Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

Bunichi Ezaki, Mayandi Sivaguru, Yuka Ezaki, Hideaki Matsumoto, Richard C. Gardner

    Research output: Contribution to journalArticlepeer-review

    37 Citations (Scopus)

    Abstract

    Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.

    Original languageEnglish
    Pages (from-to)81-87
    Number of pages7
    JournalFEMS Microbiology Letters
    Volume171
    Issue number2
    DOIs
    Publication statusPublished - Feb 15 1999

    Keywords

    • Aluminum metal stress
    • BCB gene
    • Morin
    • NtGDI1 gene
    • Tolerant mechanism
    • Vesicle transport

    ASJC Scopus subject areas

    • Microbiology
    • Molecular Biology
    • Genetics

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