Activity of nucleotide excision repair enzymes for oxanine cross-link lesions.

Toshiaki Nakano, Atsushi Katafuchi, Hiroaki Terato, Toshinori Suzuki, Bennett Van Houten, Hiroshi Ide

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Nitric oxide and nitrous acid induce deamination of DNA bases, resulting in uracil, hypoxanthine, xanthine, and oxanine (Oxa) as major damage. Oxa reacts further with polyamines and DNA binding proteins, generating bulky cross-link adducts. Recently we have shown Oxa and cross-link adducts are potentially genotoxic lesions. In the present study, we have assessed the role of base excision repair (BER) and nucleotide excision repair (NER) systems in the repair of Oxa and Oxa-spermine (Oxa-Sp) cross-link adducts. Oxa was very poorly removed from DNA by both BER glycosylases and NER enzymes, whereas Oxa-Sp was efficiently excised by E. coli and human NER enzymes.

Original languageEnglish
Pages (from-to)293-294
Number of pages2
JournalNucleic acids symposium series (2004)
Issue number49
Publication statusPublished - Dec 1 2005
Externally publishedYes

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DNA Repair
Enzymes
Nitrous Acid
Nitric Acid
Deamination
Hypoxanthine
Xanthine
Spermine
Uracil
oxanine
DNA
DNA-Binding Proteins
Polyamines
Nitric Oxide
Escherichia coli

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Nakano, T., Katafuchi, A., Terato, H., Suzuki, T., Van Houten, B., & Ide, H. (2005). Activity of nucleotide excision repair enzymes for oxanine cross-link lesions. Nucleic acids symposium series (2004), (49), 293-294.

Activity of nucleotide excision repair enzymes for oxanine cross-link lesions. / Nakano, Toshiaki; Katafuchi, Atsushi; Terato, Hiroaki; Suzuki, Toshinori; Van Houten, Bennett; Ide, Hiroshi.

In: Nucleic acids symposium series (2004), No. 49, 01.12.2005, p. 293-294.

Research output: Contribution to journalArticle

Nakano, T, Katafuchi, A, Terato, H, Suzuki, T, Van Houten, B & Ide, H 2005, 'Activity of nucleotide excision repair enzymes for oxanine cross-link lesions.', Nucleic acids symposium series (2004), no. 49, pp. 293-294.
Nakano, Toshiaki ; Katafuchi, Atsushi ; Terato, Hiroaki ; Suzuki, Toshinori ; Van Houten, Bennett ; Ide, Hiroshi. / Activity of nucleotide excision repair enzymes for oxanine cross-link lesions. In: Nucleic acids symposium series (2004). 2005 ; No. 49. pp. 293-294.
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