Activation of the heterotrimeric G protein α-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis

Lingang Zhang, Qing Wei, Wenjuan Wu, Yuxiang Cheng, Guangzhen Hu, Fenhong Hu, Yi Sun, Ying Zhu, Wataru Sakamoto, Jirong Huang

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Heterotrimeric G protein knock-out mutants have no phenotypic defect in chloroplast development, and the connection between the G protein signaling pathway and chloroplast development has only been inferred from pharmaceutical evidence. Thus, whether G protein signaling plays a role in chloroplast development remains an open question. Here, we present genetic evidence, using the leaf-variegated mutant thylakoid formation 1 (thf1), indicating that inactivation or activation of the endogenous G protein α-subunit (GPA1) affects chloroplast development, as does the ectopic expression of the constitutively active Gα-subunit (cGPA1). Molecular biological and genetic analyses showed that FtsH complexes, which are composed of type-A (FtsH1/FtsH5) and type-B (FtsH2/FtsH8) subunits, are required for cGPA1-promoted chloroplast development in thf1. Furthermore, the ectopic expression of cGPA1 rescues the leaf variegation of ftsh2. Consistent with this finding, microarray analysis shows that ectopic expression of cGPA1 partially corrects mis-regulated gene expression in thf1. This overlooked function of G proteins provides new insight into our understanding of the integrative signaling network, which dynamically regulates chloroplast development and function in response to both intracellular and extracellular signals.

Original languageEnglish
Pages (from-to)1041-1053
Number of pages13
JournalPlant Journal
Volume58
Issue number6
DOIs
Publication statusPublished - Jun 2009

Fingerprint

Heterotrimeric GTP-Binding Proteins
Protein Subunits
protein subunits
Chloroplasts
G-proteins
Arabidopsis
chloroplasts
GTP-Binding Proteins
Thylakoids
thylakoids
knockout mutants
Microarray Analysis
leaves
Molecular Biology
inactivation
Gene Expression
drugs
gene expression
mutants
Pharmaceutical Preparations

Keywords

  • Arabidopsis
  • Chloroplast development
  • FtsH protease
  • Heterotrimeric G proteins
  • Variegation

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology
  • Genetics

Cite this

Activation of the heterotrimeric G protein α-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis. / Zhang, Lingang; Wei, Qing; Wu, Wenjuan; Cheng, Yuxiang; Hu, Guangzhen; Hu, Fenhong; Sun, Yi; Zhu, Ying; Sakamoto, Wataru; Huang, Jirong.

In: Plant Journal, Vol. 58, No. 6, 06.2009, p. 1041-1053.

Research output: Contribution to journalArticle

Zhang, Lingang ; Wei, Qing ; Wu, Wenjuan ; Cheng, Yuxiang ; Hu, Guangzhen ; Hu, Fenhong ; Sun, Yi ; Zhu, Ying ; Sakamoto, Wataru ; Huang, Jirong. / Activation of the heterotrimeric G protein α-subunit GPA1 suppresses the ftsh-mediated inhibition of chloroplast development in Arabidopsis. In: Plant Journal. 2009 ; Vol. 58, No. 6. pp. 1041-1053.
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