Activation of cell-survival signal Akt by GDNF in normal rat brain

G. Jin; N. Omori; F. Li; K. Sato; I. Nagano; Y. Manabe; M. Shoji; K. Abe

doi:10.1016/S0006-8993(02)03521-7

Activation of cell-survival signal Akt by GDNF in normal rat brain

G. Jin, N. Omori, F. Li, K. Sato, I. Nagano, Y. Manabe, M. Shoji, K. Abe

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.

Original language	English
Pages (from-to)	429-433
Number of pages	5
Journal	Brain Research
Volume	958
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(02)03521-7
Publication status	Published - Dec 27 2002

Keywords

Glial cell line-derived neurotrophic factor
Phospho-Akt
Rat

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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title = "Activation of cell-survival signal Akt by GDNF in normal rat brain",

abstract = "Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.",

keywords = "Glial cell line-derived neurotrophic factor, Phospho-Akt, Rat",

author = "G. Jin and N. Omori and F. Li and K. Sato and I. Nagano and Y. Manabe and M. Shoji and K. Abe",

note = "Funding Information: This work was partly supported by Grants-in-Aid for Scientific Research (B) 12470141, (Hoga) 12877211, and (Shorei) 13770377 from the Ministry of Education, Science, Culture and Sports of Japan, and by Grants (K. Tashiro, Y. Itoyama) and Comprehensive Research on Aging and Health (H11-Choju-010, No. 207, A. Koizumi) from the Ministry of Health and Welfare of Japan.",

year = "2002",

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doi = "10.1016/S0006-8993(02)03521-7",

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T1 - Activation of cell-survival signal Akt by GDNF in normal rat brain

AU - Jin, G.

AU - Omori, N.

AU - Li, F.

AU - Sato, K.

AU - Nagano, I.

AU - Manabe, Y.

AU - Shoji, M.

AU - Abe, K.

N1 - Funding Information: This work was partly supported by Grants-in-Aid for Scientific Research (B) 12470141, (Hoga) 12877211, and (Shorei) 13770377 from the Ministry of Education, Science, Culture and Sports of Japan, and by Grants (K. Tashiro, Y. Itoyama) and Comprehensive Research on Aging and Health (H11-Choju-010, No. 207, A. Koizumi) from the Ministry of Health and Welfare of Japan.

PY - 2002/12/27

Y1 - 2002/12/27

N2 - Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.

AB - Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.

KW - Glial cell line-derived neurotrophic factor

KW - Phospho-Akt

KW - Rat

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