TY - JOUR
T1 - Activation of cell-survival signal Akt by GDNF in normal rat brain
AU - Jin, G.
AU - Omori, N.
AU - Li, F.
AU - Sato, K.
AU - Nagano, I.
AU - Manabe, Y.
AU - Shoji, M.
AU - Abe, K.
N1 - Funding Information:
This work was partly supported by Grants-in-Aid for Scientific Research (B) 12470141, (Hoga) 12877211, and (Shorei) 13770377 from the Ministry of Education, Science, Culture and Sports of Japan, and by Grants (K. Tashiro, Y. Itoyama) and Comprehensive Research on Aging and Health (H11-Choju-010, No. 207, A. Koizumi) from the Ministry of Health and Welfare of Japan.
PY - 2002/12/27
Y1 - 2002/12/27
N2 - Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.
AB - Glial cell line-derived neurotrophic factor (GDNF) activates protein kinase Akt/PKB by phosphorylation (p-Akt) which plays key roles in cell survival. In the current study, we investigated a temporal expression of p-Akt by immunohistochemical analysis after a topical application of GDNF to normal cerebral hemisphere of rats. Although p-Akt immunoreactivity was weakly present in the sham control neural cells, GDNF application greatly enhanced it at 3 h, which lasted until 1 day. These results indicate that p-Akt is expressed in neuronal cells under physiological conditions, and that topical application of GDNF greatly enhanced the phosphorylation of Akt in normal rat brain.
KW - Glial cell line-derived neurotrophic factor
KW - Phospho-Akt
KW - Rat
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U2 - 10.1016/S0006-8993(02)03521-7
DO - 10.1016/S0006-8993(02)03521-7
M3 - Article
C2 - 12470880
AN - SCOPUS:0037184694
VL - 958
SP - 429
EP - 433
JO - Molecular Brain Research
JF - Molecular Brain Research
SN - 0006-8993
IS - 2
ER -