Abstract
The progressive deposition of amyloid β protein (Aβ) is a crucial step of Alzheimer's disease (AD). To detect when and where Aβ begins to deposit, we examined fractions by sucrose gradient centrifugation from Tg2576 mouse brains, an animal model of AD. Precursors of Aβ, β-secretase and putative γ-secretase were all recovered in lipid rafts, which are low density, detergent-insoluble membrane fractions. The major Aβ catabolizing enzyme, neprilysin, and apolipoprotein E also co-localized in lipid rafts. In 3-month-old Tg2576, about 20% of total Aβ40 and Aβ42 were detected in lipid rafts. From 7–8 months, the early stage of amyloid deposition, Aβ42 increased in lipid rafts. These results suggested that lipid rafts may be crucial sites for Aβ production, Aβ degradation and Aβ aggregation leading to early accumulation of Aβ amyloid.
| Original language | English |
|---|---|
| Pages (from-to) | 373-377 |
| Number of pages | 5 |
| Journal | International Congress Series |
| Volume | 1252 |
| Issue number | C |
| DOIs | |
| Publication status | Published - Jun 1 2003 |
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Keywords
- Alzheimer's disease
- Amyloid
- Dementia
- Transgenic mouse
- β protein
ASJC Scopus subject areas
- Medicine(all)
Cite this
Accumulation of amyloid β protein in Tg2576 mice. / Kawarabayashi, Takeshi; Murakami, Tetsuro; Matsubara, Etsuro; Shoji, Mikio; Abe, Koji.
In: International Congress Series, Vol. 1252, No. C, 01.06.2003, p. 373-377.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Accumulation of amyloid β protein in Tg2576 mice
AU - Kawarabayashi, Takeshi
AU - Murakami, Tetsuro
AU - Matsubara, Etsuro
AU - Shoji, Mikio
AU - Abe, Koji
PY - 2003/6/1
Y1 - 2003/6/1
N2 - The progressive deposition of amyloid β protein (Aβ) is a crucial step of Alzheimer's disease (AD). To detect when and where Aβ begins to deposit, we examined fractions by sucrose gradient centrifugation from Tg2576 mouse brains, an animal model of AD. Precursors of Aβ, β-secretase and putative γ-secretase were all recovered in lipid rafts, which are low density, detergent-insoluble membrane fractions. The major Aβ catabolizing enzyme, neprilysin, and apolipoprotein E also co-localized in lipid rafts. In 3-month-old Tg2576, about 20% of total Aβ40 and Aβ42 were detected in lipid rafts. From 7–8 months, the early stage of amyloid deposition, Aβ42 increased in lipid rafts. These results suggested that lipid rafts may be crucial sites for Aβ production, Aβ degradation and Aβ aggregation leading to early accumulation of Aβ amyloid.
AB - The progressive deposition of amyloid β protein (Aβ) is a crucial step of Alzheimer's disease (AD). To detect when and where Aβ begins to deposit, we examined fractions by sucrose gradient centrifugation from Tg2576 mouse brains, an animal model of AD. Precursors of Aβ, β-secretase and putative γ-secretase were all recovered in lipid rafts, which are low density, detergent-insoluble membrane fractions. The major Aβ catabolizing enzyme, neprilysin, and apolipoprotein E also co-localized in lipid rafts. In 3-month-old Tg2576, about 20% of total Aβ40 and Aβ42 were detected in lipid rafts. From 7–8 months, the early stage of amyloid deposition, Aβ42 increased in lipid rafts. These results suggested that lipid rafts may be crucial sites for Aβ production, Aβ degradation and Aβ aggregation leading to early accumulation of Aβ amyloid.
KW - Alzheimer's disease
KW - Amyloid
KW - Dementia
KW - Transgenic mouse
KW - β protein
UR - http://www.scopus.com/inward/record.url?scp=85023077424&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85023077424&partnerID=8YFLogxK
U2 - 10.1016/S0531-5131(03)00015-3
DO - 10.1016/S0531-5131(03)00015-3
M3 - Article
AN - SCOPUS:85023077424
VL - 1252
SP - 373
EP - 377
JO - International Congress Series
JF - International Congress Series
SN - 0531-5131
IS - C
ER -