Acridine orange as a fluorescent probe for lysosomal proton pump

Yoshinori Moriyama, Tatsuya Takano, Shoji Ohkuma

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

Acridine orange was found to accumulate in pure lysosomal particles (tritosomes) in vitro, and the quenching of its fluorescence correlated well with the ΔpH (inside acid) across the lysosomal membrane. Use of this dye showed that Mg-ATP caused lysosomal acidification. This acidification was sensitive to N,N'-dicyclohexylcar-bodiimide, N-ethylmaleimide, and azide, but not to oligomycin, ouabain or vanadate. These results supported the idea of the existence of a lysosomal H+-pump, suggested in a previous paper (Ohkuma et al. (1982) Proc. Natl. Acad. Sci. U.S. 79, 2758-2762).

Original languageEnglish
Pages (from-to)1333-1336
Number of pages4
JournalJournal of biochemistry
Volume92
Issue number4
DOIs
Publication statusPublished - Oct 1982

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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