Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia

K. Tanaka, N. Ogawa, K. Mizukawa, Masato Asanuma, Y. Kondo, S. Nishibayashi, A. Mori

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Abstract

The effects of pre-treatment with ENA-713, an acetylcholinesterase (AChE) inhibitor, on changes in pre- and postsynaptic cholinergic indices in gerbil brain following transient ischemia were studied at 4 and 14 days after recirculation. In the ischemic group, hippocampal acetylcholine (ACh) level was significantly reduced (to 23% of sham-operated controls) at 4 days post-ischemia, but this reduction was completely prevented by ENA-713 treatment. Choline acetyltransferase (ChAT) and cholinesterase (ChE) activities were not significantly changed at 4 and 14 days post-ischemia. Although the maximum number (Bmax) of muscarinic ACh receptor (mACh-R) binding in the hippocampus was decreased (to 44%) without any change in affinity at 14 days post-ischemia, this decrease was also inhibited by ENA-713 treatment. In addition, histological experiment indicated that ENA-713 inhibited ischemia-induced pyramidal cell loss in the hippocampal CA1 regions. Thus, these findings suggest that ENA-713 has protective, neurotrophic and therapeutic effects on cerebrovascular type dementia due to cerebral ischemia.

Original languageEnglish
Pages (from-to)117-122
Number of pages6
JournalNeurochemical Research
Volume19
Issue number2
DOIs
Publication statusPublished - Feb 1994

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Rivastigmine
Gerbillinae
Cholinesterase Inhibitors
Cholinergic Agents
Brain
Ischemia
Hippocampal CA1 Region
Choline O-Acetyltransferase
Pyramidal Cells
Cholinesterases
Muscarinic Receptors
Therapeutic Uses
Brain Ischemia
Acetylcholine
Dementia
Hippocampus

Keywords

  • Acetylcholinesterase inhibitor
  • cholinergic indices
  • ENA-713
  • transient ischemia

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry

Cite this

Acetylcholinesterase inhibitor ENA-713 protects against ischemia-induced decrease in pre- and postsynaptic cholinergic indices in the gerbil brain following transient ischemia. / Tanaka, K.; Ogawa, N.; Mizukawa, K.; Asanuma, Masato; Kondo, Y.; Nishibayashi, S.; Mori, A.

In: Neurochemical Research, Vol. 19, No. 2, 02.1994, p. 117-122.

Research output: Contribution to journalArticle

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