Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2α-induced luteolysis in cattle

Tomas Javier Acosta, Mamadou Bousa Bah, Anna Korzekwa, Izabela Woclawek-Potocka, Wlodzimierz Markiewicz, Jerzy Jan Jaroszewski, Kiyoshi Okuda, Dariusz Jan Skarzynski

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

To examine whether oxygen (O2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO2) during prostaglandin F (PGF)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO2 was significantly higher in OV blood than in JV blood (P2α injection increased pO2 in OV blood between 0.5 and 2 h. These results demonstrate that PGF induced an acute increase in pO2 and NO in the ovarian circulation and suggest that O2 and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.

Original languageEnglish
Pages (from-to)149-155
Number of pages7
JournalJournal of Reproduction and Development
Volume55
Issue number2
DOIs
Publication statusPublished - May 13 2009

Fingerprint

luteolysis
prostaglandins
nitric oxide
progesterone
jugular vein
oxygen
blood veins
cattle
blood
aorta
injection
cows
corpus luteum
catheters
estrous cycle
sodium chloride
nitrites
estrus
nitrates
secretion

Keywords

  • Cattle
  • Luteolysis
  • Nitric oxide
  • Oxygen
  • Prostaglandin F

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2α-induced luteolysis in cattle. / Acosta, Tomas Javier; Bah, Mamadou Bousa; Korzekwa, Anna; Woclawek-Potocka, Izabela; Markiewicz, Wlodzimierz; Jaroszewski, Jerzy Jan; Okuda, Kiyoshi; Skarzynski, Dariusz Jan.

In: Journal of Reproduction and Development, Vol. 55, No. 2, 13.05.2009, p. 149-155.

Research output: Contribution to journalArticle

Acosta, Tomas Javier ; Bah, Mamadou Bousa ; Korzekwa, Anna ; Woclawek-Potocka, Izabela ; Markiewicz, Wlodzimierz ; Jaroszewski, Jerzy Jan ; Okuda, Kiyoshi ; Skarzynski, Dariusz Jan. / Acute changes in circulating concentrations of progesterone and nitric oxide and partial pressure of oxygen during prostaglandin F2α-induced luteolysis in cattle. In: Journal of Reproduction and Development. 2009 ; Vol. 55, No. 2. pp. 149-155.
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AU - Bah, Mamadou Bousa

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AU - Woclawek-Potocka, Izabela

AU - Markiewicz, Wlodzimierz

AU - Jaroszewski, Jerzy Jan

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AU - Skarzynski, Dariusz Jan

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AB - To examine whether oxygen (O2) and nitric oxide (NO) are temporally associated with the acute changes in luteal function during luteolysis, we determined the real-time changes in the circulating concentrations of progesterone (P4) and nitrite/nitrate (the stable metabolites of NO) and the partial pressure of oxygen (pO2) during prostaglandin F2α (PGF2α)-induced luteolysis in cattle. Catheters for frequent blood sample collection were inserted into the ovarian vein (OV), jugular vein (JV) and aorta abdominalis (AA) in 12 cows on Day 9 of the oestrous cycle (oestrus=Day 0). On Day 10, the cows were randomly divided into two groups and treated with a luteolytic dose of a PGF2α analogue or saline solution (control). Blood samples were collected at -2, -1, 0, 0.25, 0.5, 0.75, 1 and 2 h and then at 2-h intervals until 12 h after treatment (0 h). Injection of a PGF2α induced a significant decrease in the concentrations of P4 in OV plasma within 2 h. The decrease in P4 concentrations was preceded by an increase in the NO concentrations in the blood collected from OV, JV and AA. Basal pO2 was significantly higher in OV blood than in JV blood (P2α injection increased pO2 in OV blood between 0.5 and 2 h. These results demonstrate that PGF2α induced an acute increase in pO2 and NO in the ovarian circulation and suggest that O2 and NO are involved in the early events of CL regression, including inhibition of P4 secretion and output, in cattle.

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KW - Nitric oxide

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