TY - JOUR
T1 - ACTTS3 encoding a polyketide synthase is essential for the biosynthesis of ACT-Toxin and pathogenicity in the tangerine pathotype of alternaria alternata
AU - Miyamoto, Y.
AU - Masunaka, A.
AU - Tsuge, T.
AU - Yamamoto, M.
AU - Ohtani, K.
AU - Fukumoto, T.
AU - Gomi, K.
AU - Peever, T. L.
AU - Tada, Y.
AU - Ichimura, K.
AU - Akimitsu, K.
N1 - Copyright:
Copyright 2010 Elsevier B.V., All rights reserved.
PY - 2010/4
Y1 - 2010/4
N2 - The tangerine pathotype of Alternaria alternala produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerine and tangerine hybrids. Sequence analy-sis of a genomic BAC clone identified part of the ACT-toxin TOX (ACTT) gene cluster, and knockout experiments have implicated several open reading frames (ORF) contained within the cluster in the biosynthesis of ACT-toxin. One of the ORF, designated ACTTS3, encoding a putative poly-ketide synthase, was isolated by rapid amplification of cDNA ends and genomic/reverse transcription-polymerase chain reactions using the specific primers designed from the BAC sequences. The 7,374-bp ORF encodes a polyketide synthase with putative ß-ketoacyl synthase, acyltransferase, methyltransferase, ß-ketoacyl reductase, and phosphopante-theine attachment site domains. Genomic Southern blots demonstrated that ACTTS3 is present on the smallest chro-mosome in the tangerine pathotype of A. alternata, and the presence of ACTTS3 is highly correlated with ACT-toxin production and pathogenicity. Targeted gene disruption of two copies of ACTTS3 led to a complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS3 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
AB - The tangerine pathotype of Alternaria alternala produces host-selective ACT-toxin and causes Alternaria brown spot disease of tangerine and tangerine hybrids. Sequence analy-sis of a genomic BAC clone identified part of the ACT-toxin TOX (ACTT) gene cluster, and knockout experiments have implicated several open reading frames (ORF) contained within the cluster in the biosynthesis of ACT-toxin. One of the ORF, designated ACTTS3, encoding a putative poly-ketide synthase, was isolated by rapid amplification of cDNA ends and genomic/reverse transcription-polymerase chain reactions using the specific primers designed from the BAC sequences. The 7,374-bp ORF encodes a polyketide synthase with putative ß-ketoacyl synthase, acyltransferase, methyltransferase, ß-ketoacyl reductase, and phosphopante-theine attachment site domains. Genomic Southern blots demonstrated that ACTTS3 is present on the smallest chro-mosome in the tangerine pathotype of A. alternata, and the presence of ACTTS3 is highly correlated with ACT-toxin production and pathogenicity. Targeted gene disruption of two copies of ACTTS3 led to a complete loss of ACT-toxin production and pathogenicity. These results indicate that ACTTS3 is an essential gene for ACT-toxin biosynthesis in the tangerine pathotype of A. alternata and is required for pathogenicity of this fungus.
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U2 - 10.1094/MPMI-23-4-0406
DO - 10.1094/MPMI-23-4-0406
M3 - Article
C2 - 20192828
AN - SCOPUS:77949285013
SN - 0894-0282
VL - 23
SP - 406
EP - 414
JO - Molecular Plant-Microbe Interactions
JF - Molecular Plant-Microbe Interactions
IS - 4
ER -