Activation of prothrombin by ASP, a serine protease released from Aeromonas sobria

Hidetoshi Nitta, Hidetomo Kobayashi, Atsushi Irie, Hideo Baba, Keinosuke Okamoto, Takahisa Imamura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The effect of a serine protease (ASP) secreted from Aeromonas sobria on plasma coagulation was investigated. Proteolytically active ASP promoted human plasma coagulation in a dose-dependent manner. Consistent with the preference for a factor Xa-specific oligo-peptide substrate, ASP produced enzymatic activity from human prothrombin but not from factors IX and X. ASP cleaved prothrombin to produce enzymatically active 37 kDa-fragment displaying the same molecular mass as α-thrombin. ASP is the first bacterial serine protease that produces α-thrombin, through which ASP may contribute to the induction of thrombotic tendency in disseminated intravascular coagulation complicated with sepsis caused by A. sobria infections.

Original languageEnglish
Pages (from-to)5935-5939
Number of pages5
JournalFEBS Letters
Volume581
Issue number30
DOIs
Publication statusPublished - Dec 22 2007

Fingerprint

Aeromonas
Serine Proteases
Prothrombin
Chemical activation
Coagulation
Thrombin
Plasma (human)
Factor X
Factor Xa
Factor IX
Disseminated Intravascular Coagulation
Molecular mass
Human Activities
Sepsis
Plasmas
Peptides
Substrates
Infection

Keywords

  • Aeromonas sobria
  • Bacteria
  • Coagulation
  • DIC
  • Protease
  • Prothrombin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Activation of prothrombin by ASP, a serine protease released from Aeromonas sobria. / Nitta, Hidetoshi; Kobayashi, Hidetomo; Irie, Atsushi; Baba, Hideo; Okamoto, Keinosuke; Imamura, Takahisa.

In: FEBS Letters, Vol. 581, No. 30, 22.12.2007, p. 5935-5939.

Research output: Contribution to journalArticle

Nitta, Hidetoshi ; Kobayashi, Hidetomo ; Irie, Atsushi ; Baba, Hideo ; Okamoto, Keinosuke ; Imamura, Takahisa. / Activation of prothrombin by ASP, a serine protease released from Aeromonas sobria. In: FEBS Letters. 2007 ; Vol. 581, No. 30. pp. 5935-5939.
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