYes

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Caspases
Genes
Neoplasms
Caspase Inhibitors
Neoplasm Genes
Caspase 3
Genetic Therapy
Apoptosis
Cell Line
Therapeutics

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Pataer, A., Fang, B., Yu, R., Kagawa, S., Hunt, K. K., McDonnell, T. J., ... Swisher, S. G. (2000). Adenoviral Bak overexpression mediates caspase-dependent tumor killing. Cancer Research, 60(4), 788-792.

Adenoviral Bak overexpression mediates caspase-dependent tumor killing. / Pataer, Abujiang; Fang, Bingliang; Yu, Robert; Kagawa, Shunsuke; Hunt, Kelly K.; McDonnell, Timothy J.; Roth, Jack A.; Swisher, Stephen G.

In: Cancer Research, Vol. 60, No. 4, 15.02.2000, p. 788-792.

Research output: Contribution to journalArticle

Pataer, A, Fang, B, Yu, R, Kagawa, S, Hunt, KK, McDonnell, TJ, Roth, JA & Swisher, SG 2000, 'Adenoviral Bak overexpression mediates caspase-dependent tumor killing', Cancer Research, vol. 60, no. 4, pp. 788-792.
Pataer A, Fang B, Yu R, Kagawa S, Hunt KK, McDonnell TJ et al. Adenoviral Bak overexpression mediates caspase-dependent tumor killing. Cancer Research. 2000 Feb 15;60(4):788-792.
Pataer, Abujiang ; Fang, Bingliang ; Yu, Robert ; Kagawa, Shunsuke ; Hunt, Kelly K. ; McDonnell, Timothy J. ; Roth, Jack A. ; Swisher, Stephen G. / Adenoviral Bak overexpression mediates caspase-dependent tumor killing. In: Cancer Research. 2000 ; Vol. 60, No. 4. pp. 788-792.
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