Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos

Ken ichi Yamanaka, Satoshi Sugimura, Takuya Wakai, Manabu Kawahara, Eimei Sato

Research output: Contribution to journalArticle

66 Citations (Scopus)

Abstract

Successful cloning by somatic cell nuclear transfer (SCNT) requires a reprogramming process in which the epigenetic state of a differentiated donor nucleus must be converted into an embryonic totipotent state. However, this epigenetic reprogramming is incomplete in SCNT embryos, causing low production efficiency. Recently, it has been reported that trichostatin A (TSA), an inhibitor of histone deacetylase, potentially enhances cloning efficiency. The aim of the present study was to optimize the TSA treatment for miniature pig SCNT embryos and investigate the effect of the acetylation level of histone on developmental competence of SCNT embryos. In order to optimize the TSA treatment, we examined the developmental competence of SCNT embryos under various exposure times (0-50 h) and concentrations (0-500 nM). Treatment with 5 nM TSA for 15 and 20 h beginning at the start of activation significantly increased the blastocyst formation rate (34.6 and 32.4 vs. 18.2%, respectively) and mean cell number (57.0 ± 2.7 and 56.6 ± 2.7 vs. 43.5 ± 2.1, respectively) as compared with the non-treated group (0 h). We then investigated the acetylation levels of histone H3 in SCNT embryos treated with or without TSA (TSA (+) or TSA (-)) as compared with in vitro-fertilized (IVF) embryos. The acetylation levels of the TSA (-) SCNT embryos at the pseudo-pronuclear and 2-cell stages were significantly lower than those of the IVF embryos at the same developmental stages. In contrast, the acetylation levels of the TSA (+) SCNT embryos were similar to those of the IVF embryos. There was no difference in the acetylation levels of all groups at the blastocyst stage. Our data therefore suggests that the acetylation level of histone H3 at the pseudo-pronuclear and 2-cell stages is positively correlated with subsequent development of SCNT embryos, which may be an important event for the vital development of SCNT embryos in miniature pigs.

Original languageEnglish
Pages (from-to)638-644
Number of pages7
JournalJournal of Reproduction and Development
Volume55
Issue number6
DOIs
Publication statusPublished - Dec 2009
Externally publishedYes

Fingerprint

miniature swine
acetylation
embryo transfer
somatic cells
histones
embryo (animal)
epigenetics
blastocyst
molecular cloning
histone deacetylase
cells
exposure duration
developmental stages

Keywords

  • Embryo development
  • Histone acetylation
  • Miniature pig
  • Nuclear transfer
  • Trichostatin A

ASJC Scopus subject areas

  • Animal Science and Zoology

Cite this

Acetylation level of histone H3 in early embryonic stages affects subsequent development of miniature pig somatic cell nuclear transfer embryos. / Yamanaka, Ken ichi; Sugimura, Satoshi; Wakai, Takuya; Kawahara, Manabu; Sato, Eimei.

In: Journal of Reproduction and Development, Vol. 55, No. 6, 12.2009, p. 638-644.

Research output: Contribution to journalArticle

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