Accumulation of amyloid β protein in Tg2576 mice

Takeshi Kawarabayashi, Tetsuro Murakami, Etsuro Matsubara, Mikio Shoji, Koji Abe

Research output: Contribution to journalArticle

Abstract

The progressive deposition of amyloid β protein (Aβ) is a crucial step of Alzheimer's disease (AD). To detect when and where Aβ begins to deposit, we examined fractions by sucrose gradient centrifugation from Tg2576 mouse brains, an animal model of AD. Precursors of Aβ, β-secretase and putative γ-secretase were all recovered in lipid rafts, which are low density, detergent-insoluble membrane fractions. The major Aβ catabolizing enzyme, neprilysin, and apolipoprotein E also co-localized in lipid rafts. In 3-month-old Tg2576, about 20% of total Aβ40 and Aβ42 were detected in lipid rafts. From 7–8 months, the early stage of amyloid deposition, Aβ42 increased in lipid rafts. These results suggested that lipid rafts may be crucial sites for Aβ production, Aβ degradation and Aβ aggregation leading to early accumulation of Aβ amyloid.

Original languageEnglish
Pages (from-to)373-377
Number of pages5
JournalInternational Congress Series
Volume1252
Issue numberC
DOIs
Publication statusPublished - Jun 1 2003

Fingerprint

Amyloidogenic Proteins
Lipids
Amyloid Precursor Protein Secretases
Amyloid
Alzheimer Disease
Serum Amyloid A Protein
Neprilysin
Apolipoproteins E
Centrifugation
Detergents
Sucrose
Animal Models
Membranes
Brain
Enzymes

Keywords

  • Alzheimer's disease
  • Amyloid
  • Dementia
  • Transgenic mouse
  • β protein

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Accumulation of amyloid β protein in Tg2576 mice. / Kawarabayashi, Takeshi; Murakami, Tetsuro; Matsubara, Etsuro; Shoji, Mikio; Abe, Koji.

In: International Congress Series, Vol. 1252, No. C, 01.06.2003, p. 373-377.

Research output: Contribution to journalArticle

Kawarabayashi, T, Murakami, T, Matsubara, E, Shoji, M & Abe, K 2003, 'Accumulation of amyloid β protein in Tg2576 mice', International Congress Series, vol. 1252, no. C, pp. 373-377. https://doi.org/10.1016/S0531-5131(03)00015-3
Kawarabayashi, Takeshi ; Murakami, Tetsuro ; Matsubara, Etsuro ; Shoji, Mikio ; Abe, Koji. / Accumulation of amyloid β protein in Tg2576 mice. In: International Congress Series. 2003 ; Vol. 1252, No. C. pp. 373-377.
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