Activation mechanism of human Hageman factor-plasma kallikreinkinin system by Vibrio vulnificus metalloprotease

Shin ichi Miyoshi, Sumio Shinoda

Research output: Contribution to journalArticlepeer-review

38 Citations (Scopus)

Abstract

Vibro vulnificus, an opportunistic human pathogen, secretes a metalloprotease (VVP). The VVP inoculated into a guinea pig is known to generate bradykinin through activation of the Hageman factor-plasma kallikrein-kinin system. VVP was shown to poisess the ability to activate the human system through the same mechanism as that clarified in the guinea pig system, namely, VVP converted both human zymogens (Hageman factor and plasma prekallikrein) to active enzymes (activated Hageman factor and plasma kallikrein), and the then generated kallikrein liberated bradykinin from high molecular-weight kininogen. However, in the presence of plasma α2-macroglobulin (α2M), the VVP action was drastically decreased. This finding suggests that the human system might be activated only at the interstitial-tissue space which contains negligible amounts of α2M or in the bloodstream of the individuals whom plasma α2M level is extremely reduced.

Original languageEnglish
Pages (from-to)315-319
Number of pages5
JournalFEBS Letters
Volume308
Issue number3
DOIs
Publication statusPublished - Aug 24 1992

Keywords

  • Bacterial metalloprotease
  • Kinin generation
  • Vibro

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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