TY - JOUR
T1 - Acidic polysaccharides isolated from marine algae inhibit the early step of viral infection
AU - Ueno, Mikinori
AU - Nogawa, Masato
AU - Siddiqui, Rokeya
AU - Watashi, Koichi
AU - Wakita, Takaji
AU - Kato, Nobuyuki
AU - Ikeda, Masanori
AU - Okimura, Takasi
AU - Isaka, Shogo
AU - Oda, Tatsuya
AU - Ariumi, Yasuo
N1 - Funding Information:
This work was supported by the Research Program on Hepatitis from the Japan Agency for Medical Research and Development (AMED) and Japan Society for the Promotion of Science (JSPS), and an AIDS International Collaborative Research Grant from the Ministry of Education, Culture, Sports, Science and Technology (MEXT), and Kumamoto University . This work was also supported by a Grant-in-Aid for JSPS Research Fellow ( 18J00112 ). We would like to thank Drs. Didier Trono (Ecole Polytechnique Fédérale de Lausanne) and Yoshio Koyanagi (Kyoto University) for HIV-1 molecular clones and plasmids. We are grateful for Mr. Hiroshi Nomura (Immuno Probe Co., Ltd.) for anti-HIV-1 p24 mouse monoclonal antibody. We also thank Dr. Osamu Noyori (Kumamoto University) for technical assistance.
Publisher Copyright:
© 2018 Elsevier B.V.
PY - 2019/3/1
Y1 - 2019/3/1
N2 - We examined the effects of various acidic polysaccharides isolated from marine algae on the infection and replication of human immunodeficiency virus type-1 (HIV-1), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-cell leukemia virus type-1 (HTLV-1). It was found that sulfated fucan polysaccharides, ascophyllan, and two fucoidans derived from different sources significantly inhibited the early step of HIV-1 (R9 and JR-FL) infection, while they did not affect the late step. The alginate oligomer consisted of uronic acids and sulfated-galactan porphyran showed no significant inhibitory effects. In addition, ascophyllan and two fucoidans inhibited the early step of HBV infection in a dose-dependent manner. Furthermore, these polysaccharides inhibited the early step of HCV infection but had no inhibitory effects on HTLV-1 replication. To further examine the specificity of these polysaccharides in viral infections, we used vesicular stomatitis virus (VSV)-G-pseudotyped HIV-1 infection. Ascophyllan, the two fucoidans, and alginate oligomer also potently inhibited VSV-G-pseudotyped HIV-1 infection in HeLa cells. Taken together, these results suggest that the acidic polysaccharides used in this study are capable of inhibiting the early step of viral infections depending on the polysaccharides but not in a strict species-specific manner.
AB - We examined the effects of various acidic polysaccharides isolated from marine algae on the infection and replication of human immunodeficiency virus type-1 (HIV-1), hepatitis B virus (HBV), hepatitis C virus (HCV), and human T-cell leukemia virus type-1 (HTLV-1). It was found that sulfated fucan polysaccharides, ascophyllan, and two fucoidans derived from different sources significantly inhibited the early step of HIV-1 (R9 and JR-FL) infection, while they did not affect the late step. The alginate oligomer consisted of uronic acids and sulfated-galactan porphyran showed no significant inhibitory effects. In addition, ascophyllan and two fucoidans inhibited the early step of HBV infection in a dose-dependent manner. Furthermore, these polysaccharides inhibited the early step of HCV infection but had no inhibitory effects on HTLV-1 replication. To further examine the specificity of these polysaccharides in viral infections, we used vesicular stomatitis virus (VSV)-G-pseudotyped HIV-1 infection. Ascophyllan, the two fucoidans, and alginate oligomer also potently inhibited VSV-G-pseudotyped HIV-1 infection in HeLa cells. Taken together, these results suggest that the acidic polysaccharides used in this study are capable of inhibiting the early step of viral infections depending on the polysaccharides but not in a strict species-specific manner.
KW - Acidic polysaccharide
KW - Hepatitis B virus (HBV)
KW - Human immunodeficiency virus type-1 (HIV-1)
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U2 - 10.1016/j.ijbiomac.2018.11.152
DO - 10.1016/j.ijbiomac.2018.11.152
M3 - Article
C2 - 30452989
AN - SCOPUS:85057333839
SN - 0141-8130
VL - 124
SP - 282
EP - 290
JO - International Journal of Biological Macromolecules
JF - International Journal of Biological Macromolecules
ER -
