Activation and epigenetic regulation of DNA transposon nDart1 in rice

Chang Ho Eun, Kyoko Takagi, Kyeung Il Park, Masahiko Maekawa, Shigeru Iida, Kazuo Tsugane

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

A large part of the rice genome is composed of transposons. Since active excision/reintegration of these mobile elements may result in harmful genetic changes, many transposons are maintained in a genetically or epigenetically inactivated state. However, some non-autonomous DNA transposons of the nDart1-3 subgroup, including nDart1-0, actively transpose in specific rice lines, such as pyl-v which carries an active autonomous element, aDart1-27, on chromosome 6. Although nDart1-3 subgroup elements show considerable sequence identity, they display different excision frequencies. The most active element, nDart1-0, had a low cytosine methylation status. The aDart1-27 sequence showed conservation between pyl-stb (pyl-v derivative line) and Nipponbare, which both lack autonomous activity for transposition of nDart1-3 subgroup elements. In pyl-v plants, the promoter region of the aDart1-27 transposase gene was more hypomethylated than in other rice lines. Treatment with the methylation inhibitor 5-azacytidine (5-azaC) induced transposition of nDart1-3 subgroup elements in both pyl-stb and Nipponbare plants; the new insertion sites were frequently located in genic regions. 5-AzaC treatment principally induced expression of Dart1-34 transposase rather than the other 38 aDart1-related elements in both pyl-stb and Nipponbare treatment groups. Our observations show that transposition of nDart1-3 subgroup elements in the nDart1/aDart1 tagging system is correlated with the level of DNA methylation. Our system does not cause somaclonal variation due to an absence of transformed plants, offers the possibility of large-scale screening in the field and can identify dominant mutants. We therefore propose that this tagging system provides a valuable addition to the tools available for rice functional genomics.

Original languageEnglish
Pages (from-to)857-868
Number of pages12
JournalPlant and Cell Physiology
Volume53
Issue number5
DOIs
Publication statusPublished - May 2012

Fingerprint

DNA Transposable Elements
Epigenomics
epigenetics
transposons
transposition (genetics)
Transposases
rice
DNA
methylation
Methylation
Azacitidine
Chromosomes, Human, Pair 6
somaclonal variation
cytosine
Cytosine
DNA methylation
DNA Methylation
Genomics
Genetic Promoter Regions
chemical derivatives

Keywords

  • 5-AzaC
  • DNA transposons
  • Functional genomics
  • Induced transpositions
  • Rice

ASJC Scopus subject areas

  • Plant Science
  • Physiology
  • Cell Biology

Cite this

Activation and epigenetic regulation of DNA transposon nDart1 in rice. / Eun, Chang Ho; Takagi, Kyoko; Park, Kyeung Il; Maekawa, Masahiko; Iida, Shigeru; Tsugane, Kazuo.

In: Plant and Cell Physiology, Vol. 53, No. 5, 05.2012, p. 857-868.

Research output: Contribution to journalArticle

Eun, Chang Ho ; Takagi, Kyoko ; Park, Kyeung Il ; Maekawa, Masahiko ; Iida, Shigeru ; Tsugane, Kazuo. / Activation and epigenetic regulation of DNA transposon nDart1 in rice. In: Plant and Cell Physiology. 2012 ; Vol. 53, No. 5. pp. 857-868.
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