To prepare an immobilized protease with a high activity for transesterification of vinyl n-butyrate with 3-methylI-butanol (isoamyl alcohol) in organic media, a protease was entrapped into organic-inorganic hybrid silica gel on Celite 545 by the sol-gel method. When propyltrimethoxysilane was used as the organic silane precursor mixed with tetramethoxysilane at a molar ratio of 16:1, the hybrid gel-entrapped protease on Celite 545 had 8 times the activity of the protease deposited on Celite 545 from 35 to 85°C.
- Organic solvent
- Organic-inorganic hybrid silicate
- Sol-gel method
ASJC Scopus subject areas
- Applied Microbiology and Biotechnology