Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs

Min Kyi, Yasunari Miyazaki, Tooru Inoue, Shuji Miyake, Akihiro Matsukawa, Yasuyuki Yoshizawa

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The aim of this study was to assess the acute effects of cigarette smoke exposure on cellular and cytokine profile in BAL fluids in an isolated perfused rabbit assay. The experimental animals were categorized into four groups: (1) unexposed controls and (2) cigarette smoke-exposed animals perfused with autologous whole blood; (3) unexposed controls and cigarette smoke-exposed; (4) cigarette smoke-exposed animals perfused with Krebs' Ringer solution containing 5% bovine serum albumin and glucose. Cigarette smoke induced an increase in total cell numbers (mainly alveolar macrophages in BAL fluids) and an increase in the permeability index of BAL. Levels of interleukin 8 were also significantly decreased in BAL fluids due to acute effects of cigarette smoke exposure. The most likely explanation for cigarette smoke-induced increase of inflammatory cells in BAL in lungs is because of the release of pre-existing cells from reservoirs within the lungs. The acute effects of cigarette smoke-induced increase of pulmonary epithelial permeability may also play an important role in the cellular recruitment into airspaces from the lung reservoirs. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)143-151
Number of pages9
JournalRespiration Physiology
Volume123
Issue number1-2
DOIs
Publication statusPublished - Oct 2000
Externally publishedYes

Fingerprint

Smoke
Tobacco Products
Dimercaprol
Cytokines
Lung
Permeability
Alveolar Macrophages
Bovine Serum Albumin
Interleukin-8
Cell Count
Rabbits
Glucose

Keywords

  • Broncho-alveolar lavage
  • Cells
  • Cigarette
  • Cigarette smoke
  • Cytokines
  • Mammals
  • Pro-inflammatory
  • Rabbit
  • Smoke

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine

Cite this

Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs. / Kyi, Min; Miyazaki, Yasunari; Inoue, Tooru; Miyake, Shuji; Matsukawa, Akihiro; Yoshizawa, Yasuyuki.

In: Respiration Physiology, Vol. 123, No. 1-2, 10.2000, p. 143-151.

Research output: Contribution to journalArticle

Kyi, Min ; Miyazaki, Yasunari ; Inoue, Tooru ; Miyake, Shuji ; Matsukawa, Akihiro ; Yoshizawa, Yasuyuki. / Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs. In: Respiration Physiology. 2000 ; Vol. 123, No. 1-2. pp. 143-151.
@article{8aaa857180da4e199b2a7ecaa18dc14a,
title = "Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs",
abstract = "The aim of this study was to assess the acute effects of cigarette smoke exposure on cellular and cytokine profile in BAL fluids in an isolated perfused rabbit assay. The experimental animals were categorized into four groups: (1) unexposed controls and (2) cigarette smoke-exposed animals perfused with autologous whole blood; (3) unexposed controls and cigarette smoke-exposed; (4) cigarette smoke-exposed animals perfused with Krebs' Ringer solution containing 5{\%} bovine serum albumin and glucose. Cigarette smoke induced an increase in total cell numbers (mainly alveolar macrophages in BAL fluids) and an increase in the permeability index of BAL. Levels of interleukin 8 were also significantly decreased in BAL fluids due to acute effects of cigarette smoke exposure. The most likely explanation for cigarette smoke-induced increase of inflammatory cells in BAL in lungs is because of the release of pre-existing cells from reservoirs within the lungs. The acute effects of cigarette smoke-induced increase of pulmonary epithelial permeability may also play an important role in the cellular recruitment into airspaces from the lung reservoirs. (C) 2000 Elsevier Science B.V.",
keywords = "Broncho-alveolar lavage, Cells, Cigarette, Cigarette smoke, Cytokines, Mammals, Pro-inflammatory, Rabbit, Smoke",
author = "Min Kyi and Yasunari Miyazaki and Tooru Inoue and Shuji Miyake and Akihiro Matsukawa and Yasuyuki Yoshizawa",
year = "2000",
month = "10",
doi = "10.1016/S0034-5687(00)00147-X",
language = "English",
volume = "123",
pages = "143--151",
journal = "Respiratory Physiology and Neurobiology",
issn = "1569-9048",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Acute effects of smoke exposure on the cellular and cytokine profile in isolated perfused lungs

AU - Kyi, Min

AU - Miyazaki, Yasunari

AU - Inoue, Tooru

AU - Miyake, Shuji

AU - Matsukawa, Akihiro

AU - Yoshizawa, Yasuyuki

PY - 2000/10

Y1 - 2000/10

N2 - The aim of this study was to assess the acute effects of cigarette smoke exposure on cellular and cytokine profile in BAL fluids in an isolated perfused rabbit assay. The experimental animals were categorized into four groups: (1) unexposed controls and (2) cigarette smoke-exposed animals perfused with autologous whole blood; (3) unexposed controls and cigarette smoke-exposed; (4) cigarette smoke-exposed animals perfused with Krebs' Ringer solution containing 5% bovine serum albumin and glucose. Cigarette smoke induced an increase in total cell numbers (mainly alveolar macrophages in BAL fluids) and an increase in the permeability index of BAL. Levels of interleukin 8 were also significantly decreased in BAL fluids due to acute effects of cigarette smoke exposure. The most likely explanation for cigarette smoke-induced increase of inflammatory cells in BAL in lungs is because of the release of pre-existing cells from reservoirs within the lungs. The acute effects of cigarette smoke-induced increase of pulmonary epithelial permeability may also play an important role in the cellular recruitment into airspaces from the lung reservoirs. (C) 2000 Elsevier Science B.V.

AB - The aim of this study was to assess the acute effects of cigarette smoke exposure on cellular and cytokine profile in BAL fluids in an isolated perfused rabbit assay. The experimental animals were categorized into four groups: (1) unexposed controls and (2) cigarette smoke-exposed animals perfused with autologous whole blood; (3) unexposed controls and cigarette smoke-exposed; (4) cigarette smoke-exposed animals perfused with Krebs' Ringer solution containing 5% bovine serum albumin and glucose. Cigarette smoke induced an increase in total cell numbers (mainly alveolar macrophages in BAL fluids) and an increase in the permeability index of BAL. Levels of interleukin 8 were also significantly decreased in BAL fluids due to acute effects of cigarette smoke exposure. The most likely explanation for cigarette smoke-induced increase of inflammatory cells in BAL in lungs is because of the release of pre-existing cells from reservoirs within the lungs. The acute effects of cigarette smoke-induced increase of pulmonary epithelial permeability may also play an important role in the cellular recruitment into airspaces from the lung reservoirs. (C) 2000 Elsevier Science B.V.

KW - Broncho-alveolar lavage

KW - Cells

KW - Cigarette

KW - Cigarette smoke

KW - Cytokines

KW - Mammals

KW - Pro-inflammatory

KW - Rabbit

KW - Smoke

UR - http://www.scopus.com/inward/record.url?scp=0033800027&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033800027&partnerID=8YFLogxK

U2 - 10.1016/S0034-5687(00)00147-X

DO - 10.1016/S0034-5687(00)00147-X

M3 - Article

VL - 123

SP - 143

EP - 151

JO - Respiratory Physiology and Neurobiology

JF - Respiratory Physiology and Neurobiology

SN - 1569-9048

IS - 1-2

ER -