Abstract
To examine whether oxygen (O2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO2) during prostaglandin F2α (PGF2α)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF2α analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF2α induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO2 was significantly higher in OV blood than in JV blood (P2α injection increased pO2 in OV blood between 0.5 and 2 h. These results demonstrate that PGF2α induced an acute increase in pO2 and NO in the ovarian circulation and suggest that O2 and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.
| Original language | English |
|---|---|
| Pages (from-to) | 149-155 |
| Number of pages | 7 |
| Journal | Journal of Reproduction and Development |
| Volume | 55 |
| Issue number | 2 |
| DOIs | |
| Publication status | Published - May 13 2009 |
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Keywords
- Cattle
- Luteolysis
- Nitric oxide
- Oxygen
- Prostaglandin F
ASJC Scopus subject areas
- Animal Science and Zoology
Cite this
Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2α-induced luteolysis in cattle. / Acosta, Tomas Javier; Bah, Mamadou Bousa; Korzekwa, Anna; Woclawek-Potocka, Izabela; Markiewicz, Wlodzimierz; Jaroszewski, Jerzy Jan; Okuda, Kiyoshi; Skarzynski, Dariusz Jan.
In: Journal of Reproduction and Development, Vol. 55, No. 2, 13.05.2009, p. 149-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2α-induced luteolysis in cattle
AU - Acosta, Tomas Javier
AU - Bah, Mamadou Bousa
AU - Korzekwa, Anna
AU - Woclawek-Potocka, Izabela
AU - Markiewicz, Wlodzimierz
AU - Jaroszewski, Jerzy Jan
AU - Okuda, Kiyoshi
AU - Skarzynski, Dariusz Jan
PY - 2009/5/13
Y1 - 2009/5/13
N2 - To examine whether oxygen (O2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO2) during prostaglandin F2α (PGF2α)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF2α analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF2α induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO2 was significantly higher in OV blood than in JV blood (P2α injection increased pO2 in OV blood between 0.5 and 2 h. These results demonstrate that PGF2α induced an acute increase in pO2 and NO in the ovarian circulation and suggest that O2 and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.
AB - To examine whether oxygen (O2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO2) during prostaglandin F2α (PGF2α)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF2α analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF2α induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO2 was significantly higher in OV blood than in JV blood (P2α injection increased pO2 in OV blood between 0.5 and 2 h. These results demonstrate that PGF2α induced an acute increase in pO2 and NO in the ovarian circulation and suggest that O2 and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.
KW - Cattle
KW - Luteolysis
KW - Nitric oxide
KW - Oxygen
KW - Prostaglandin F
UR - http://www.scopus.com/inward/record.url?scp=66949167788&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=66949167788&partnerID=8YFLogxK
U2 - 10.1262/jrd.20133
DO - 10.1262/jrd.20133
M3 - Article
C2 - 19106483
AN - SCOPUS:66949167788
VL - 55
SP - 149
EP - 155
JO - Journal of Reproduction and Development
JF - Journal of Reproduction and Development
SN - 0916-8818
IS - 2
ER -