Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening

Hideki Hanada, Hirotsugu Kobuchi, Masanao Yamamoto, Keiko Kashiwagi, Kenjiro Katsu, Toshihiko Utsumi, Akihiko Kashiwagi, Junzo Sasaki, Masayasu Inoue, Kozo Utsumi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Mitochondrial membrane permeability transition (MPT) plays a crucial role in apoptotic tail shortening during anuran metamor phosis. L-carnitine is known to shuttle free fatty acids (FFAs) from the cytosol into mitochondria matrix for β-oxidation and energy production, and in a previous study we found that treatment with L-carnitine suppresses 3, 3', 5-triiodothyronine (T3) and FFA-induced MPT by reducing the level of FFAs. In the present study we focus on acetyl-L-carnitine, which is also involved in fatty acid oxidation, to determine its effect on T3-induced tail regression in Rana rugosa tadpoles and spontaneous tail regression in Xenopus laevis tadpoles. The ladder-like DNA profile and increases in caspase-3 and caspase-9 indicative of apoptosis in the tails of T3-treated tadpoles were found to be suppressed by the addition of acetyl-L-carnitine. Likewise, acetyl-L-carnitine was found to inhibit thyroid hormone regulated spontaneous metamorphosis in X. laevis tadpoles, accompanied by decreases in caspase and phospholipase A2 activity, as well as non-ladder-like DNA profiles. These findings support our previous conclusion that elevated levels of FFAs initiate MPT and activate the signaling pathway controlling apoptotic cell death in tadpole tails during anuran metamorphosis.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalHereditas
Volume150
Issue number1
DOIs
Publication statusPublished - Feb 2013

Fingerprint

Acetylcarnitine
Thyroid Hormones
Larva
Tail
Nonesterified Fatty Acids
Permeability
Carnitine
Xenopus laevis
Reverse Triiodothyronine
Ranidae
Membranes
Caspase 9
Phospholipases A2
DNA
Mitochondrial Membranes
Caspases
Caspase 3
Cytosol
Mitochondria
Cell Death

ASJC Scopus subject areas

  • Genetics

Cite this

Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening. / Hanada, Hideki; Kobuchi, Hirotsugu; Yamamoto, Masanao; Kashiwagi, Keiko; Katsu, Kenjiro; Utsumi, Toshihiko; Kashiwagi, Akihiko; Sasaki, Junzo; Inoue, Masayasu; Utsumi, Kozo.

In: Hereditas, Vol. 150, No. 1, 02.2013, p. 1-9.

Research output: Contribution to journalArticle

Hanada, H, Kobuchi, H, Yamamoto, M, Kashiwagi, K, Katsu, K, Utsumi, T, Kashiwagi, A, Sasaki, J, Inoue, M & Utsumi, K 2013, 'Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening', Hereditas, vol. 150, no. 1, pp. 1-9. https://doi.org/10.1111/j.1601-5223.2013.02284.x
Hanada, Hideki ; Kobuchi, Hirotsugu ; Yamamoto, Masanao ; Kashiwagi, Keiko ; Katsu, Kenjiro ; Utsumi, Toshihiko ; Kashiwagi, Akihiko ; Sasaki, Junzo ; Inoue, Masayasu ; Utsumi, Kozo. / Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening. In: Hereditas. 2013 ; Vol. 150, No. 1. pp. 1-9.
@article{65b67af07ae74cfdadf860c774f6facf,
title = "Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening",
abstract = "Mitochondrial membrane permeability transition (MPT) plays a crucial role in apoptotic tail shortening during anuran metamor phosis. L-carnitine is known to shuttle free fatty acids (FFAs) from the cytosol into mitochondria matrix for β-oxidation and energy production, and in a previous study we found that treatment with L-carnitine suppresses 3, 3', 5-triiodothyronine (T3) and FFA-induced MPT by reducing the level of FFAs. In the present study we focus on acetyl-L-carnitine, which is also involved in fatty acid oxidation, to determine its effect on T3-induced tail regression in Rana rugosa tadpoles and spontaneous tail regression in Xenopus laevis tadpoles. The ladder-like DNA profile and increases in caspase-3 and caspase-9 indicative of apoptosis in the tails of T3-treated tadpoles were found to be suppressed by the addition of acetyl-L-carnitine. Likewise, acetyl-L-carnitine was found to inhibit thyroid hormone regulated spontaneous metamorphosis in X. laevis tadpoles, accompanied by decreases in caspase and phospholipase A2 activity, as well as non-ladder-like DNA profiles. These findings support our previous conclusion that elevated levels of FFAs initiate MPT and activate the signaling pathway controlling apoptotic cell death in tadpole tails during anuran metamorphosis.",
author = "Hideki Hanada and Hirotsugu Kobuchi and Masanao Yamamoto and Keiko Kashiwagi and Kenjiro Katsu and Toshihiko Utsumi and Akihiko Kashiwagi and Junzo Sasaki and Masayasu Inoue and Kozo Utsumi",
year = "2013",
month = "2",
doi = "10.1111/j.1601-5223.2013.02284.x",
language = "English",
volume = "150",
pages = "1--9",
journal = "Hereditas",
issn = "0018-0661",
publisher = "Wiley-Blackwell",
number = "1",

}

TY - JOUR

T1 - Acetyl-L-carnitine suppresses thyroid hormone-induced and spontaneous anuran tadpole tail shortening

AU - Hanada, Hideki

AU - Kobuchi, Hirotsugu

AU - Yamamoto, Masanao

AU - Kashiwagi, Keiko

AU - Katsu, Kenjiro

AU - Utsumi, Toshihiko

AU - Kashiwagi, Akihiko

AU - Sasaki, Junzo

AU - Inoue, Masayasu

AU - Utsumi, Kozo

PY - 2013/2

Y1 - 2013/2

N2 - Mitochondrial membrane permeability transition (MPT) plays a crucial role in apoptotic tail shortening during anuran metamor phosis. L-carnitine is known to shuttle free fatty acids (FFAs) from the cytosol into mitochondria matrix for β-oxidation and energy production, and in a previous study we found that treatment with L-carnitine suppresses 3, 3', 5-triiodothyronine (T3) and FFA-induced MPT by reducing the level of FFAs. In the present study we focus on acetyl-L-carnitine, which is also involved in fatty acid oxidation, to determine its effect on T3-induced tail regression in Rana rugosa tadpoles and spontaneous tail regression in Xenopus laevis tadpoles. The ladder-like DNA profile and increases in caspase-3 and caspase-9 indicative of apoptosis in the tails of T3-treated tadpoles were found to be suppressed by the addition of acetyl-L-carnitine. Likewise, acetyl-L-carnitine was found to inhibit thyroid hormone regulated spontaneous metamorphosis in X. laevis tadpoles, accompanied by decreases in caspase and phospholipase A2 activity, as well as non-ladder-like DNA profiles. These findings support our previous conclusion that elevated levels of FFAs initiate MPT and activate the signaling pathway controlling apoptotic cell death in tadpole tails during anuran metamorphosis.

AB - Mitochondrial membrane permeability transition (MPT) plays a crucial role in apoptotic tail shortening during anuran metamor phosis. L-carnitine is known to shuttle free fatty acids (FFAs) from the cytosol into mitochondria matrix for β-oxidation and energy production, and in a previous study we found that treatment with L-carnitine suppresses 3, 3', 5-triiodothyronine (T3) and FFA-induced MPT by reducing the level of FFAs. In the present study we focus on acetyl-L-carnitine, which is also involved in fatty acid oxidation, to determine its effect on T3-induced tail regression in Rana rugosa tadpoles and spontaneous tail regression in Xenopus laevis tadpoles. The ladder-like DNA profile and increases in caspase-3 and caspase-9 indicative of apoptosis in the tails of T3-treated tadpoles were found to be suppressed by the addition of acetyl-L-carnitine. Likewise, acetyl-L-carnitine was found to inhibit thyroid hormone regulated spontaneous metamorphosis in X. laevis tadpoles, accompanied by decreases in caspase and phospholipase A2 activity, as well as non-ladder-like DNA profiles. These findings support our previous conclusion that elevated levels of FFAs initiate MPT and activate the signaling pathway controlling apoptotic cell death in tadpole tails during anuran metamorphosis.

UR - http://www.scopus.com/inward/record.url?scp=84875081988&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875081988&partnerID=8YFLogxK

U2 - 10.1111/j.1601-5223.2013.02284.x

DO - 10.1111/j.1601-5223.2013.02284.x

M3 - Article

C2 - 23489246

AN - SCOPUS:84875081988

VL - 150

SP - 1

EP - 9

JO - Hereditas

JF - Hereditas

SN - 0018-0661

IS - 1

ER -