Abstract
Previously, effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana, AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism.
| Original language | English |
|---|---|
| Pages (from-to) | 823-830 |
| Number of pages | 8 |
| Journal | Biochemical and Biophysical Research Communications |
| Volume | 332 |
| Issue number | 3 |
| DOIs | |
| Publication status | Published - Jul 8 2005 |
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Keywords
- Aequorin
- Al
- Ca
- Cold shock
- Nicotiana tabacum
- Salicylic acid
ASJC Scopus subject areas
- Biochemistry
- Biophysics
- Molecular Biology
Cite this
Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells. / Lin, Cun; Yu, Yawei; Kadono, Takashi; Iwata, Michiaki; Umemura, Kenji; Furuichi, Takuya; Kuse, Masaki; Isobe, Minoru; Yamamoto, Yoko; Matsumoto, Hideaki; Yoshizuka, Kazuharu; Kawano, Tomonori.
In: Biochemical and Biophysical Research Communications, Vol. 332, No. 3, 08.07.2005, p. 823-830.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells
AU - Lin, Cun
AU - Yu, Yawei
AU - Kadono, Takashi
AU - Iwata, Michiaki
AU - Umemura, Kenji
AU - Furuichi, Takuya
AU - Kuse, Masaki
AU - Isobe, Minoru
AU - Yamamoto, Yoko
AU - Matsumoto, Hideaki
AU - Yoshizuka, Kazuharu
AU - Kawano, Tomonori
PY - 2005/7/8
Y1 - 2005/7/8
N2 - Previously, effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana, AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism.
AB - Previously, effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana, AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism.
KW - Aequorin
KW - Al
KW - Ca
KW - Cold shock
KW - Nicotiana tabacum
KW - Salicylic acid
UR - http://www.scopus.com/inward/record.url?scp=19744377046&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=19744377046&partnerID=8YFLogxK
U2 - 10.1016/j.bbrc.2005.05.030
DO - 10.1016/j.bbrc.2005.05.030
M3 - Article
C2 - 15913561
AN - SCOPUS:19744377046
VL - 332
SP - 823
EP - 830
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
SN - 0006-291X
IS - 3
ER -