Acetylcholine suppresses ventricular arrhythmias and improves conduction and connexin-43 properties during myocardial ischemia in isolated rabbit hearts

Takeshi Aiba, Takashi Noda, Ichiro Hidaka, Masashi Inagaki, Rajesh G. Katare, Motonori Ando, Kenji Sunagawa, Takayuki Sato, Masaru Sugimachi

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

ACh Prevents Ischemic Loss of Gj and Arrhythmias Introduction Acetylcholine (ACh), a vagal efferent neurotransmitter, markedly improves survival in rats with myocardial ischemia (MI) by preventing ischemic loss of gap junction (Gj) and by inducing anti-apoptotic cascades. However, electrophysiological mechanisms of the antiarrhythmic effect of ACh after acute MI are still unclear. Methods Acute MI was induced by ligation of the left anterior descending (LAD) coronary artery in Langendorff-perfused rabbit hearts with (ACh(+):n = 11) or without (ACh(-):n = 12) 10 μmol/L ACh delivered continuously starting at 5 minutes before LAD ligation. Action potentials on the left ventricular (LV) anterior surface (≈2×2 cm) were recorded by optical mapping during pacing from the LV epicardium (BCL = 500 milliseconds). Conduction velocities (CVs) at 256 sites were calculated and the ventricular tachycardia/ventricular fibrillation (VT/VF) susceptibility was also assessed by programmed electrical stimulation before and 30 minutes after MI. The amount and distribution of Gj protein connexin-43 was analyzed by immunoblotting and immunohistochemistry. Results Averaged CV in the ischemic border zone (IBZ) was significantly slower in ACh(-) than in ACh(+) (21 ± 7 vs. 34 ± 6 cm/s; P <0.01). Short-coupled extra stimulus further decreased CV of IBZ in ACh(-) (13 ± 4 cm/s) but did not change that in ACh(+) (34 ± 5 cm/s), leading to a high incidence of conduction block in IBZ in ACh(-) but not in ACh(+) (83% vs. 0%). VT/VF after MI were induced in ACh(-) but suppressed in ACh(+) (10/12 vs. 3/11; P <0.01). Connexin-43 in the LV anterior wall was significantly reduced after MI in ACh(-) but not in ACh(+). Conclusion ACh may suppress VT/VF by preventing loss of Gj and improving CV in IBZ during acute MI.

Original languageEnglish
Pages (from-to)678-685
Number of pages8
JournalJournal of Cardiovascular Electrophysiology
Volume26
Issue number6
DOIs
Publication statusPublished - Jun 1 2015
Externally publishedYes

Fingerprint

Connexin 43
Acetylcholine
Myocardial Ischemia
Cardiac Arrhythmias
Rabbits
Gap Junctions
Ventricular Fibrillation
Ventricular Tachycardia
Ligation
Connexins
Pericardium

Keywords

  • acetylcholine
  • conduction
  • connexin-43
  • gap junction
  • ischemia
  • optical mapping
  • ventricular arrhythmias

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Cite this

Acetylcholine suppresses ventricular arrhythmias and improves conduction and connexin-43 properties during myocardial ischemia in isolated rabbit hearts. / Aiba, Takeshi; Noda, Takashi; Hidaka, Ichiro; Inagaki, Masashi; Katare, Rajesh G.; Ando, Motonori; Sunagawa, Kenji; Sato, Takayuki; Sugimachi, Masaru.

In: Journal of Cardiovascular Electrophysiology, Vol. 26, No. 6, 01.06.2015, p. 678-685.

Research output: Contribution to journalArticle

Aiba, Takeshi ; Noda, Takashi ; Hidaka, Ichiro ; Inagaki, Masashi ; Katare, Rajesh G. ; Ando, Motonori ; Sunagawa, Kenji ; Sato, Takayuki ; Sugimachi, Masaru. / Acetylcholine suppresses ventricular arrhythmias and improves conduction and connexin-43 properties during myocardial ischemia in isolated rabbit hearts. In: Journal of Cardiovascular Electrophysiology. 2015 ; Vol. 26, No. 6. pp. 678-685.
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abstract = "ACh Prevents Ischemic Loss of Gj and Arrhythmias Introduction Acetylcholine (ACh), a vagal efferent neurotransmitter, markedly improves survival in rats with myocardial ischemia (MI) by preventing ischemic loss of gap junction (Gj) and by inducing anti-apoptotic cascades. However, electrophysiological mechanisms of the antiarrhythmic effect of ACh after acute MI are still unclear. Methods Acute MI was induced by ligation of the left anterior descending (LAD) coronary artery in Langendorff-perfused rabbit hearts with (ACh(+):n = 11) or without (ACh(-):n = 12) 10 μmol/L ACh delivered continuously starting at 5 minutes before LAD ligation. Action potentials on the left ventricular (LV) anterior surface (≈2×2 cm) were recorded by optical mapping during pacing from the LV epicardium (BCL = 500 milliseconds). Conduction velocities (CVs) at 256 sites were calculated and the ventricular tachycardia/ventricular fibrillation (VT/VF) susceptibility was also assessed by programmed electrical stimulation before and 30 minutes after MI. The amount and distribution of Gj protein connexin-43 was analyzed by immunoblotting and immunohistochemistry. Results Averaged CV in the ischemic border zone (IBZ) was significantly slower in ACh(-) than in ACh(+) (21 ± 7 vs. 34 ± 6 cm/s; P <0.01). Short-coupled extra stimulus further decreased CV of IBZ in ACh(-) (13 ± 4 cm/s) but did not change that in ACh(+) (34 ± 5 cm/s), leading to a high incidence of conduction block in IBZ in ACh(-) but not in ACh(+) (83{\%} vs. 0{\%}). VT/VF after MI were induced in ACh(-) but suppressed in ACh(+) (10/12 vs. 3/11; P <0.01). Connexin-43 in the LV anterior wall was significantly reduced after MI in ACh(-) but not in ACh(+). Conclusion ACh may suppress VT/VF by preventing loss of Gj and improving CV in IBZ during acute MI.",
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author = "Takeshi Aiba and Takashi Noda and Ichiro Hidaka and Masashi Inagaki and Katare, {Rajesh G.} and Motonori Ando and Kenji Sunagawa and Takayuki Sato and Masaru Sugimachi",
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T1 - Acetylcholine suppresses ventricular arrhythmias and improves conduction and connexin-43 properties during myocardial ischemia in isolated rabbit hearts

AU - Aiba, Takeshi

AU - Noda, Takashi

AU - Hidaka, Ichiro

AU - Inagaki, Masashi

AU - Katare, Rajesh G.

AU - Ando, Motonori

AU - Sunagawa, Kenji

AU - Sato, Takayuki

AU - Sugimachi, Masaru

PY - 2015/6/1

Y1 - 2015/6/1

N2 - ACh Prevents Ischemic Loss of Gj and Arrhythmias Introduction Acetylcholine (ACh), a vagal efferent neurotransmitter, markedly improves survival in rats with myocardial ischemia (MI) by preventing ischemic loss of gap junction (Gj) and by inducing anti-apoptotic cascades. However, electrophysiological mechanisms of the antiarrhythmic effect of ACh after acute MI are still unclear. Methods Acute MI was induced by ligation of the left anterior descending (LAD) coronary artery in Langendorff-perfused rabbit hearts with (ACh(+):n = 11) or without (ACh(-):n = 12) 10 μmol/L ACh delivered continuously starting at 5 minutes before LAD ligation. Action potentials on the left ventricular (LV) anterior surface (≈2×2 cm) were recorded by optical mapping during pacing from the LV epicardium (BCL = 500 milliseconds). Conduction velocities (CVs) at 256 sites were calculated and the ventricular tachycardia/ventricular fibrillation (VT/VF) susceptibility was also assessed by programmed electrical stimulation before and 30 minutes after MI. The amount and distribution of Gj protein connexin-43 was analyzed by immunoblotting and immunohistochemistry. Results Averaged CV in the ischemic border zone (IBZ) was significantly slower in ACh(-) than in ACh(+) (21 ± 7 vs. 34 ± 6 cm/s; P <0.01). Short-coupled extra stimulus further decreased CV of IBZ in ACh(-) (13 ± 4 cm/s) but did not change that in ACh(+) (34 ± 5 cm/s), leading to a high incidence of conduction block in IBZ in ACh(-) but not in ACh(+) (83% vs. 0%). VT/VF after MI were induced in ACh(-) but suppressed in ACh(+) (10/12 vs. 3/11; P <0.01). Connexin-43 in the LV anterior wall was significantly reduced after MI in ACh(-) but not in ACh(+). Conclusion ACh may suppress VT/VF by preventing loss of Gj and improving CV in IBZ during acute MI.

AB - ACh Prevents Ischemic Loss of Gj and Arrhythmias Introduction Acetylcholine (ACh), a vagal efferent neurotransmitter, markedly improves survival in rats with myocardial ischemia (MI) by preventing ischemic loss of gap junction (Gj) and by inducing anti-apoptotic cascades. However, electrophysiological mechanisms of the antiarrhythmic effect of ACh after acute MI are still unclear. Methods Acute MI was induced by ligation of the left anterior descending (LAD) coronary artery in Langendorff-perfused rabbit hearts with (ACh(+):n = 11) or without (ACh(-):n = 12) 10 μmol/L ACh delivered continuously starting at 5 minutes before LAD ligation. Action potentials on the left ventricular (LV) anterior surface (≈2×2 cm) were recorded by optical mapping during pacing from the LV epicardium (BCL = 500 milliseconds). Conduction velocities (CVs) at 256 sites were calculated and the ventricular tachycardia/ventricular fibrillation (VT/VF) susceptibility was also assessed by programmed electrical stimulation before and 30 minutes after MI. The amount and distribution of Gj protein connexin-43 was analyzed by immunoblotting and immunohistochemistry. Results Averaged CV in the ischemic border zone (IBZ) was significantly slower in ACh(-) than in ACh(+) (21 ± 7 vs. 34 ± 6 cm/s; P <0.01). Short-coupled extra stimulus further decreased CV of IBZ in ACh(-) (13 ± 4 cm/s) but did not change that in ACh(+) (34 ± 5 cm/s), leading to a high incidence of conduction block in IBZ in ACh(-) but not in ACh(+) (83% vs. 0%). VT/VF after MI were induced in ACh(-) but suppressed in ACh(+) (10/12 vs. 3/11; P <0.01). Connexin-43 in the LV anterior wall was significantly reduced after MI in ACh(-) but not in ACh(+). Conclusion ACh may suppress VT/VF by preventing loss of Gj and improving CV in IBZ during acute MI.

KW - acetylcholine

KW - conduction

KW - connexin-43

KW - gap junction

KW - ischemia

KW - optical mapping

KW - ventricular arrhythmias

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