Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats

Akari Ishisaka, Satomi Ichikawa, Hiroyuki Sakakibara, Mariusz K. Piskula, Toshiyuki Nakamura, Yoji Kato, Mikiko Ito, Ken Ichi Miyamoto, Akira Tsuji, Yoshichika Kawai, Junji Terao

Research output: Contribution to journalArticle

115 Citations (Scopus)

Abstract

Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with β-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress.

Original languageEnglish
Pages (from-to)1329-1336
Number of pages8
JournalFree Radical Biology and Medicine
Volume51
Issue number7
DOIs
Publication statusPublished - Oct 1 2011
Externally publishedYes

Fingerprint

Quercetin
Rats
Brain
Tissue
High performance liquid chromatography
High Pressure Liquid Chromatography
Oxidative stress
Oxidative Stress
Sulfatases
Glucuronidase
Endothelial cells
Vegetables
Neuroprotective Agents
Tandem Mass Spectrometry
Bioactivity
Blood-Brain Barrier
Nutrients
Mass spectrometry
Oral Administration
Hippocampus

Keywords

  • Blood-brain barrier
  • Brain
  • Chromatography
  • Free radicals
  • Oxidative stress
  • Quercetin

ASJC Scopus subject areas

  • Biochemistry
  • Physiology (medical)

Cite this

Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats. / Ishisaka, Akari; Ichikawa, Satomi; Sakakibara, Hiroyuki; Piskula, Mariusz K.; Nakamura, Toshiyuki; Kato, Yoji; Ito, Mikiko; Miyamoto, Ken Ichi; Tsuji, Akira; Kawai, Yoshichika; Terao, Junji.

In: Free Radical Biology and Medicine, Vol. 51, No. 7, 01.10.2011, p. 1329-1336.

Research output: Contribution to journalArticle

Ishisaka, A, Ichikawa, S, Sakakibara, H, Piskula, MK, Nakamura, T, Kato, Y, Ito, M, Miyamoto, KI, Tsuji, A, Kawai, Y & Terao, J 2011, 'Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats', Free Radical Biology and Medicine, vol. 51, no. 7, pp. 1329-1336. https://doi.org/10.1016/j.freeradbiomed.2011.06.017
Ishisaka, Akari ; Ichikawa, Satomi ; Sakakibara, Hiroyuki ; Piskula, Mariusz K. ; Nakamura, Toshiyuki ; Kato, Yoji ; Ito, Mikiko ; Miyamoto, Ken Ichi ; Tsuji, Akira ; Kawai, Yoshichika ; Terao, Junji. / Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats. In: Free Radical Biology and Medicine. 2011 ; Vol. 51, No. 7. pp. 1329-1336.
@article{447e465525e54fe9909defdbc01494dd,
title = "Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats",
abstract = "Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with β-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress.",
keywords = "Blood-brain barrier, Brain, Chromatography, Free radicals, Oxidative stress, Quercetin",
author = "Akari Ishisaka and Satomi Ichikawa and Hiroyuki Sakakibara and Piskula, {Mariusz K.} and Toshiyuki Nakamura and Yoji Kato and Mikiko Ito and Miyamoto, {Ken Ichi} and Akira Tsuji and Yoshichika Kawai and Junji Terao",
year = "2011",
month = "10",
day = "1",
doi = "10.1016/j.freeradbiomed.2011.06.017",
language = "English",
volume = "51",
pages = "1329--1336",
journal = "Free Radical Biology and Medicine",
issn = "0891-5849",
publisher = "Elsevier Inc.",
number = "7",

}

TY - JOUR

T1 - Accumulation of orally administered quercetin in brain tissue and its antioxidative effects in rats

AU - Ishisaka, Akari

AU - Ichikawa, Satomi

AU - Sakakibara, Hiroyuki

AU - Piskula, Mariusz K.

AU - Nakamura, Toshiyuki

AU - Kato, Yoji

AU - Ito, Mikiko

AU - Miyamoto, Ken Ichi

AU - Tsuji, Akira

AU - Kawai, Yoshichika

AU - Terao, Junji

PY - 2011/10/1

Y1 - 2011/10/1

N2 - Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with β-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress.

AB - Quercetin is widely distributed in vegetables and herbs and has been suggested to act as a neuroprotective agent. Here, we demonstrate that quercetin can accumulate enough to exert biological activity in rat brain tissues. Homogenates of perfused rat brain without detectable hemoglobin contaminants were treated with β-glucuronidase/sulfatase and the released quercetin and its methylated form were analyzed using high-performance liquid chromatography (HPLC) with three different detection methods. Both quercetin and the methylated form were detected in the brain of quercetin-administered rats using HPLC-UV and HPLC with electrochemical detection and were further identified using HPLC-tandem mass spectrometry. Oral administration of quercetin (50 mg/kg body wt) attenuated the increased oxidative stress in the hippocampus and striatum of rats exposed to chronic forced swimming. The possible transport of quercetin derivatives into the brain tissue was reproduced in vitro by using a rat brain capillary endothelial cell line, a model of the blood-brain barrier. These results show that quercetin could be a potent nutrient that can access the brain and protect it from disorders associated with oxidative stress.

KW - Blood-brain barrier

KW - Brain

KW - Chromatography

KW - Free radicals

KW - Oxidative stress

KW - Quercetin

UR - http://www.scopus.com/inward/record.url?scp=80052269348&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=80052269348&partnerID=8YFLogxK

U2 - 10.1016/j.freeradbiomed.2011.06.017

DO - 10.1016/j.freeradbiomed.2011.06.017

M3 - Article

C2 - 21741473

AN - SCOPUS:80052269348

VL - 51

SP - 1329

EP - 1336

JO - Free Radical Biology and Medicine

JF - Free Radical Biology and Medicine

SN - 0891-5849

IS - 7

ER -