Acidic calmodulin binding protein, ACAMP-81, is MARCKS protein interacting with synapsin I

Akihiro Mizutani; Hiroshi Tokumitsu; Hiroyoshi Hidaka

doi:10.1016/0006-291X(92)91888-W

Acidic calmodulin binding protein, ACAMP-81, is MARCKS protein interacting with synapsin I

Akihiro Mizutani, Hiroshi Tokumitsu, Hiroyoshi Hidaka

Research output: Contribution to journal › Article

4 Citations (Scopus)

Abstract

ACAMP-81 is an acidic calmodulin binding protein with molecular mass of 81 kDa. We report partial amino acid analysis of ACAMP-81 and its interaction with synapsin I. 123 amino acids of ACAMP-81 were determined and the sequence was completely identical with that of MARCKS protein which was thought to be a substrate for calcium/phopholipid dependent protein kinase (PKC). We found ACAMP-81 bound to synapsin I with ¹²⁵I-labeled ACAMP-81 overlay method. ACAMP-81 bound to the cysteine specific cleaved 51 kDa fragment derived from middle/tail region of synapsin I.

Original language	English
Pages (from-to)	1395-1401
Number of pages	7
Journal	Biochemical and Biophysical Research Communications
Volume	182
Issue number	3
DOIs	https://doi.org/10.1016/0006-291X(92)91888-W
Publication status	Published - Feb 14 1992
Externally published	Yes

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ASJC Scopus subject areas

Biophysics
Biochemistry
Molecular Biology
Cell Biology

Cite this

Mizutani, A., Tokumitsu, H., & Hidaka, H. (1992). Acidic calmodulin binding protein, ACAMP-81, is MARCKS protein interacting with synapsin I. Biochemical and Biophysical Research Communications, 182(3), 1395-1401. https://doi.org/10.1016/0006-291X(92)91888-W

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AB - ACAMP-81 is an acidic calmodulin binding protein with molecular mass of 81 kDa. We report partial amino acid analysis of ACAMP-81 and its interaction with synapsin I. 123 amino acids of ACAMP-81 were determined and the sequence was completely identical with that of MARCKS protein which was thought to be a substrate for calcium/phopholipid dependent protein kinase (PKC). We found ACAMP-81 bound to synapsin I with 125I-labeled ACAMP-81 overlay method. ACAMP-81 bound to the cysteine specific cleaved 51 kDa fragment derived from middle/tail region of synapsin I.

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10.1016/0006-291X(92)91888-W