Adenosine stimulates neuromedin U mRNA expression in the rat pars tuberalis

Sayaka Aizawa, Tingting Gu, A. Kaminoda, R. Fujioka, Fumiya Ojima, Ichiro Sakata, Takafumi Sakai, Maho Ogoshi, S. Takahashi, S. Takeuchi

Research output: Contribution to journalArticle

Abstract

Neuromedin U (NMU) shows circadian expression in the rat pars tuberalis (PT), and is known to be suppressed by melatonin. Here we examined the involvement of adenosine in the regulation of Nmu expression. We found that the rat PT expressed adenosine receptor A2b and that an adenosine receptor agonist, NECA, stimulated Nmu expression in brain slice cultures. In vitro promoter assays revealed that NECA stimulated Nmu promoter activity via a cAMP response element (CRE) in the presence of adenosine receptor A2b. NECA also increased the levels of phosphorylated CRE-binding protein. These findings suggest that adenosine stimulates Nmu expression by activating the cAMP signaling pathway through adenosine receptor A2b in the rat PT. This is the first report to demonstrate that Nmu expression in the PT is regulated by adenosine, which acts as an intravital central metabolic signal, in addition to melatonin, which acts as an external photoperiodic environmental signal.

Original languageEnglish
Article number110518
JournalMolecular and cellular endocrinology
Volume496
DOIs
Publication statusPublished - Oct 1 2019

Keywords

  • Adenosine receptor
  • Circadian rhythm
  • Melatonin
  • Neuromedin U
  • Pars tuberalis
  • cAMP response element

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Endocrinology

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