Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells

Cun Lin, Yawei Yu, Takashi Kadono, Michiaki Iwata, Kenji Umemura, Takuya Furuichi, Masaki Kuse, Minoru Isobe, Yoko Yamamoto, Hideaki Matsumoto, Kazuharu Yoshizuka, Tomonori Kawano

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31 Citations (Scopus)

Abstract

Previously, effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana, AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism.

Original languageEnglish
Pages (from-to)823-830
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume332
Issue number3
DOIs
Publication statusPublished - Jul 8 2005

Keywords

  • Aequorin
  • Al
  • Ca
  • Cold shock
  • Nicotiana tabacum
  • Salicylic acid

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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  • Cite this

    Lin, C., Yu, Y., Kadono, T., Iwata, M., Umemura, K., Furuichi, T., Kuse, M., Isobe, M., Yamamoto, Y., Matsumoto, H., Yoshizuka, K., & Kawano, T. (2005). Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells. Biochemical and Biophysical Research Communications, 332(3), 823-830. https://doi.org/10.1016/j.bbrc.2005.05.030