Abstract
We investigated the immune responses of patients with cholestatic and hepatitis C virus-positive (HCV-positive) liver cirrhosis by analysing T-cell subsets and cytokine levels in the portal and peripheral veins, using flow cytometry and enzyme-linked immunosorbent assay. In cholestatic liver cirrhosis, the proportion of natural-killer (NK) T cells and interleukin (IL) 6 and IL-18 levels in the portal venous blood were significantly higher than those in the peripheral venous blood. In HCV-positive liver cirrhosis, the proportions of NK T cells and Fas+ T cells and IL-6 and soluble Fas levels in the portal venous blood were significantly higher than those in the peripheral venous blood. These results suggest that in these diseases, activated T cells and soluble molecules in portal venous blood may promote Fas/FasL-mediated apoptosis of the bile-duct cells and hepatocytes, and contribute to the deterioration in liver function as an inevitable result of positive feedback.
| Original language | English |
|---|---|
| Pages (from-to) | 170-180 |
| Number of pages | 11 |
| Journal | Journal of International Medical Research |
| Volume | 31 |
| Issue number | 3 |
| Publication status | Published - May 2003 |
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Keywords
- Apoptosis
- Cholestatic liver cirrhosis
- Fas
- Fas-ligand
- HCV-positive liver cirrhosis
- Interleukin 18
- Interleukin 6
- Natural-killer T cells
ASJC Scopus subject areas
- Medicine(all)
Cite this
Activated T cells and soluble molecules in the portal venous blood of patients with cholestatic and hepatitis C virus-positive liver cirrhosis. Possible promotion of Fas/FasL-mediated apoptosis in the bile-duct cells and hepatocyte injury. / Ariki, N.; Morimoto, Y.; Yagi, Takahito; Oyama, T.; Cyouda, Y.; Sadamori, H.; Inagaki, M.; Urushihara, N.; Iwagaki, H.; Tanaka, N.
In: Journal of International Medical Research, Vol. 31, No. 3, 05.2003, p. 170-180.Research output: Contribution to journal › Article
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TY - JOUR
T1 - Activated T cells and soluble molecules in the portal venous blood of patients with cholestatic and hepatitis C virus-positive liver cirrhosis. Possible promotion of Fas/FasL-mediated apoptosis in the bile-duct cells and hepatocyte injury
AU - Ariki, N.
AU - Morimoto, Y.
AU - Yagi, Takahito
AU - Oyama, T.
AU - Cyouda, Y.
AU - Sadamori, H.
AU - Inagaki, M.
AU - Urushihara, N.
AU - Iwagaki, H.
AU - Tanaka, N.
PY - 2003/5
Y1 - 2003/5
N2 - We investigated the immune responses of patients with cholestatic and hepatitis C virus-positive (HCV-positive) liver cirrhosis by analysing T-cell subsets and cytokine levels in the portal and peripheral veins, using flow cytometry and enzyme-linked immunosorbent assay. In cholestatic liver cirrhosis, the proportion of natural-killer (NK) T cells and interleukin (IL) 6 and IL-18 levels in the portal venous blood were significantly higher than those in the peripheral venous blood. In HCV-positive liver cirrhosis, the proportions of NK T cells and Fas+ T cells and IL-6 and soluble Fas levels in the portal venous blood were significantly higher than those in the peripheral venous blood. These results suggest that in these diseases, activated T cells and soluble molecules in portal venous blood may promote Fas/FasL-mediated apoptosis of the bile-duct cells and hepatocytes, and contribute to the deterioration in liver function as an inevitable result of positive feedback.
AB - We investigated the immune responses of patients with cholestatic and hepatitis C virus-positive (HCV-positive) liver cirrhosis by analysing T-cell subsets and cytokine levels in the portal and peripheral veins, using flow cytometry and enzyme-linked immunosorbent assay. In cholestatic liver cirrhosis, the proportion of natural-killer (NK) T cells and interleukin (IL) 6 and IL-18 levels in the portal venous blood were significantly higher than those in the peripheral venous blood. In HCV-positive liver cirrhosis, the proportions of NK T cells and Fas+ T cells and IL-6 and soluble Fas levels in the portal venous blood were significantly higher than those in the peripheral venous blood. These results suggest that in these diseases, activated T cells and soluble molecules in portal venous blood may promote Fas/FasL-mediated apoptosis of the bile-duct cells and hepatocytes, and contribute to the deterioration in liver function as an inevitable result of positive feedback.
KW - Apoptosis
KW - Cholestatic liver cirrhosis
KW - Fas
KW - Fas-ligand
KW - HCV-positive liver cirrhosis
KW - Interleukin 18
KW - Interleukin 6
KW - Natural-killer T cells
UR - http://www.scopus.com/inward/record.url?scp=10744226853&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=10744226853&partnerID=8YFLogxK
M3 - Article
VL - 31
SP - 170
EP - 180
JO - Journal of International Medical Research
JF - Journal of International Medical Research
SN - 0300-0605
IS - 3
ER -