Activation of lysosomal cathepsins in pregnant bovine leukocytes

Md Abdus Shabur Talukder, Ahmed Zaky Balboula, Takahiro Shirozu, Sung Woo Kim, Hiroki Kunii, Toshiyuki Suzuki, Tsukino Ito, Koji Kimura, Masashi Takahashi

Research output: Contribution to journalArticle

Abstract

In ruminants, interferon-tau (IFNT)-mediated expression of interferon-stimulated genes in peripheral blood leukocytes (PBLs) can indicate pregnancy. Recently, type 1 IFN-mediated activation of lysosomes and lysosomal cathepsins (CTSs) was observed in immune cells. This study investigated the status of lysosomal CTSs and lysosomes in PBLs collected from pregnant (P) and non-pregnant (NP) dairy cows, and conducted in vitro IFNT stimulation of NP blood leukocytes. Blood samples were collected 0, 7, 14 and 18 days post-artificial insemination, and the peripheral blood mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs) separated. The fluorescent activity of CTSB and CTSK in PMNs significantly increased with the progress of pregnancy, especially on day 18. In vitro supplementation of IFNT significantly increased the activities of CTSB and CTSK in NP PBMCs and PMNs. CTSB expression was significantly higher in PBMCs and PMNs collected from P day-18 cows than from NP cows, whereas there was no difference in CTSK expression. IFNT increased CTSB expression but did not affect CTSK expression. Immunodetection showed an increase of CTSB in P day-18 PBMCs and PMNs. In vitro stimulation of IFNT increased CTSB in NP PBMCs and PMNs. Lysosomal acidification showed a significant increase in P day-18 PBMCs and PMNs. IFNT also stimulated lysosomal acidification. Expressions of lysosome-associated membrane protein (LAMP) 1 and LAMP2 were significantly higher in P day-18 PBMCs and PMNs. The results suggest that pregnancy-specific activation of lysosomal functions by CTS activation in blood leukocytes is highly associated with IFNT during maternal and fetal recognition of pregnancy.

Original languageEnglish
Pages (from-to)515-528
Number of pages14
JournalReproduction
Volume155
Issue number6
DOIs
Publication statusPublished - Jan 1 2018

Fingerprint

Cathepsins
Granulocytes
Leukocytes
Blood Cells
Pregnancy
Lysosomes
Lysosome-Associated Membrane Glycoproteins
Artificial Insemination
Ruminants
trophoblastin
Interferons
Mothers

ASJC Scopus subject areas

  • Reproductive Medicine
  • Embryology
  • Endocrinology
  • Obstetrics and Gynaecology
  • Cell Biology

Cite this

Talukder, M. A. S., Balboula, A. Z., Shirozu, T., Kim, S. W., Kunii, H., Suzuki, T., ... Takahashi, M. (2018). Activation of lysosomal cathepsins in pregnant bovine leukocytes. Reproduction, 155(6), 515-528. https://doi.org/10.1530/REP-18-0078

Activation of lysosomal cathepsins in pregnant bovine leukocytes. / Talukder, Md Abdus Shabur; Balboula, Ahmed Zaky; Shirozu, Takahiro; Kim, Sung Woo; Kunii, Hiroki; Suzuki, Toshiyuki; Ito, Tsukino; Kimura, Koji; Takahashi, Masashi.

In: Reproduction, Vol. 155, No. 6, 01.01.2018, p. 515-528.

Research output: Contribution to journalArticle

Talukder, MAS, Balboula, AZ, Shirozu, T, Kim, SW, Kunii, H, Suzuki, T, Ito, T, Kimura, K & Takahashi, M 2018, 'Activation of lysosomal cathepsins in pregnant bovine leukocytes', Reproduction, vol. 155, no. 6, pp. 515-528. https://doi.org/10.1530/REP-18-0078
Talukder MAS, Balboula AZ, Shirozu T, Kim SW, Kunii H, Suzuki T et al. Activation of lysosomal cathepsins in pregnant bovine leukocytes. Reproduction. 2018 Jan 1;155(6):515-528. https://doi.org/10.1530/REP-18-0078
Talukder, Md Abdus Shabur ; Balboula, Ahmed Zaky ; Shirozu, Takahiro ; Kim, Sung Woo ; Kunii, Hiroki ; Suzuki, Toshiyuki ; Ito, Tsukino ; Kimura, Koji ; Takahashi, Masashi. / Activation of lysosomal cathepsins in pregnant bovine leukocytes. In: Reproduction. 2018 ; Vol. 155, No. 6. pp. 515-528.
@article{8eb56f994e894311b3d88792e9d8c4b3,
title = "Activation of lysosomal cathepsins in pregnant bovine leukocytes",
abstract = "In ruminants, interferon-tau (IFNT)-mediated expression of interferon-stimulated genes in peripheral blood leukocytes (PBLs) can indicate pregnancy. Recently, type 1 IFN-mediated activation of lysosomes and lysosomal cathepsins (CTSs) was observed in immune cells. This study investigated the status of lysosomal CTSs and lysosomes in PBLs collected from pregnant (P) and non-pregnant (NP) dairy cows, and conducted in vitro IFNT stimulation of NP blood leukocytes. Blood samples were collected 0, 7, 14 and 18 days post-artificial insemination, and the peripheral blood mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs) separated. The fluorescent activity of CTSB and CTSK in PMNs significantly increased with the progress of pregnancy, especially on day 18. In vitro supplementation of IFNT significantly increased the activities of CTSB and CTSK in NP PBMCs and PMNs. CTSB expression was significantly higher in PBMCs and PMNs collected from P day-18 cows than from NP cows, whereas there was no difference in CTSK expression. IFNT increased CTSB expression but did not affect CTSK expression. Immunodetection showed an increase of CTSB in P day-18 PBMCs and PMNs. In vitro stimulation of IFNT increased CTSB in NP PBMCs and PMNs. Lysosomal acidification showed a significant increase in P day-18 PBMCs and PMNs. IFNT also stimulated lysosomal acidification. Expressions of lysosome-associated membrane protein (LAMP) 1 and LAMP2 were significantly higher in P day-18 PBMCs and PMNs. The results suggest that pregnancy-specific activation of lysosomal functions by CTS activation in blood leukocytes is highly associated with IFNT during maternal and fetal recognition of pregnancy.",
author = "Talukder, {Md Abdus Shabur} and Balboula, {Ahmed Zaky} and Takahiro Shirozu and Kim, {Sung Woo} and Hiroki Kunii and Toshiyuki Suzuki and Tsukino Ito and Koji Kimura and Masashi Takahashi",
year = "2018",
month = "1",
day = "1",
doi = "10.1530/REP-18-0078",
language = "English",
volume = "155",
pages = "515--528",
journal = "Reproduction",
issn = "1470-1626",
publisher = "BioScientifica Ltd.",
number = "6",

}

TY - JOUR

T1 - Activation of lysosomal cathepsins in pregnant bovine leukocytes

AU - Talukder, Md Abdus Shabur

AU - Balboula, Ahmed Zaky

AU - Shirozu, Takahiro

AU - Kim, Sung Woo

AU - Kunii, Hiroki

AU - Suzuki, Toshiyuki

AU - Ito, Tsukino

AU - Kimura, Koji

AU - Takahashi, Masashi

PY - 2018/1/1

Y1 - 2018/1/1

N2 - In ruminants, interferon-tau (IFNT)-mediated expression of interferon-stimulated genes in peripheral blood leukocytes (PBLs) can indicate pregnancy. Recently, type 1 IFN-mediated activation of lysosomes and lysosomal cathepsins (CTSs) was observed in immune cells. This study investigated the status of lysosomal CTSs and lysosomes in PBLs collected from pregnant (P) and non-pregnant (NP) dairy cows, and conducted in vitro IFNT stimulation of NP blood leukocytes. Blood samples were collected 0, 7, 14 and 18 days post-artificial insemination, and the peripheral blood mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs) separated. The fluorescent activity of CTSB and CTSK in PMNs significantly increased with the progress of pregnancy, especially on day 18. In vitro supplementation of IFNT significantly increased the activities of CTSB and CTSK in NP PBMCs and PMNs. CTSB expression was significantly higher in PBMCs and PMNs collected from P day-18 cows than from NP cows, whereas there was no difference in CTSK expression. IFNT increased CTSB expression but did not affect CTSK expression. Immunodetection showed an increase of CTSB in P day-18 PBMCs and PMNs. In vitro stimulation of IFNT increased CTSB in NP PBMCs and PMNs. Lysosomal acidification showed a significant increase in P day-18 PBMCs and PMNs. IFNT also stimulated lysosomal acidification. Expressions of lysosome-associated membrane protein (LAMP) 1 and LAMP2 were significantly higher in P day-18 PBMCs and PMNs. The results suggest that pregnancy-specific activation of lysosomal functions by CTS activation in blood leukocytes is highly associated with IFNT during maternal and fetal recognition of pregnancy.

AB - In ruminants, interferon-tau (IFNT)-mediated expression of interferon-stimulated genes in peripheral blood leukocytes (PBLs) can indicate pregnancy. Recently, type 1 IFN-mediated activation of lysosomes and lysosomal cathepsins (CTSs) was observed in immune cells. This study investigated the status of lysosomal CTSs and lysosomes in PBLs collected from pregnant (P) and non-pregnant (NP) dairy cows, and conducted in vitro IFNT stimulation of NP blood leukocytes. Blood samples were collected 0, 7, 14 and 18 days post-artificial insemination, and the peripheral blood mononuclear cells (PBMCs) and polymorphonuclear granulocytes (PMNs) separated. The fluorescent activity of CTSB and CTSK in PMNs significantly increased with the progress of pregnancy, especially on day 18. In vitro supplementation of IFNT significantly increased the activities of CTSB and CTSK in NP PBMCs and PMNs. CTSB expression was significantly higher in PBMCs and PMNs collected from P day-18 cows than from NP cows, whereas there was no difference in CTSK expression. IFNT increased CTSB expression but did not affect CTSK expression. Immunodetection showed an increase of CTSB in P day-18 PBMCs and PMNs. In vitro stimulation of IFNT increased CTSB in NP PBMCs and PMNs. Lysosomal acidification showed a significant increase in P day-18 PBMCs and PMNs. IFNT also stimulated lysosomal acidification. Expressions of lysosome-associated membrane protein (LAMP) 1 and LAMP2 were significantly higher in P day-18 PBMCs and PMNs. The results suggest that pregnancy-specific activation of lysosomal functions by CTS activation in blood leukocytes is highly associated with IFNT during maternal and fetal recognition of pregnancy.

UR - http://www.scopus.com/inward/record.url?scp=85047316533&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85047316533&partnerID=8YFLogxK

U2 - 10.1530/REP-18-0078

DO - 10.1530/REP-18-0078

M3 - Article

VL - 155

SP - 515

EP - 528

JO - Reproduction

JF - Reproduction

SN - 1470-1626

IS - 6

ER -