Activation of Murine Peritoneal Macrophages by Water-Soluble Extracts of Bursaphelenchus xylophilus, a Pine Wood Nematode

Hiroaki Kaji, Akihiro Tai, Kazufumi Matsushita, Hiroshi Kanzaki, Itaru Yamamoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

In our previous study, water-soluble extracts from <I>Bursaphelenchus xylophilus</I> (<I>B. xylophilus</I>), a pine wood nematode, were shown to enhance interleukin (IL)-4 plus lipopolysaccharide-induced polyclonal immunoglobulin (Ig) E production <I>in vitro</I> in mice and to increase serum levels of an antigen-nonspecific IgE <I>in vivo</I>. Here we examined whether the nematode extracts stimulate immunofunctions of murine peritoneal macrophages. In both resident and inflammatory macrophages, Fcγ receptor-mediated phagocytosis was markedly activated by <I>B. xylophilus</I> extracts, while non-specific phagocytosis was not. The enhancement of specific phagocytosis was accompanied by an increase in the formation of IgG-Fcγ receptor rosettes. <I>B. xylophilus</I> extracts also stimulated IL-1β production in both types of macrophages, and enhanced NO production and mRNA expression of inflammatory cytokines in inflammatory macrophages. These results indicate that the extracts of <I>B. xylophilus</I> contain an activating substance(s) for immunofunctions in macrophages, besides an enhancing factor for polyclonal IgE production.
Original languageEnglish
Pages (from-to)203-210
Number of pages8
JournalBioscience, Biotechnology and Biochemistry
Volume70
Issue number1
DOIs
Publication statusPublished - Jan 23 2006
Externally publishedYes

Fingerprint

Macrophages
Peritoneal Macrophages
Wood
Chemical activation
Phagocytosis
Fc Receptors
Water
Immunoglobulin E
IgG Receptors
Interleukin-1
Interleukin-4
Lipopolysaccharides
Cytokines
Antigens
Messenger RNA
Immunoglobulin G
Serum

Keywords

  • Bursaphelenchus xylophilus
  • peritoneal macrophage
  • phagocytosis
  • immunomodulator

Cite this

Activation of Murine Peritoneal Macrophages by Water-Soluble Extracts of Bursaphelenchus xylophilus, a Pine Wood Nematode. / Kaji, Hiroaki; Tai, Akihiro; Matsushita, Kazufumi; Kanzaki, Hiroshi; Yamamoto, Itaru.

In: Bioscience, Biotechnology and Biochemistry, Vol. 70, No. 1, 23.01.2006, p. 203-210.

Research output: Contribution to journalArticle

Kaji, Hiroaki ; Tai, Akihiro ; Matsushita, Kazufumi ; Kanzaki, Hiroshi ; Yamamoto, Itaru. / Activation of Murine Peritoneal Macrophages by Water-Soluble Extracts of Bursaphelenchus xylophilus, a Pine Wood Nematode. In: Bioscience, Biotechnology and Biochemistry. 2006 ; Vol. 70, No. 1. pp. 203-210.
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