Activation of protease by sol-gel entrapment into organically modified hybrid silicates

Shin Ya Furukawa, Tsutomu Ono, Hiroyuki Ijima, Koei Kawakami

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.

Original languageEnglish
Pages (from-to)13-16
Number of pages4
JournalBiotechnology Letters
Volume24
Issue number1
DOIs
Publication statusPublished - Feb 12 2002
Externally publishedYes

Keywords

  • Organic solvent
  • Organic-inorganic hybrid silicate
  • Protease
  • Sol-gel method
  • Transesterification

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

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