ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

Kadir Demircan; Tomoko Yonezawa; Tomoyuki Takigawa; Vehap Topcu; Serpil Erdogan; Fatma Ucar; Ferah Armutcu; M. Ramazan Yigitoglu; Yoshifumi Ninomiya; Satoshi Hirohata

doi:10.1016/j.neulet.2013.02.064

ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

Kadir Demircan, Tomoko Yonezawa, Tomoyuki Takigawa, Vehap Topcu, Serpil Erdogan, Fatma Ucar, Ferah Armutcu, M. Ramazan Yigitoglu, Yoshifumi Ninomiya, Satoshi Hirohata

Research output: Contribution to journal › Article › peer-review

39 Citations (Scopus)

Abstract

ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.

Original language	English
Pages (from-to)	25-30
Number of pages	6
Journal	Neuroscience Letters
Volume	544
DOIs	https://doi.org/10.1016/j.neulet.2013.02.064
Publication status	Published - Jun 7 2013

Keywords

ADAMTS
Aggrecanase
Astrocytes
Spinal cord injury

ASJC Scopus subject areas

Neuroscience(all)

UN SDGs

This output contributes to the following UN Sustainable Development Goals (SDGs)

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10.1016/j.neulet.2013.02.064

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@article{3f5274c21de3435b905422033cceaa6a,

title = "ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse",

abstract = "ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.",

keywords = "ADAMTS, Aggrecanase, Astrocytes, Spinal cord injury",

author = "Kadir Demircan and Tomoko Yonezawa and Tomoyuki Takigawa and Vehap Topcu and Serpil Erdogan and Fatma Ucar and Ferah Armutcu and Yigitoglu, {M. Ramazan} and Yoshifumi Ninomiya and Satoshi Hirohata",

note = "Funding Information: We acknowledge the support of the Turgut Ozal University and Okayama University Scientific Research Program. K. Demircan is funded by the The Scientific and Technological Research Council of Turkey (T{\"U}BİTAK) grant SBAG 3501 . K.D. received Japan Society of Promotion Science (JSPS) and Takeda Science Foundation Post Doctoral Research Scholarship, respectively. We also thank Dr. Suneel Apte (Cleveland, pioneering scientist of ADAMTS) for their excellent mentoring, encouragement of junior scientists and technical help.",

year = "2013",

month = jun,

day = "7",

doi = "10.1016/j.neulet.2013.02.064",

language = "English",

volume = "544",

pages = "25--30",

journal = "Neuroscience Letters",

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T1 - ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

AU - Demircan, Kadir

AU - Yonezawa, Tomoko

AU - Takigawa, Tomoyuki

AU - Topcu, Vehap

AU - Erdogan, Serpil

AU - Ucar, Fatma

AU - Armutcu, Ferah

AU - Yigitoglu, M. Ramazan

AU - Ninomiya, Yoshifumi

AU - Hirohata, Satoshi

N1 - Funding Information: We acknowledge the support of the Turgut Ozal University and Okayama University Scientific Research Program. K. Demircan is funded by the The Scientific and Technological Research Council of Turkey (TÜBİTAK) grant SBAG 3501 . K.D. received Japan Society of Promotion Science (JSPS) and Takeda Science Foundation Post Doctoral Research Scholarship, respectively. We also thank Dr. Suneel Apte (Cleveland, pioneering scientist of ADAMTS) for their excellent mentoring, encouragement of junior scientists and technical help.

PY - 2013/6/7

Y1 - 2013/6/7

N2 - ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.

AB - ADAMTS (a disintegrin and metalloproteinase with thrombospondin motifs) proteinases are involved in a variety of biological processes such as angiogenesis, cancer and arthritis. ADAMTSs appears to be responsible for the cleavage of proteoglycans in several tissues including brain and cartilage. Chondroitin sulfate proteoglycans (CSPGs) maintains the integrity of the brain extracellular matrix and major inhibitory contributors for glial scar and neural plasticity. The activity of aggrecanases in the central nervous system (CNS) has been reported. ADAMTSs are an enzyme degrading CSPGs in the brain. However, there is a little knowledge regarding ADAMTSs in the CNS. We investigated the expression levels of ADAMTSs mRNAs by RT-PCR after spinal cord injury in mouse. Transcripts encoding 4 of the 19 known ADAMTSs were evaluated in the mouse spinal cord following injury. ADAMTS1, -5 and -9 expression levels were found to be upregulated. No change was observed in ADAMTS4 expression. By means of immunohistochemistry, ADAMTSs were detected in the astrocytes implying its cellular source in SCI. Western blot analyses indicated that aggrecanase-generated proteoglycan fragments are produced after SCI.

KW - ADAMTS

KW - Aggrecanase

KW - Astrocytes

KW - Spinal cord injury

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SN - 0304-3940

VL - 544

SP - 25

EP - 30

JO - Neuroscience Letters

JF - Neuroscience Letters

ER -

ADAMTS1, ADAMTS5, ADAMTS9 and aggrecanase-generated proteoglycan fragments are induced following spinal cord injury in mouse

Abstract

Keywords

ASJC Scopus subject areas

UN SDGs

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