ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

Kadir Demircan, Tomoko Yonezawa, Tomoyuki Takigawa, Vehap Topcu, Serpil Erdogan, Fatma Ucar, Ferah Armutcu, M. Ramazan Yigitoglu, Yoshifumi Ninomiya, Satoshi Hirohata

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.

Original languageEnglish
Pages (from-to)25-30
Number of pages6
JournalNeuroscience Letters
Volume544
DOIs
Publication statusPublished - Jun 7 2013

Fingerprint

Proteoglycans
Spinal Cord Injuries
Chondroitin Sulfate Proteoglycans
Brain
Central Nervous System
Thrombospondins
Disintegrins
Biological Phenomena
Neuronal Plasticity
Metalloproteases
Neuroglia
Astrocytes
Arthritis
Cartilage
Cicatrix
Extracellular Matrix
Peptide Hydrolases
Western Blotting
Immunohistochemistry
Polymerase Chain Reaction

Keywords

  • ADAMTS
  • Aggrecanase
  • Astrocytes
  • Spinal cord injury

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse. / Demircan, Kadir; Yonezawa, Tomoko; Takigawa, Tomoyuki; Topcu, Vehap; Erdogan, Serpil; Ucar, Fatma; Armutcu, Ferah; Yigitoglu, M. Ramazan; Ninomiya, Yoshifumi; Hirohata, Satoshi.

In: Neuroscience Letters, Vol. 544, 07.06.2013, p. 25-30.

Research output: Contribution to journalArticle

Demircan, Kadir ; Yonezawa, Tomoko ; Takigawa, Tomoyuki ; Topcu, Vehap ; Erdogan, Serpil ; Ucar, Fatma ; Armutcu, Ferah ; Yigitoglu, M. Ramazan ; Ninomiya, Yoshifumi ; Hirohata, Satoshi. / ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse. In: Neuroscience Letters. 2013 ; Vol. 544. pp. 25-30.
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