TY - JOUR
T1 - Addition of metal chlorides to a HOCl conditioner can enhance bond strength to smear layer deproteinized dentin
AU - Sanon, Kittisak
AU - Tichy, Antonin
AU - Hatayama, Takashi
AU - Thanatvarakorn, Ornnicha
AU - Prasansuttiporn, Taweesak
AU - Wada, Takahiro
AU - Shimada, Yasushi
AU - Hosaka, Keiichi
AU - Nakajima, Masatoshi
N1 - Funding Information:
This work was funded by the Ministry of Education, Culture, Sports, Science and Technology of Japan (grant numbers 18K09571 and 19K10106 ). Kittisak Sanon was funded by the Integrated Development in Innovation and Technology for Geriatric Oral Health Care Project , Faculty of Dentistry, Chulalongkorn University , Bangkok, Thailand. Antonin Tichy was supported by Charles University, Prague, Czech Republic (grant Cooperatio 207030 Dental Medicine/LF1 ). Taweesak Prasansuttiporn was supported by Chiang Mai University, Thailand.
Publisher Copyright:
© 2022 Elsevier Inc.
PY - 2022
Y1 - 2022
N2 - Objectives: To evaluate the effect of smear layer deproteinization using hypochlorous acid (HOCl) with/without metal chlorides (SrCl2 and ZnCl2) on the microtensile bond strength (µTBS) of two simplified adhesives to dentin. Methods: Human dentin surfaces with a standardized smear layer were pretreated using a 105 ppm HOCl solution with/without SrCl2 (0.05 M, 0.1 M, 0.2 M, 0.4 M) or ZnCl2 (0.05 M, 0.1 M, 0.2 M) for 5 s, 15 s, or 30 s. After the deproteinizing solution was washed out with water for 5 s, 15 s, or 30 s, pretreated surfaces were bonded with one-step self-etch adhesive Bond Force II or universal adhesive Clearfil Universal Bond Quick, and µTBS was measured after 24 h. Additionally, the deproteinizing effects of HOCl solutions with/without the metal chlorides were compared by measuring changes in the amide:phosphate ratio using attenuated total reflection Fourier transform infrared spectroscopy. Statistical analysis was performed using multifactor ANOVA, Tukey's post hoc tests and t-tests (p < 0.05). Results: Pretreatment with pure HOCl for 15 s and 30 s significantly decreased the amide:phosphate ratio (p < 0.05), indicating effective deproteinization, but the µTBS of both adhesives increased significantly only if HOCl was washed out for 30 s (p < 0.05). Increasing the concentrations of metal chlorides enabled shortening of the wash-out time down to 5 s while maintaining the improved µTBS (p < 0.05). The deproteinizing effect of HOCl was not significantly altered by the addition of metal chlorides (p > 0.05).
AB - Objectives: To evaluate the effect of smear layer deproteinization using hypochlorous acid (HOCl) with/without metal chlorides (SrCl2 and ZnCl2) on the microtensile bond strength (µTBS) of two simplified adhesives to dentin. Methods: Human dentin surfaces with a standardized smear layer were pretreated using a 105 ppm HOCl solution with/without SrCl2 (0.05 M, 0.1 M, 0.2 M, 0.4 M) or ZnCl2 (0.05 M, 0.1 M, 0.2 M) for 5 s, 15 s, or 30 s. After the deproteinizing solution was washed out with water for 5 s, 15 s, or 30 s, pretreated surfaces were bonded with one-step self-etch adhesive Bond Force II or universal adhesive Clearfil Universal Bond Quick, and µTBS was measured after 24 h. Additionally, the deproteinizing effects of HOCl solutions with/without the metal chlorides were compared by measuring changes in the amide:phosphate ratio using attenuated total reflection Fourier transform infrared spectroscopy. Statistical analysis was performed using multifactor ANOVA, Tukey's post hoc tests and t-tests (p < 0.05). Results: Pretreatment with pure HOCl for 15 s and 30 s significantly decreased the amide:phosphate ratio (p < 0.05), indicating effective deproteinization, but the µTBS of both adhesives increased significantly only if HOCl was washed out for 30 s (p < 0.05). Increasing the concentrations of metal chlorides enabled shortening of the wash-out time down to 5 s while maintaining the improved µTBS (p < 0.05). The deproteinizing effect of HOCl was not significantly altered by the addition of metal chlorides (p > 0.05).
KW - Adhesion
KW - FTIR
KW - Hypochlorous acid
KW - Microtensile bond strength
KW - Pretreatment
KW - Smear layer deproteinization
KW - Strontium chloride
KW - Zinc chloride
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U2 - 10.1016/j.dental.2022.06.006
DO - 10.1016/j.dental.2022.06.006
M3 - Article
AN - SCOPUS:85132540803
JO - Dental Materials
JF - Dental Materials
SN - 0109-5641
ER -