Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants

Bunichi Ezaki, Mayandi Sivaguru, Yuka Ezaki, Hideaki Matsumoto, Richard C. Gardner

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Eleven aluminum stress-induced genes derived from plants (wheat, Arabidopsis and tobacco) were introduced into Saccharomyces cerevisiae to test if expression of these genes confers A1 tolerance. A1 sensitivity tests showed that expression of two genes, either an Arabidopsis gene for blue copper binding protein (BCB), or a tobacco gene for the GDP dissociation inhibitor (NtGDI1), conferred A1 tolerance. Determinations of total content and localization of A1 ions in these transformants suggested that the BCB gene product functions in restricting A1 uptake, while expression of the NtGDI1 gene promotes release of A1 ions after uptake.

Original languageEnglish
Pages (from-to)81-87
Number of pages7
JournalFEMS Microbiology Letters
Volume171
Issue number2
DOIs
Publication statusPublished - Feb 15 1999

Fingerprint

Plant Genes
Aluminum
Saccharomyces cerevisiae
Gene Expression
Tobacco
Guanine Nucleotide Dissociation Inhibitors
Ions
Arabidopsis
Genes
Triticum
copper-binding protein
Proteins

Keywords

  • Aluminum metal stress
  • BCB gene
  • Morin
  • NtGDI1 gene
  • Tolerant mechanism
  • Vesicle transport

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Applied Microbiology and Biotechnology
  • Microbiology

Cite this

Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants. / Ezaki, Bunichi; Sivaguru, Mayandi; Ezaki, Yuka; Matsumoto, Hideaki; Gardner, Richard C.

In: FEMS Microbiology Letters, Vol. 171, No. 2, 15.02.1999, p. 81-87.

Research output: Contribution to journalArticle

Ezaki, Bunichi ; Sivaguru, Mayandi ; Ezaki, Yuka ; Matsumoto, Hideaki ; Gardner, Richard C. / Acquisition of aluminum tolerance in Saccharomyces cerevisiae by expression of the BCB or NtGDI1 gene derived from plants. In: FEMS Microbiology Letters. 1999 ; Vol. 171, No. 2. pp. 81-87.
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