Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma

Satsuki Aoyama, Ayako Masaki, Yuma Sakamoto, Hisashi Takino, Takayuki Murase, Koichi Ohshima, Tadashi Yoshino, Seiichi Kato, Hiroshi Inagaki

Research output: Contribution to journalArticle

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Abstract

Objective Achromobacter xylosoxidans (A. xylosoxidans) has been recently reported to have an association with the development of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma in patients from European countries. However, the prevalence rates for A. xylosoxidans may vary significantly from country to country. To assess this association, the prevalence of A. xylosoxidans was analyzed in Japanese patients with pulmonary B-cell lymphoma. Methods DNA samples were obtained from formalin-fixed, paraffin-embedded sections of pulmonary MALT lymphomas (n=52), diffuse large B-cell lymphomas (DLBCLs, n=18), and benign pulmonary lesions (n=19). All samples were histopathologically reviewed by experienced hematopathologists, and the clonality of all MALT lymphoma cases was confirmed by a polymerase chain reaction (PCR)-based IGH rearrangement clonality assay. They were also tested for the API2-MALT1 fusion transcript. The presence of bacterial DNA was assessed with a nested PCR, and DNA sequencing was performed to confirm the PCR specificity. Results A. xylosoxidans DNA was detected in 1/52 cases of pulmonary MALT lymphoma, 2/18 cases of DLBCL, and 0/19 cases of benign pulmonary lesions. The prevalence of A. xylosoxidans in pulmonary lymphoma was not significantly higher than in benign lesions. Conclusion The present study shows that A. xylosoxidans infection may not be associated with pulmonary B-cell lymphoma in a Japanese case series. Large-scale international studies are needed to clarify the role of A. xylosoxidans in pulmonary lymphoma.

Original languageEnglish
Pages (from-to)789-794
Number of pages6
JournalInternal Medicine
Volume57
Issue number6
DOIs
Publication statusPublished - Jan 1 2018

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Achromobacter
Achromobacter denitrificans
B-Cell Lymphoma
Lung
Marginal Zone B-Cell Lymphoma
Infection
Polymerase Chain Reaction
Lymphoma
Bacterial DNA
Lymphoma, Large B-Cell, Diffuse
DNA
DNA Sequence Analysis
Paraffin
Formaldehyde

Keywords

  • Achromobacter xylosoxidans
  • Geographical variability
  • Lung
  • Lymphoma

ASJC Scopus subject areas

  • Internal Medicine

Cite this

Aoyama, S., Masaki, A., Sakamoto, Y., Takino, H., Murase, T., Ohshima, K., ... Inagaki, H. (2018). Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma. Internal Medicine, 57(6), 789-794. https://doi.org/10.2169/internalmedicine.9430-17

Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma. / Aoyama, Satsuki; Masaki, Ayako; Sakamoto, Yuma; Takino, Hisashi; Murase, Takayuki; Ohshima, Koichi; Yoshino, Tadashi; Kato, Seiichi; Inagaki, Hiroshi.

In: Internal Medicine, Vol. 57, No. 6, 01.01.2018, p. 789-794.

Research output: Contribution to journalArticle

Aoyama, S, Masaki, A, Sakamoto, Y, Takino, H, Murase, T, Ohshima, K, Yoshino, T, Kato, S & Inagaki, H 2018, 'Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma', Internal Medicine, vol. 57, no. 6, pp. 789-794. https://doi.org/10.2169/internalmedicine.9430-17
Aoyama S, Masaki A, Sakamoto Y, Takino H, Murase T, Ohshima K et al. Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma. Internal Medicine. 2018 Jan 1;57(6):789-794. https://doi.org/10.2169/internalmedicine.9430-17
Aoyama, Satsuki ; Masaki, Ayako ; Sakamoto, Yuma ; Takino, Hisashi ; Murase, Takayuki ; Ohshima, Koichi ; Yoshino, Tadashi ; Kato, Seiichi ; Inagaki, Hiroshi. / Achromobacter infection is rare in Japanese patients with pulmonary B-cell lymphoma. In: Internal Medicine. 2018 ; Vol. 57, No. 6. pp. 789-794.
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abstract = "Objective Achromobacter xylosoxidans (A. xylosoxidans) has been recently reported to have an association with the development of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma in patients from European countries. However, the prevalence rates for A. xylosoxidans may vary significantly from country to country. To assess this association, the prevalence of A. xylosoxidans was analyzed in Japanese patients with pulmonary B-cell lymphoma. Methods DNA samples were obtained from formalin-fixed, paraffin-embedded sections of pulmonary MALT lymphomas (n=52), diffuse large B-cell lymphomas (DLBCLs, n=18), and benign pulmonary lesions (n=19). All samples were histopathologically reviewed by experienced hematopathologists, and the clonality of all MALT lymphoma cases was confirmed by a polymerase chain reaction (PCR)-based IGH rearrangement clonality assay. They were also tested for the API2-MALT1 fusion transcript. The presence of bacterial DNA was assessed with a nested PCR, and DNA sequencing was performed to confirm the PCR specificity. Results A. xylosoxidans DNA was detected in 1/52 cases of pulmonary MALT lymphoma, 2/18 cases of DLBCL, and 0/19 cases of benign pulmonary lesions. The prevalence of A. xylosoxidans in pulmonary lymphoma was not significantly higher than in benign lesions. Conclusion The present study shows that A. xylosoxidans infection may not be associated with pulmonary B-cell lymphoma in a Japanese case series. Large-scale international studies are needed to clarify the role of A. xylosoxidans in pulmonary lymphoma.",
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AU - Ohshima, Koichi

AU - Yoshino, Tadashi

AU - Kato, Seiichi

AU - Inagaki, Hiroshi

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N2 - Objective Achromobacter xylosoxidans (A. xylosoxidans) has been recently reported to have an association with the development of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma in patients from European countries. However, the prevalence rates for A. xylosoxidans may vary significantly from country to country. To assess this association, the prevalence of A. xylosoxidans was analyzed in Japanese patients with pulmonary B-cell lymphoma. Methods DNA samples were obtained from formalin-fixed, paraffin-embedded sections of pulmonary MALT lymphomas (n=52), diffuse large B-cell lymphomas (DLBCLs, n=18), and benign pulmonary lesions (n=19). All samples were histopathologically reviewed by experienced hematopathologists, and the clonality of all MALT lymphoma cases was confirmed by a polymerase chain reaction (PCR)-based IGH rearrangement clonality assay. They were also tested for the API2-MALT1 fusion transcript. The presence of bacterial DNA was assessed with a nested PCR, and DNA sequencing was performed to confirm the PCR specificity. Results A. xylosoxidans DNA was detected in 1/52 cases of pulmonary MALT lymphoma, 2/18 cases of DLBCL, and 0/19 cases of benign pulmonary lesions. The prevalence of A. xylosoxidans in pulmonary lymphoma was not significantly higher than in benign lesions. Conclusion The present study shows that A. xylosoxidans infection may not be associated with pulmonary B-cell lymphoma in a Japanese case series. Large-scale international studies are needed to clarify the role of A. xylosoxidans in pulmonary lymphoma.

AB - Objective Achromobacter xylosoxidans (A. xylosoxidans) has been recently reported to have an association with the development of pulmonary mucosa-associated lymphoid tissue (MALT) lymphoma in patients from European countries. However, the prevalence rates for A. xylosoxidans may vary significantly from country to country. To assess this association, the prevalence of A. xylosoxidans was analyzed in Japanese patients with pulmonary B-cell lymphoma. Methods DNA samples were obtained from formalin-fixed, paraffin-embedded sections of pulmonary MALT lymphomas (n=52), diffuse large B-cell lymphomas (DLBCLs, n=18), and benign pulmonary lesions (n=19). All samples were histopathologically reviewed by experienced hematopathologists, and the clonality of all MALT lymphoma cases was confirmed by a polymerase chain reaction (PCR)-based IGH rearrangement clonality assay. They were also tested for the API2-MALT1 fusion transcript. The presence of bacterial DNA was assessed with a nested PCR, and DNA sequencing was performed to confirm the PCR specificity. Results A. xylosoxidans DNA was detected in 1/52 cases of pulmonary MALT lymphoma, 2/18 cases of DLBCL, and 0/19 cases of benign pulmonary lesions. The prevalence of A. xylosoxidans in pulmonary lymphoma was not significantly higher than in benign lesions. Conclusion The present study shows that A. xylosoxidans infection may not be associated with pulmonary B-cell lymphoma in a Japanese case series. Large-scale international studies are needed to clarify the role of A. xylosoxidans in pulmonary lymphoma.

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