Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis

Wataru Matsuyama, Masaki Watanabe, Yuko Shirahama, Ken Ichi Oonakahara, Ikkou Higashimoto, Teizo Yoshimura, Mitsuhiro Osame, Kimiyoshi Arimura

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Discoidin domain receptor 1 (DDR1) is a receptor tyrosine kinase activated by collagen. We previously reported the functional expression of DDR1 on human monocyte-derived macrophages in vitro; however, information regarding its role in diseases is limited. Idiopathic pulmonary fibrosis (IPF) is a chronic lung disease, and the lesions contain an abundance of collagen. In this study, we examined DDR1 expression on bronchoalveolar lavage fluid (BALF) cells and investigated its functionality using samples obtained from 28 IPF patients, 13 chronic obstructive pulmonary disease patients, and 14 healthy volunteers. The DDR1 expression level in CD14-positive BALF cells was higher in IPF patients than in chronic obstructive pulmonary disease patients or healthy volunteers. The predominant isoform was DDR1b in the IPF group, while DDR1a was predominant in the other two groups. Using immunohistochemical analysis, we also detected DDR1 expression on infiltrating inflammatory cells in the IPF lesion. In IPF patients, DDR1 activation induced the production of MCP-1, IL-8, MIP-1 α, and matrix metalloproteinase-9 (MMP-9) from CD14-positive BALF cells in a p38 MAPK-dependent manner. In contrast, DDRl activation of CD14-positive BALF cells in the other groups did not induce the production of these chemokines or MMP-9. These chemokines and MMP-9 contribute to the development of IPF and, therefore, we suggest that DDR1 might be associated with the pathogenesis of IPF in the tissue microenvironment.

Original languageEnglish
Pages (from-to)6490-6498
Number of pages9
JournalJournal of Immunology
Volume174
Issue number10
Publication statusPublished - May 15 2005
Externally publishedYes

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Idiopathic Pulmonary Fibrosis
Bronchoalveolar Lavage Fluid
Chemokines
Matrix Metalloproteinase 9
Chronic Obstructive Pulmonary Disease
Healthy Volunteers
Collagen
Discoidin Domain Receptor 1
Receptor Protein-Tyrosine Kinases
p38 Mitogen-Activated Protein Kinases
Interleukin-8
Lung Diseases
Protein Isoforms
Chronic Disease
Macrophages

ASJC Scopus subject areas

  • Immunology

Cite this

Matsuyama, W., Watanabe, M., Shirahama, Y., Oonakahara, K. I., Higashimoto, I., Yoshimura, T., ... Arimura, K. (2005). Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis. Journal of Immunology, 174(10), 6490-6498.

Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis. / Matsuyama, Wataru; Watanabe, Masaki; Shirahama, Yuko; Oonakahara, Ken Ichi; Higashimoto, Ikkou; Yoshimura, Teizo; Osame, Mitsuhiro; Arimura, Kimiyoshi.

In: Journal of Immunology, Vol. 174, No. 10, 15.05.2005, p. 6490-6498.

Research output: Contribution to journalArticle

Matsuyama, W, Watanabe, M, Shirahama, Y, Oonakahara, KI, Higashimoto, I, Yoshimura, T, Osame, M & Arimura, K 2005, 'Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis', Journal of Immunology, vol. 174, no. 10, pp. 6490-6498.
Matsuyama, Wataru ; Watanabe, Masaki ; Shirahama, Yuko ; Oonakahara, Ken Ichi ; Higashimoto, Ikkou ; Yoshimura, Teizo ; Osame, Mitsuhiro ; Arimura, Kimiyoshi. / Activation of discoidin domain receptor 1 on CD14-positive bronchoalveolar lavage fluid cells induces chemokine production in idiopathic pulmonary fibrosis. In: Journal of Immunology. 2005 ; Vol. 174, No. 10. pp. 6490-6498.
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