Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells

Cun Lin, Yawei Yu, Takashi Kadono, Michiaki Iwata, Kenji Umemura, Takuya Furuichi, Masaki Kuse, Minoru Isobe, Yoko Yamamoto, Hideaki Matsumoto, Kazuharu Yoshizuka, Tomonori Kawano

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Previously, effect of Al ions on calcium signaling was assessed in tobacco cells expressing a Ca2+-monitoring luminescent protein, aequorin and a newly isolated putative plant Ca2+ channel protein from Arabidopsis thaliana, AtTPC1 (two-pore channel 1). TPC1 channels were shown to be the only channel known to be sensitive to Al and they are responsive to reactive oxygen species and cryptogein, a fungal elicitor protein. Thus, involvement of TPC1 channels in calcium signaling leading to development of plant defense mechanism has been suggested. Then, the use of Al as a specific inhibitor of TPC1-type plant calcium channels has been proposed. Here, using transgenic tobacco BY-2 cells expressing aequorin, we report on the evidence in support of the involvement of Al-sensitive signaling pathway requiring TPC1-type channel-dependent Ca2+ influx in response to salicylic acid, a key plant defense-inducing agent, but not to an elicitor prepared from the cell wall of rice blast disease fungus Magnaporthe grisea. In addition, involvement of Al-sensitive Ca2+ channels in response to cold shock was also tested. The data suggested that the elicitor used here induces the Ca2+ influx via Al-insensitive path, while salicylic acid and cold-shock-stimulate the influx of Ca2+ via Al-sensitive mechanism.

Original languageEnglish
Pages (from-to)823-830
Number of pages8
JournalBiochemical and Biophysical Research Communications
Volume332
Issue number3
DOIs
Publication statusPublished - Jul 8 2005

Fingerprint

Tobacco
Salicylates
Aluminum
Aequorin
Shock
Calcium Signaling
Salicylic Acid
Cells
Calcium
Cold-Shock Response
Luminescent Proteins
Magnaporthe
Arabidopsis Proteins
Fungal Proteins
Plant Development
Mycoses
Calcium Channels
Fungi
Cell Wall
Reactive Oxygen Species

Keywords

  • Aequorin
  • Al
  • Ca
  • Cold shock
  • Nicotiana tabacum
  • Salicylic acid

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells. / Lin, Cun; Yu, Yawei; Kadono, Takashi; Iwata, Michiaki; Umemura, Kenji; Furuichi, Takuya; Kuse, Masaki; Isobe, Minoru; Yamamoto, Yoko; Matsumoto, Hideaki; Yoshizuka, Kazuharu; Kawano, Tomonori.

In: Biochemical and Biophysical Research Communications, Vol. 332, No. 3, 08.07.2005, p. 823-830.

Research output: Contribution to journalArticle

Lin, C, Yu, Y, Kadono, T, Iwata, M, Umemura, K, Furuichi, T, Kuse, M, Isobe, M, Yamamoto, Y, Matsumoto, H, Yoshizuka, K & Kawano, T 2005, 'Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells', Biochemical and Biophysical Research Communications, vol. 332, no. 3, pp. 823-830. https://doi.org/10.1016/j.bbrc.2005.05.030
Lin, Cun ; Yu, Yawei ; Kadono, Takashi ; Iwata, Michiaki ; Umemura, Kenji ; Furuichi, Takuya ; Kuse, Masaki ; Isobe, Minoru ; Yamamoto, Yoko ; Matsumoto, Hideaki ; Yoshizuka, Kazuharu ; Kawano, Tomonori. / Action of aluminum, novel TPC1-type channel inhibitor, against salicylate-induced and cold-shock-induced calcium influx in tobacco BY-2 cells. In: Biochemical and Biophysical Research Communications. 2005 ; Vol. 332, No. 3. pp. 823-830.
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