TY - JOUR
T1 - Activation mechanism of human Hageman factor-plasma kallikreinkinin system by Vibrio vulnificus metalloprotease
AU - Miyoshi, Shin ichi
AU - Shinoda, Sumio
N1 - Funding Information:
/Ick~Jowlrdgrmemx: This work was supported by a gnmt.in.aid for scientific research from the Ministry of Education. Science, and Cal. tare of Jap;m.
PY - 1992/8/24
Y1 - 1992/8/24
N2 - Vibro vulnificus, an opportunistic human pathogen, secretes a metalloprotease (VVP). The VVP inoculated into a guinea pig is known to generate bradykinin through activation of the Hageman factor-plasma kallikrein-kinin system. VVP was shown to poisess the ability to activate the human system through the same mechanism as that clarified in the guinea pig system, namely, VVP converted both human zymogens (Hageman factor and plasma prekallikrein) to active enzymes (activated Hageman factor and plasma kallikrein), and the then generated kallikrein liberated bradykinin from high molecular-weight kininogen. However, in the presence of plasma α2-macroglobulin (α2M), the VVP action was drastically decreased. This finding suggests that the human system might be activated only at the interstitial-tissue space which contains negligible amounts of α2M or in the bloodstream of the individuals whom plasma α2M level is extremely reduced.
AB - Vibro vulnificus, an opportunistic human pathogen, secretes a metalloprotease (VVP). The VVP inoculated into a guinea pig is known to generate bradykinin through activation of the Hageman factor-plasma kallikrein-kinin system. VVP was shown to poisess the ability to activate the human system through the same mechanism as that clarified in the guinea pig system, namely, VVP converted both human zymogens (Hageman factor and plasma prekallikrein) to active enzymes (activated Hageman factor and plasma kallikrein), and the then generated kallikrein liberated bradykinin from high molecular-weight kininogen. However, in the presence of plasma α2-macroglobulin (α2M), the VVP action was drastically decreased. This finding suggests that the human system might be activated only at the interstitial-tissue space which contains negligible amounts of α2M or in the bloodstream of the individuals whom plasma α2M level is extremely reduced.
KW - Bacterial metalloprotease
KW - Kinin generation
KW - Vibro
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U2 - 10.1016/0014-5793(92)81301-2
DO - 10.1016/0014-5793(92)81301-2
M3 - Article
C2 - 1380471
AN - SCOPUS:0026764605
VL - 308
SP - 315
EP - 319
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 3
ER -