Actual substrate for elemental sulfur oxidation by sulfur: ferric ion oxidoreductase purified from Thiobacillus ferrooxidans

Tsuyoshi Sugio, Takayuki Katagiri, Kenji Inagaki, Tatsuo Tano

Research output: Contribution to journalArticle

48 Citations (Scopus)

Abstract

Initial step of elemental sulfur (S0) oxidation by a purified sulfur:ferric ion oxidoreductase from Thiobacillus ferrooxidans was investigated. When S0 and reduced glutathione (GSH), whichwas absolutely required for S0 oxidation by sulfur:ferric ion oxidoreductase, were incubated in a buffer solution (pH 6.5), hydrogen sulfide (H 2S) and GSSG were chemically produced at the rate of 0.021 and 0.082 μmol/ml per h, respectively. If sulfur:ferric ion oxidoreductase was addedto the incubation mixture, H2S production immediately stopped and sulfite production opened, suggesting that H2S is an actual substrate of sulfu:ferric ion oxidoreductase. Amongthe reduced sulfur compounds tested, S0, H2S and FeS were utilized as an electron donorof sulfur:ferric ion oxidoreductase and a mechanism of initial steps of S0 oxidation was proposed. It was also found that when S0 was oxidized by sulfur:ferric ion oxidoreductase in the presence of GSH, contact of sulfur:ferric ion oxidoreductase with solid element sulfur was unnecessary.

Original languageEnglish
Pages (from-to)250-256
Number of pages7
JournalBiochimica et Biophysica Acta - Bioenergetics
Volume973
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Thiobacillus
Sulfur
Oxidation
Substrates
Sulfur Compounds
Hydrogen Sulfide
Sulfites
Glutathione Disulfide
Glutathione
sulfur-ferric ion oxidoreductase
Buffers
Oxidoreductases
Electrons
Ions

Keywords

  • (T. ferrooxidans)
  • Leaching, bacterial
  • Sulfur oxidation
  • Sulfur:ferric ion oxidoreductase

ASJC Scopus subject areas

  • Biophysics

Cite this

Actual substrate for elemental sulfur oxidation by sulfur : ferric ion oxidoreductase purified from Thiobacillus ferrooxidans. / Sugio, Tsuyoshi; Katagiri, Takayuki; Inagaki, Kenji; Tano, Tatsuo.

In: Biochimica et Biophysica Acta - Bioenergetics, Vol. 973, No. 2, 1989, p. 250-256.

Research output: Contribution to journalArticle

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