Activation of the heterotrimeric G protein α-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis

Lingang Zhang, Qing Wei, Wenjuan Wu, Yuxiang Cheng, Guangzhen Hu, Fenhong Hu, Yi Sun, Ying Zhu, Wataru Sakamoto, Jirong Huang

    Research output: Contribution to journalArticlepeer-review

    58 Citations (Scopus)

    Abstract

    Heterotrimeric G protein knock-out mutants have no phenotypic defect in chloroplast development, and the connection between the G protein signaling pathway and chloroplast development has only been inferred from pharmaceutical evidence. Thus, whether G protein signaling plays a role in chloroplast development remains an open question. Here, we present genetic evidence, using the leaf-variegated mutant thylakoid formation 1 (thf1), indicating that inactivation or activation of the endogenous G protein α-subunit (GPA1) affects chloroplast development, as does the ectopic expression of the constitutively active Gα-subunit (cGPA1). Molecular biological and genetic analyses showed that FtsH complexes, which are composed of type-A (FtsH1/FtsH5) and type-B (FtsH2/FtsH8) subunits, are required for cGPA1-promoted chloroplast development in thf1. Furthermore, the ectopic expression of cGPA1 rescues the leaf variegation of ftsh2. Consistent with this finding, microarray analysis shows that ectopic expression of cGPA1 partially corrects mis-regulated gene expression in thf1. This overlooked function of G proteins provides new insight into our understanding of the integrative signaling network, which dynamically regulates chloroplast development and function in response to both intracellular and extracellular signals.

    Original languageEnglish
    Pages (from-to)1041-1053
    Number of pages13
    JournalPlant Journal
    Volume58
    Issue number6
    DOIs
    Publication statusPublished - Jun 2009

    Keywords

    • Arabidopsis
    • Chloroplast development
    • FtsH protease
    • Heterotrimeric G proteins
    • Variegation

    ASJC Scopus subject areas

    • Genetics
    • Plant Science
    • Cell Biology

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