Activation of cell-survival signal Akt by GDNF in normal rat brain

G. Jin, N. Omori, F. Li, K. Sato, I. Nagano, Y. Manabe, M. Shoji, Koji Abe

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.

Original languageEnglish
Pages (from-to)429-433
Number of pages5
JournalBrain Research
Volume958
Issue number2
DOIs
Publication statusPublished - Dec 27 2002

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Glial Cell Line-Derived Neurotrophic Factor
Cell Survival
Brain
Phosphorylation
Cerebrum
Protein Kinases

Keywords

  • Glial cell line-derived neurotrophic factor
  • Phospho-Akt
  • Rat

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Activation of cell-survival signal Akt by GDNF in normal rat brain. / Jin, G.; Omori, N.; Li, F.; Sato, K.; Nagano, I.; Manabe, Y.; Shoji, M.; Abe, Koji.

In: Brain Research, Vol. 958, No. 2, 27.12.2002, p. 429-433.

Research output: Contribution to journalArticle

Jin, G, Omori, N, Li, F, Sato, K, Nagano, I, Manabe, Y, Shoji, M & Abe, K 2002, 'Activation of cell-survival signal Akt by GDNF in normal rat brain', Brain Research, vol. 958, no. 2, pp. 429-433. https://doi.org/10.1016/S0006-8993(02)03521-7
Jin G, Omori N, Li F, Sato K, Nagano I, Manabe Y et al. Activation of cell-survival signal Akt by GDNF in normal rat brain. Brain Research. 2002 Dec 27;958(2):429-433. https://doi.org/10.1016/S0006-8993(02)03521-7
Jin, G. ; Omori, N. ; Li, F. ; Sato, K. ; Nagano, I. ; Manabe, Y. ; Shoji, M. ; Abe, Koji. / Activation of cell-survival signal Akt by GDNF in normal rat brain. In: Brain Research. 2002 ; Vol. 958, No. 2. pp. 429-433.
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